International Journal of Medical Laboratory

en Pulmonoprotective Action of Zataria multiflora in an experimental model of lung inflammation induced by Ochratoxin-A in mice Pulmonoprotective Action of Zataria Multiflora in an Experimental Model of Lung Inflammation Induced by Ochratoxin-A in Mice قارچ شناسی Mycology پژوهشي Research <div dir="ltr" style="text-align: justify;">Ochratoxin A (OTA) is one of the most important secondary metabolites of Penicillium and Aspergillus. Acratoxin was recognized in 1965 as the most important carcinogenic mycotoxin in agricultural products. Due to the key role of the lungs in maintaining the function of other organs of the body, we examined the role of the Zataria multiflora extract (ZM) in an experimental model of lung inflammation induced by Ochratoxin-A in mice. Hence the study was designed with 14-day gavages of OTA in mice. Markers of oxidative stress and apoptotic factors (Bax/Bcl2, Caspase 3 Caspase 9) were also evaluated. The results revealed that OTA toxicity induces lung inflammation and apoptosis in a dose-dependent manner. Administration of Administration of Zataria multiflora (25 and 50 mg/kg) significantly alleviated lung inflammation, oxidative damage, inflammatory cytokines and apoptosis protein levels compared to OTA. In conclusion, the results of the study show that Zataria multiflora has strong antioxidant effects against inflammation and apoptosis of OTA toxicity in lung tissue.</div> <div style="text-align: justify;"></div> <div style="text-align: justify;">Introduction: Ochratoxin-A (OTA) represents one of the primary secondary metabolites produced by Penicillium and Aspergillus species. Acratoxin was recognized in 1965 as the most significant carcinogenic mycotoxin found in agricultural products. Given that the vital function of the lungs in maintaining other bodily organ system, it was examined the role of the Zataria multiflora extract in an experimental model of lung inflammation induced by Ochratoxin-A in mice. Materials and Methods: This study was designed around a 14-day gavage administration of OTA in mice. Markers of oxidative stress and apoptotic factors (Bax/Bcl2, Caspase 3 Caspase 9) were also evaluated. Results: OTA-induced toxicity provokes lung inflammation and apoptosis in a dose-dependent manner. The administration of Zataria multiflora (at dosages of 25 and 50 mg/kg) resulted in a significant alleviated of lung inflammation, oxidative damage, inflammatory cytokines, and levels of apoptosis protein when compared to the group treated with OTA alone. Conclusion: The results of the study indicate that Zataria multiflora exerts robust antioxidant effects that counteract the inflammation and apoptosis associated with OTA-induced toxicity in lung tissue.</div> Ochratoxin A, Zataria multiflora, Apoptosis pathway, apoptosis, Lung inflammation. Apoptosis, Apoptosis pathway, Lung inflammation, Ochratoxin A, Zataria multiflora 0 0 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-545-1&slc_lang=en&sid=1 Fahimeh Nourbakhsh فهیمه نوربخش fahimeh_nourbakhsh@yahoo.com No Phd of toxicology, deputy of food and drug, Isfahan University of Medical Sciences, Isfahan, Iran. کارشناس معاونت غذا و دارو، دانشگاه علوم پزشکی اصفهان Hossein Chahardah Masoumi حسین چهارده معصومی fn.nourbakhsh@gmail.com No Doctor of veterinary medicine, deputy of food and drug, Isfahan University of Medical Sciences, Isfahan, Iran. دکتری دامپزشکی، کارشناس معاونت غذا و دارو، دانشگاه علوم پزشکی اصفهان. Mohammadreza Saebi محمد رضا صائبی No Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Elaheh Tajbakhsh الهه تاج بخش ee_tajbakhsh@gmail.com No 4. Department of microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. استاد میکروبیولوژی، دانشگاه ازاد اسلامی واحد شهرکرد،شهرکرد ایران