Volume 5, Issue 1 (February 2018)                   IJML 2018, 5(1): 35-41 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Harir Foroush M, Shokoohizadeh L, Mirzaee M. Prevalence of Genes Encoding Aminoglycoside Modifying Enzymes in Clinical Isolates of Klebsiella Pneumoniae in the Hospitals of Borujerd . IJML. 2018; 5 (1) :35-41
URL: http://ijml.ssu.ac.ir/article-1-142-en.html
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract:   (1198 Views)

Background and Aims: Given the importance of aminoglycoside resistance in nosocomial and community infections caused by bacterial pathogenes such as Klebsiella pneumoniae (K. pneumoniae), the aim of this study was to determine the frequency of aac (6')- Ib and aac (3)- IIa, the genes encoding aminoglycoside modifying enzymes involved in aminoglycoside resistance.
Material and Methods: A total of 100 K. pneumonia isolates were collected from hospitalized patients from April to September 2015 in Borujerd hospitals. Conventional microbiological tests were carried out to detect and confirm K. pneumonia isolates. Antibiotic susceptibility of isolates was detected by disk diffusion methods. The presence of the aac(6')-Ib and aac(3)-IIa genes which encode aminoglycoside modifying enzymes was determined by polymerase chain reaction.
Results: Among 100 K. pneumonia isolates, 34% showed resistance to gentamicin and 21% to amikacin. Resistance to both gentamicin and amikacin was detected in 18% of the isolates. Multi-resistance phenotypes were detected in 71% of the isolates. The aac (3)-IIa and aac(6ʹ)-Ib genes were found in 71% (n=24) and 5.8% (n=2) of aminoglycoside resistant isolates, respectively. Simultaneous carriage of aac (3)-IIa and aac(6ʹ)-Ib was detected in 64% (n=22) of the aminoglycoside resistant isolates.
Conclusions: The results of this study showed the presence of aac (3)-IIa genes in more than 70% of the aminoglycosides resistant K. pneumoniae strains; this may be due to the transmission of this gene through mobile genetic elements that create a high risk of rapid spread of these genes in hospitals.

Full-Text [PDF 422 kb]   (527 Downloads) |   |   Full-Text (HTML)  (400 Views)  
Type of Study: Research | Subject: Bactriology
Received: 2017/01/17 | Accepted: 2018/03/18 | Published: 2018/03/18

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | International Journal of Medical Laboratory

Designed & Developed by : Yektaweb