Showing 4 results for Hbsag
Zakieh Rostamzadeh , Mohadese Nemati , Mahshid Mohammadian ,
Volume 4, Issue 2 (5-2017)
Abstract
Background and Aims: Hepatitis B virus (HBV) infection is a serious global health problem affects many people. Recently, because of vaccination, the incidence of HBV infection has been reduced, but in high risk population also health care students (HCS) adequate immunization is serious. The aim of this survey was to evaluate HBV immunization in the population of the paramedical students based on the demographic characteristics in Iran.
Materials and Methods: Anti-HBsAg titer was prospectively assayed in all vaccinated of the 95 HCS using Enzyme-linked immunosorbent assay.
Results and Conclusions: Anti-HBsAg titer in the studied population was estimated about 92.8±80.5 mIU. Anti-HBsAg titer response was significantly higher in females to males. A low proportion of vaccinated HCS had low titers antibody against HBV infection. Therefore, measuring anti-HBsAg titer may help to drop in HBV incidence in HCS. This reduction can be correlated with the effectiveness of national immunization program.
Seyed Javad Pourafzali, Azad Khaledi, Rahele Miri, Seyed Ali Akbar Shamsian, Seyed Abdolrahim Rezaee, Ahmad Piroozmand, Kiarash Ghazvini,
Volume 5, Issue 3 (8-2018)
Abstract
Background and Aims: Almost 350 million people worldwide are carriers of hepatitis B virus. The rate of chronic carriers in Iran is about 3%. Therefore Iran is categorized as a country with moderate rate of infection with this virus. In developing countries, transmission from mother to fetus and newborn is considered as one of the main routes of transmission. So, this study investigated the prevalence of hepatitis B and its risk factors in pregnant women.
Material and Methods: In this descriptive cross-sectional study which has been done in 2010-2011, of 759 pregnant women referred to the Central Laboratory of ACCER and laboratory of Mashhad Ghaem hospital for HBsAg testing, after obtaining consent form, patients entered to the study with simple non-probability sampling, and a questionnaire was completed about demographic characteristics and related factors. Then data were analyzed by SPSS ver.13.
Results: The mean age of participants was 28.16 ± 4.8, the prevalence of HBsAg among pregnant women was reported 0.3%, and also because of finding only two cases of HBsAg positive, significant association was not found with socio-demographic factors such as; vaccination history of hepatitis B (P= 0.527) and risk factors including family history of hepatitis B or hepatitis C (P= 0.999).
Conclusions: Our study revealed that the prevalence rate of HBsAg positive among pregnant women is low. The prevalence of obtained HBsAg in this study compared to other studies from Iran is low, so, further studies with more cases are necessary to achieve real estimation of HBsAg positive among pregnant women.
Rana Tabar Asad Laleh, Zohreh Sharifi, Ali Akbar Pourfathollah, Shahram Samei,
Volume 6, Issue 1 (2-2019)
Abstract
Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province.
Materials and Methods: The present cross-sectional experiment was conducted on 3500 voluntary blood donors in Golestan province to examine the presence of human immunodeficiency viruses Ag-Ab, HBsAg, and hepatitis C virus Ab. Then, samples with negative results for the mentioned tests were screened for total HBc antibody (IgM-IgG) through ELISA technique. Afterward, HBV-DNA extraction and R-T PCR assay were conducted for all HBsAg negative samples by using Real ART HBV LC PCR kit on a Light Cycler instrument.
Results: The study participants included 3255 (93%) male and 245 (7%) female. In general, 385 (11%) out of 3500 samples were anti-HBc positive. HBV-DNA results for every sample with either positive or negative anti-HBc were found to be negative.
Conclusions: As the area under study has a high rate of anti-HBc outbreak (11%) without the presence of HBV-DNA, anti-HBc screening can cause blood donor deferrals and limit blood supply; therefore, the HBsAg test with high analytical sensitivity is recommended for HBV screening in this area. Regarding the cost analyses and also the status of HBV endemicity, HBsAg test along with ID-NAT is preferable, if possible, for improving blood safety.
Hussein Khani, Mahdi Ghorbani, Farshad Nojoomi, Alireza Mohebbi,
Volume 6, Issue 4 (11-2019)
Abstract
Background and Aims: Currently, many efforts are directed toward functional Hepatitis B virus (HBV) treatment. This is achievable by suppression of HBV surface antigen (HBsAg) secretion. In this regard, use of natural products has been the areas of interest by scientific communities.
Materials and Methods: Dried Honey Bee venom was extracted for assessing its anti-HBsAg secretion potential. Hepatoma cell-derived PLC/PRF/5 was propagated in complete medium. The cell line was treated by a serial dilution of Bee venom. Cell cytotoxicity (IC50) was measured by MTT colorimetric assay at three post-treatment times. HBsAg secretion was evaluated from PLC/PRF/5 supernatant treated by under-cytotoxic concentrations of Bee venom by using enzyme-linked immunosorbent assay.
Results: The results indicated that dried Bee venom extract is able to reduce secretion of HBsAg from the cell line with Selectivity Index (SI) of eight. Reduced levels of HBsAg were in dose-dependent manner and it was in its lower concentrations at 8 ppm after 12 hr post treatment. The IC50 was observed to be 63.78 ppm.
Conclusions: The Bee venom has anti-HBV activity. The way we used under-cytotoxic concentration of Bee venom, the HBsAg secretion was restored after 24 hr post treatment. Furthermore, mechanism of action of Bee venom in reducing HBsAg level needs to be further investigated.
