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Showing 2 results for Hla-E
Frequencies of HLA-E*01:01/*01:03 Alleles in an Iranian Healthy Population and Its Comparison with Hyperglycemia Patients
Zahra Hatami, Ahmad Zavaran Hossieni, Fatemeh Yari, Mohammad Reza Deyhim,
Volume 4, Issue 4 (11-2017)
Abstract
Background and Aims: The human leukocyte antigen-E (HLA-E) is a member of non-classical HLA class-I genes which is highly conserved through evolution. In general, so far 25 alleles of HLA-E have been described. However, the existence of only two allelic group; HLA-E*01:01 and HLA-E*01:03 have been demonstrated in all the populations. HLA-E*01:01 and HLA-E*01:03 differ only at codon 107 of exon 3. The aim of this study was to survey on the frequency of HLA-E alleles; HLA-E*01:01 and HLA-E*01:03 in healthy and high blood sugar subjects.
Materials and Methods: Genomic DNA was extracted from the whole blood of 137 randomly selected healthy individuals and 86 patients with high blood sugar by using salting-out method. HLA-E*01:01 and HLA-E*01:03 alleles were determined by polymerase chain reaction sequence-specific primers. To overcome the ambiguity of some results, polymerase chain reaction-restriction fragment length polymorphism method saw used. Finally, the nucleotide sequence of a limited number of polymerase chain reaction products was determined.
Results: In healthy subjects, the allelic frequency of HLA-E*01:01 and HLA-E*01:03 were 48.9% and 51.1%, respectively. In high blood sugar subjects, the allelic frequency of HLA-E*01:01 and HLA-E*01:03 were 50.6% and 49.4%, respectively. The dominant allele in the healthy subjects was HLA-E*01:03 and in high blood sugar group was HLA-E*01:01 without significant differences for HLA-E*01:01 and HLA-E*01:03 frequencies in high blood sugar group.
Conclusions: Our results about the frequencies of HLA-E*01:01 and HLA-E*01:03 alleles in Iranian population showed similarities with French and Korean populations.
Correlation of Tissue Distribution of HLA-G and HLA-E and Degree of Tumor Malignancy in Patients with Breast Cancer
Mahdie Jaafari , Hossein Hadinedoushan, Mahmoud Akhavan Tafti ,
Volume 9, Issue 4 (11-2022)
Abstract
Background and Aims: Human leukocyte antigen (HLA) molecules play a substantial role in T Lymphocyte-mediated adaptive immune response. Down-regulation of HLA expression may help the tumor to escape from immune surveillance. This study aimed to evaluate the correlation between HLA-G and HLA-E tissue distribution and the degree of tumor malignancy in human breast tumors.
Materials and Methods: This cross-sectional study was conducted
on tissue samples of 145 patients with breast cancer. The distribution of HLA-G and HLA-E expressions were measured by the immunohistochemistry method.
Results: Among 145 patients, 51% of tumors did not express HLA-E, and +1 positive was seen in 36.6 % of patients. +2 positive in 8.3% and +3 positive in 4.1% of patients. Moreover, 79.3% of tumors did not express HLA-G, 17.9% expressed +1 positive, 2.8% expressed +2 positive, and no patients expressed +3 positive. Generally, 20.7% and 49% of patients showed expression of HLA-G and HLA-E, respectively. A significant correlation was seen between the grade of disease and expression of HLA-E (p = 0.03) and HLA-G (p = 0.001). Moreover, a significant correlation was seen between the simultaneous expression of HLA-G and HLA-E with grade (p = 0.03, r = 0.176).
Conclusion: Significant correlation was seen between the distribution of HLA-G and HLA-E expression with a degree of malignancy. Therefore, these biomarkers’ expression may contribute to the prognosis and progression of breast cancer.

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