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International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Fri, Apr 26, 2024
[Archive]
Volume 2, Issue 2 (August 2015)
IJML 2015, 2(2): 105-111 |
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Eskandarzade N, Heydarnezhad A, Valizadeh Y, Shokouhi M, Shafizadeh Kholenjani A. Molecular Detection of Chicken Anemia Virus from Native Larry-breed Chickens in Chaharmahal-va-Bakhtiyari Province, Iran. IJML 2015; 2 (2) :105-111
URL: http://ijml.ssu.ac.ir/article-1-54-en.html
URL: http://ijml.ssu.ac.ir/article-1-54-en.html
Neda Eskandarzade * 
, Alireza Heydarnezhad , Yousef Valizadeh , Mona Shokouhi , Alireza Shafizadeh Kholenjani
, Alireza Heydarnezhad , Yousef Valizadeh , Mona Shokouhi , Alireza Shafizadeh Kholenjani
Department of Biochemistry, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Abstract: (3020 Views)
Background and Aims: Finding prevalence of chicken anemia virus (CAV) infection in native chickens is necessary to avoid transmission of infection to commercial flocks. In this paper we attempt to describe molecular detection of chicken anemia virus in native Larry-breed chickens in Chaharmahal-va-Bakhtiyari province in Iran for the first time.
Materials and Methods: Blood samples were collected from 100 native Larry-breed chickens (5-8 months old) in Shahrekord, Lordegan, Brogen and Koohrang, i.e.four cities of Chaharmahal-va-Bakhtiyari province. To detect CAV, Polymerase chain reaction (PCR) was undertaken on isolated DNA from blood samples using a pair of CAV specific primers that produced a 374 base pair fragment.
Results: PCR analysis detected CAV in 12 of 100 (12%) tested blood samples.
Conclusions: The results revealed that native Larry chicks were not free from CAV infection in Chaharmahal-va-Bakhtiyari province and vaccination against CAV should be taken into account in native farms.
Type of Study: Research |
Subject:
General
Received: 2015/08/18 | Accepted: 2015/08/18 | Published: 2015/08/18
Received: 2015/08/18 | Accepted: 2015/08/18 | Published: 2015/08/18
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