en باکتری شناسی Bactriology پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims</strong>: Group B <em>streptococcus</em> (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, therefor this investigation was aimed to determine the surface proteins genotype of detected GBS from both vagina and urine of pregnant women.<br> <strong>Materials and Methods:</strong> In the present study, a total of 346 vaginal and urine samples were obtained from the same pregnant women. Following culturing the samples on sheep blood agar medium and related tests (CAMP, Hiporate hydrolysis) the suspected colonies were further confirmed by polymerase chain reaction (PCR) technique. The detected GBS species were then genotyped using multiplex PCR assay.<br> <strong>Results:</strong> Out of 346 specimens, 57 (16.47%), and 33 isolates (9.5%) were identified as vaginal and urine GBS, respectively. Genotype <em>rib</em> was predominant in both vaginal discharge and urine specimens, by frequencies of 56.1% and 54.5%, respectively. Other genotypes of vaginal GBS were as <em>epsilon</em> (36.8%), <em>anlpha-C</em> (3.5%), and <em>alp2/3</em> (3.5%); while GBS in urine were as <em>epsilon</em> (36.4%), <em>alp2/3</em> (6.1%), and <em>anlpha-C</em> (3%).<br> <strong>Conclusion<a name="_GoBack"></a>s:</strong> The prevalence of GBS in both vaginal and urine samples were reliable in comparison to other societies. These results showed that genotype <em>rib</em> of GBS, which seems to be associated to neonatal diseases, is dominant genotype in both urine and vaginal samples.</div> Genotyping , Multiplex PCR, Pregnant women, Streptococcus agalactiae 180 188 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-191-1&slc_lang=en&sid=1 Anahita Nazari آناهیتا نظری نظری No Department of Microbiology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohamad Bagher Khalili محمد باقر خلیلی khalilimb96@gmail.com Yes Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. دپارتمان میکروبیولوژی، دانشکده پزشکی، داشنگاهه علو پزشکی شهید صدوقی، یزد Akram Astani اکرم آستانی khalilimb96@gmail.com No Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran دپارتمان میکروبیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید صدوقی، یزد Mahmood Vakili محمود وکیلی khalilimb96@gmail.com No Department of Public Medicine, Faculty of medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran دپارتمان پزشکی عمومی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید صدوقی، یزد Maryam Sadeh مریم ساده khalilimb96@gmail.com No Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mahdiye Mojibiyan مهدیه مجیبیان khalilimb96@gmail.com No Obstetrics and Gynecology Department, Mojibiyan Hospital, Yazd, Iran Shahla Rashighi شهلا رشیقی khalilimb96@gmail.com No Obstetrics and Gynecology, Seyedoshohada Clinic, Yazd, Iran Fariba Reyhanizadeh فریبا ریحانی زاده khalilimb96@gmail.com No BSc of Midwifery, Baghayipour Clinic, Yazd, Iran