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Showing 3 results for Samadi
Serum levels of IL-17A increase in Asthma but not in accordance with Serum level of IgE and Asthma Severity
Masouma Mowahedi, Mohammad Samet, Fateme Zare, Morteza Samadi,
Volume 2, Issue 1 (May 2015)
Abstract

Background and Aims: Recent evidence suggests that T helper (Th) 17 cells are involved in the emergence of asthma. Th17 cells have a key role in inducing inflammation in asthmatic airways thus Interleukin (IL)-17A, the main cytokine of Th17, contributes to airways inflammation.

Materials and Methods: We evaluated the level of IL-17A and total immunoglobulin E (IgE) in sera of 100 asthmatic patients and 81 healthy controls by ELISA to determine how serum concentration of IL-17A is associated with asthma severity. We classified patients into three groups mild (n=28), moderate (n=33) and severe cases (n=39).

Results: Respectively, serum IL-17A and IgE concentrations were significantly higher in the asthmatic patients than the control group (p=0.026 and p<0.01). Mean of serum IL-17A and IgE values were 37.73 pg/ml and 39.02 IU/ml in the control group and 68.55 pg/ml and 295.87 IU/ml in the patients group. Nevertheless there were non-significant differences between the three groups of asthmatic patients. Respectively, mean of serum IL-17A and IgE values were 94.17 pg/ml and 255.07 IU/ml in the mild group, 71.29 pg/ml and 271.27 IU/ml in the moderate group, and 47.85 pg/ml and 345.97 IU/ml in the severe group. Moreover, there was no correlation between serum levels of IL-17A and IgE.

Conclusions: In this study we found that IL-17A, like IgE, rises in sera of asthmatic patients though in a different manner. IgE increases in serum consistent with disease severity though the increases of IL-17A in serum has an inverse relationship with IgE rising.


Significant Associations of the rs3104413 Single-nucleotide Polymorphism in the HLA Region with Type 1 Diabetes
Sepideh Jamehbozorg, Gilda Eslami, Ghasem Solgi, Hamzeh Rezaei, Mehrdad Hajilooi, Morteza Samadi,
Volume 5, Issue 1 (February 2018)
Abstract
Background and Aims: In this study, the effect of rs310441 polymorphism in the human leukocyte antigen (HLA) region on the development of susceptibility or resistance to Type 1 diabetes (T1D) among the people with T1D compared to healthy subjects has been investigated.
Materials and Methods: This research, which is based on the examination of 130 cases with T1D and 98 controls, has been carried out in the city of Hamedan after clinical examination. In order to determine the HLA gene polymorphism, the allele-specific-refractory mutation system-polymerase chain reaction (ARMS-PCR) method was utilized.
Results: This study indicated that there is a significant relationship between the frequency of alleles and genotypes in the patients compared to healthy subjects. The C/C and C/G genotypes were more frequent in patients than controls and G/G genotype was shown to be protective for T1D (p=0.01). Significant difference was found for the G allelic frequency in patients with T1D and in the control group. The allelic frequency was significantly different between the two groups (p=0.0001). Our findings indicate that HLA polymorphism(C/G) and (C/C) genotypes could be considered as genetic risk factors associated with susceptibility and (G/G) genotypes associated with protection for T1D.
Conclusions: This study identified that there is a significant relationship between the frequency of alleles and genotypes in the patients compared to healthy subjects.

An Investigation of the Relationship Between HLA-CW06 and Psoriasis in Iranian Patients Using Sequence-specific Primers
Zahra Shakeri Fini , Payam Kheirmand Parizi , Seyed-Alireza Esmaeili, Abbas Samadi-Khouzani , Sara Hosseini , Mostafa Jafarpour ,
Volume 9, Issue 2 (May 2022)
Abstract
Background and Aims: Psoriasis is a chronic inflammation of the skin caused by the proliferation of inappropriately differentiated horn cells, resulting in plaque psoriasis formation. It is not often fatal, and it may lead to physical disabilities and severe mental stress in which professional and social activities could be highly affected. It is generally accepted that the human leukocyte antigen (HLA)-CW06 allele has a significant role in the onset of this disease.
Materials and Methods: This study determines the association between the HLA-CW06 allele and psoriasis. The present case-control study was conducted to evaluate the relation between HLA-CW06 and psoriasis in a population in Iran. This study was performed on 30 samples of patients with psoriasis.
Results: The results of polymerase chain reaction- sequence-specific primers for the detection of HLA-CW06 showed positive amplification in 7 out of 30 psoriasis patients as compared to 4 among 30 controls.
Conclusion: This study showed that due to different allele frequencies associated with psoriasis in different parts of the world, it seems that other genetic and epigenetic factors may be involved in this disease.

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