fa درصد لنفوسیت های Th17 در خون محیطی بیماران ایرانی مبتلا به فرم اتوزومال مغلوب سندرم هایپر IgE ایمنی شناسی Immunology پژوهشي Research <div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong><span style="font-family:b zar;"><span style="font-size:12.0pt;"> بیماران مبتلا به سندرم هایپر </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IgE</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> از عفونت های باکتریایی و قارچی به خصوص استافیلوکوک اورئوس و کاندیدا آلبیکنس رنج میبرند. با توجه به نقش مهم لنفوسیت های&nbsp; </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> در دفاع از عفونت های قارچی، درصد لنفوسیت‌های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> در بیماران مبتلا به سندرم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">hyper-IgE</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> اتوزومی مغلوب (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">AR-HIES</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">) مورد بررسی قرار گرفت.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:b zar;"><span style="font-size:12.0pt;">مواد و روشها:</span></span></strong><span style="font-family:b zar;"><span style="font-size:12.0pt;"> در این مطالعه مورد-شاهدی، 6 بیمار مبتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">AR-HIES</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> (با جهش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">DOCK-8</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">) و هفت کنترل سالم که از نظر سن و جنس همسان سازی شده بودند، مورد مطالعه قرار گرفتند. سلول های تک هسته ای خون محیطی از خون وریدی بیماران جدا شد و درصد لنفوسیت های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> توسط روش فلوسایتومتری سنجش شد.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:b zar;"><span style="font-size:12.0pt;">نتایج:</span></span></strong><span style="font-family:b zar;"><span style="font-size:12.0pt;"> از لحاظ آماری تفاوت معنی داری بین درصد لنفوسیت های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> در گروه مورد و شاهد وجود نداشت (15/0=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">). همچنین در مقایسه با افراد گروه کنترل، تعداد ائوزینوفیل‌ها به طور چشمگیری افزایش نشان داد. (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p=0.000</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">). از طرفی همبستگی معنی دار منفی میان سطح سرمی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IgE</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> و درصد لنفوسیت‌های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> وجود داشت (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">r=-0.927</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p=0.006</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">). همچنین یک همبستگی مثبت بین تعداد ائوزینوفیل ها و درصد لنفوسیت‌های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> وجود داشت (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">r=0.557</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p=0.01</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">). سطح سرمی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IgE</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> همبستگی معنی دار مثبتی با تعداد ائوزینوفیل ها در خون محیطی بیماران نشان داد (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">r=0.961</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p=0.003</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">).</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:b zar;"><span style="font-size:12.0pt;">بحث و نتیجه گیری:</span></span></strong><span style="font-family:b zar;"><span style="font-size:12.0pt;"> احتمالا میان درصد لنفوسیت های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">Th17</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> در بیماران </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">AR-HIES</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> و گروه کنترل اختلاف معنی داری وجود ندارد. تعداد ائوزینوفیل ها در بیماران </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">AR-HIES</span></span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;"> به میزان قابل توجهی نسبت به گروه کنترل افزایش نشان داد</span></span><span style="font-family:b zar;"><span style="font-size:12.0pt;">.</span></span></div> <p style="text-align: justify;"><strong>Background and Aims:</strong> Patients with Hyper-IgE syndrome suffer from fungal and bacterial infections, especially Candida albicans and Staphylococcus aureus. Due to the important role of T helper17 (Th17) lymphocytes in defense against fungal infections, the percentage of Th17 lymphocytes was studied in the patients with autosomal recessive hyper-IgE syndrome (AR-HIES).</p> <p style="text-align: justify;"><strong>Materials and Methods:</strong> In this case-control study, six patients with AR-HIES (with DOCK-8 mutation) and seven healthy age and sex-matched controls were included. Peripheral blood mononuclear cells were isolated from their venous blood and the percentage of Th17 lymphocytes were determined by flow cytometry.</p> <p style="text-align: justify;"><strong>Results:</strong> There was no statistical difference between the percentage of Th17 lymphocytes (p=0.15) in the case and control groups. Also in comparison to the control subjects, the numbers of eosinophils were dramatically increased (p=0.000). Also, there was a significant negative correlation between serum IgE levels and Th17 lymphocytes&rsquo; percentage (r=-0.927, p=0.006) and a significant positive correlation between eosinophils number and Th17 lymphocytes&rsquo; percentage (r=0.557, p=0.01). Serum IgE levels showed a significant positive correlation with the numbers of eosinophils in the patients&rsquo; peripheral blood with AR-HIES (r=0.961, p=0.003).</p> <p style="text-align: justify;"><strong>Conclusions:</strong> The numbers of Th17 in the patients with AR-HIES may not show statistical differences between the cases and controls. The numbers of eosinophils significantly increased in the patients AR-HIES compare to the controls.</p> AR-HIES , سندرم هایپر IgE , Th17 AR-HIES, Hyper IgE Syndrome, Th17 101 110 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-1-49&slc_lang=fa&sid=1 Arezou Rahimi No Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mehrnaz Mesdaghi Yes Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Nima Rezaei No Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mahboubeh Mansouri No Department of Allergy and Clinical Immunology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Iraj Mohammadzadeh No Non-Communicable Diseases Research Center, Babol University of Medical Sciences, Babol, Iran. Hamid Farajifard No Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mehrdad Amirmoini No Department of Allergy and Clinical Immunology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Delara Babaie No Department of Allergy and Clinical Immunology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Reza Alimohammadi No Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Zahra Chavoshzadeh No Department of Allergy and Clinical Immunology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.