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International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Thu, Apr 25, 2024
[Archive]
Volume 1, Issue 1 (December 2014)
IJML 2014, 1(1): 7-14 |
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Asadi M, Hadinedoushan H, Mirghanizadeh S A, Karimollah A, Dashti F, Malek-hosseini S. The Effect of Echinops Lasiolepis Extracts, Native Plant of Yazd Province, on Peripheral Blood Mononuclear Cell Proliferation and IFN-γ Secretion. IJML 2014; 1 (1) :7-14
URL: http://ijml.ssu.ac.ir/article-1-24-en.html
URL: http://ijml.ssu.ac.ir/article-1-24-en.html
Maryam Asadi 
, Hossein Hadinedoushan * , Seyed Ali Mirghanizadeh , Alireza Karimollah , Fatemehsadat Dashti , Saeid Malek-hosseini
, Hossein Hadinedoushan * , Seyed Ali Mirghanizadeh , Alireza Karimollah , Fatemehsadat Dashti , Saeid Malek-hosseini
Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract: (5018 Views)
Background and Aims: The present study was performed to investigate the effect of Echinops lasiolepis extract on peripheral blood mononuclear cells (PBMCs) proliferation and interferon (IFN)-&gamma secretion.
Materials and Methods: PBMCs were obtained from three healthy volunteers and cultured in presence of Echinops lasiolepis methanol extract in different concentrations of 0.1, 1, 10, 100 and 200 µg/ml. Lipopolysacharide (LPS) and Phytohaemagglutinin (PHA) were considered as mitogen. BrdU cell proliferation assay was used to determine cell proliferation. The concentration of IFN-&gamma in the PBMCs supernatant was determined using enzyme-linked immunosorbent assay.
Results: Most concentrations of Echinops lasiolepis methanol extract showed inhibitory effect on PBMCs proliferation. There were significant differences between the concentrations of 200, 1 and 0.1 µg/ml (p<0.05). The optimum proliferation (30.66±24.67%) was obtained at concentration 1 µg/ml. The PBMCs supernatant IFN-&gamma concentrations in extract treated group without PHA and LPS and in PHA activated group were not significantly different.
Conclusions: Our findings indicated that Echinops lasiolepis extract has immunomodulating activity which may be potentialy to used as an immunotherapy agent.
Type of Study: Research |
Subject:
General
Received: 2014/12/23 | Accepted: 2014/12/23 | Published: 2014/12/23
Received: 2014/12/23 | Accepted: 2014/12/23 | Published: 2014/12/23
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