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Showing 4 results for deyhim
Frequencies of HLA-E*01:01/*01:03 Alleles in an Iranian Healthy Population and Its Comparison with Hyperglycemia Patients
Zahra Hatami, Ahmad Zavaran Hossieni, Fatemeh Yari, Mohammad Reza Deyhim,
Volume 4, Issue 4 (November 2017)
Abstract
Background and Aims: The human leukocyte antigen-E (HLA-E) is a member of non-classical HLA class-I genes which is highly conserved through evolution. In general, so far 25 alleles of HLA-E have been described. However, the existence of only two allelic group; HLA-E*01:01 and HLA-E*01:03 have been demonstrated in all the populations. HLA-E*01:01 and HLA-E*01:03 differ only at codon 107 of exon 3. The aim of this study was to survey on the frequency of HLA-E alleles; HLA-E*01:01 and HLA-E*01:03 in healthy and high blood sugar subjects.
Materials and Methods: Genomic DNA was extracted from the whole blood of 137 randomly selected healthy individuals and 86 patients with high blood sugar by using salting-out method. HLA-E*01:01 and HLA-E*01:03 alleles were determined by polymerase chain reaction sequence-specific primers. To overcome the ambiguity of some results, polymerase chain reaction-restriction fragment length polymorphism method saw used. Finally, the nucleotide sequence of a limited number of polymerase chain reaction products was determined.
Results: In healthy subjects, the allelic frequency of HLA-E*01:01 and HLA-E*01:03 were 48.9% and 51.1%, respectively. In high blood sugar subjects, the allelic frequency of HLA-E*01:01 and HLA-E*01:03 were 50.6% and 49.4%, respectively. The dominant allele in the healthy subjects was HLA-E*01:03 and in high blood sugar group was HLA-E*01:01 without significant differences for HLA-E*01:01 and HLA-E*01:03 frequencies in high blood sugar group.
Conclusions: Our results about the frequencies of HLA-E*01:01 and HLA-E*01:03 alleles in Iranian population showed similarities with French and Korean populations.
Capability of Platelet Factor 4 to Induce Apoptosis in the Cancerous Cell Lines in Vitro
Alireza Goodarzi, Fatemeh Yari, Mahshid Mohammadipour, Mohammad Reza Deyhim, Hossin Timori Naghadeh,
Volume 5, Issue 3 (August 2018)
Abstract
Background and Aims: Platelet factor 4 (PF4) or CXCL4 is a member of CXC chemokine family which is stored in alpha granules of platelets. The main function known for PF4 is angiostasis which may contribute to prevent tumor metastasis. This feature is mediated by CXCR3 on the endothelial cells. Our principal aim was to study the apoptosis induction in three cell lines treated with PF4 and obtained from human platelet concentrates.
Materials and Methods: We evaluated the apoptotic effect of platelet-derived PF4 on the U266B1 and K562 cell lines expressing CXCR3, compared with Daudi as a CXCR3-negative cell line. PF4 was purified from human platelet concentrates by immunoaffinity chromatography and was concentrated. The quantity and molecular weight of the obtained PF4 was determined by enzyme-linked immunosorbent assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis methods respectively. Cell lines were treated for 72 and 96 h with 90 μg/ml of PF4. Apoptosis was assayed by using CD95, WST-1, active caspase-3, and cell count.
Results: Platelet-derived PF4 was a weak agent to induce apoptosis in U266B1 and K562 cell lines. Our data showed in terms of WST-1 and cell count had a significant difference between control and experiment groups (p≤0.05), while CD95, LDH, and active caspase-3 did not show such a difference (p>0.05).
Conclusions: We observed that PF4 released from platelets has a weak potential to induce apoptosis in cancerous cell lines. Other factors may also contribute to this process including the applied dose, purification method, cell line type, and its proteoglycan carrier.

Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
Shaghayegh Pishkhan Dibazar, Ahmad Zavaran Hosseini, Fatemeh Yari, Shirin Fateh, Mohammad Reza Deyhim,
Volume 5, Issue 4 (November 2018)
Abstract
Background and Aims: Ficolins are proteins that bind to carbohydrates, act as opsonins and play an important role in innate immunity. Polymorphism in ficolin-3 gene (FCN3) can lead to complement deficiency and increase the risk of some disorders such as diabetes. The aim of this study was to investigate the frequency of FCN3+1637delC as a single nucleotide polymorphism in this gene in healthy and diabetic subjects of Iran.
Materials and Methods: Blood was taken from 36 diabetics and 37 healthy subjects who had referred to the Iranian Blood Transfusion Organization. Blood sugar was analyzed using a calorimetric method. After DNA extraction using salting out method, polymerase chain reaction (PCR) was carried out and the restriction fragment length polymorphism (RFLP) method was accomplished using ApaI restriction enzyme. Consequently, the resulted fragments were evaluated using electrophoresis on 2% L-agarose gel.
Results: Evaluation of the results indicated that the heterozygote form of single-nucleotide polymorphism FCN3+1637delC was seen in three samples (8.1%) of the studied healthy subjects and in two samples (5.6%) of the diabetic individuals. Besides, the homozygous form of the mutation was not seen in the studied healthy and diabetic subjects.
Conclusions: Results of this study showed that FCN3 variant of single-nucleotide polymorphism FCN3+1637delC was not considered as a risk factor for type 2 diabetes mellitus in Iranian subjects.

Evaluation of Lipid Peroxidation, Nitric Oxide Metabolites, and Plasma Total Homocysteine Concentration in Patients with Chronic Kidney Disease
Zeynab Ahmarinezhad, Mohammad Reza Deyhim, Mahbub Lesan Pezeshki, Mohammad Taghi Najafi, Fahimeh Khoshnaghsh,
Volume 11, Issue 2 (May 2024)
Abstract
Introduction: Numerous factors contribute to the advancement of progressive kidney damage in chronic kidney disease (CKD). Among these, oxidative damage plays a significant role in the progression of CKD. The reactions involving free radicals are recognized as a crucial element that can exacerbate oxidative damage in patients with CKD. However, the precise mechanisms underlying oxidative damage remain incompletely understood. Consequently, this study aimed to explore oxidative and other associated biochemical parameters.
Materials and Methods: This case-control study included 38 male and 23 female patients, with mean ages of 58.9 ± 15.9 and 62.13 ± 13.43 years, respectively. At the same time, 40 healthy male and 22 healthy female individuals with an average age of 60.33 ± 10.62 and 59.3 ± 6.64, respectively, were selected as the control group who had no history of any diseases such as hypertension, diabetes, infection and kidney disease. Research variables including malondialdehyde (MDA), nitric oxide metabolites (nitrite/nitrate), and homocysteine (Hcy) concentration were measured in both case and control groups. Statistical analysis was done according to the obtained results.
Results: There was a significant increase in homocysteine concentration in CKD patients compared to the control (P = 0.001). Nitrite and nitrate metabolites exhibited a substantial reduction in patients when compared to the control group (P = 0.001). On the other hand, malondialdehyde (MDA) showed a significant increase in patients compared to controls (P = 0.01).
Conclusion: The findings indicate that homocysteine levels, lipid peroxidation, and alterations in nitric oxide may significantly contribute to the progression of oxidative damage in patients with chronic kidney disease (CKD) and may also influence the disease’s pathogenesis.

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