en Antibiotic-resistance determinants in Acinetobacter baumannii strains isolated from burn patients in Iran باکتری شناسی Bactriology پژوهشي Research &nbsp;<br> <strong>Background</strong> <em>Acinetobacter baumannii</em> is an important multidrug-resistant opportunistic pathogen that frequently causing various nosocomial infections and is a serious threat to burn patients. These infections usually caused by the outbreak strains.<br> <strong>Objective:</strong> The aim of this study was to show antibiotic resistance pattern and molecular typing of <em>A.baumannii</em> genes isolates collected from burn patients.<br> <strong>Methods:</strong> In this study, 307 different strains were detected. 100 <em>Acinetobacter baumannii</em> strain was selected in burn center in Isfahan hospital. Antibiotic resistance pattern was determined by disk diffusion method (Kirby Bauer). The presence of genes coding in antibiotic resistance were analyzed by using of M-PCR method.<br> <strong>Results: </strong>The antibiotic resistance pattern for <em>Acinetobacter baumannii</em> show the high resistance for ciprofloxacin, ceftazidime, and tetracycline with frequency of 82.5%, 75.3%, and 72%, respectively. Moreover, the most sensitive antibiotics were chloramphenicol, and nitrofurantoin with frequency of 3.9% and 2.8% resistance. CITM (91.1%) was the highest detected genes.<br> <strong>Conclusions:</strong> High prevalence of antibiotic resistance pattern among <em>Acinetobacter baumannii</em> isolated from burn center hospitals indicate the importance role of multidrug resistant isolates.<br> <br> &nbsp; <div style="text-align: justify;"><strong>Background and Aims:</strong> <em>Acinetobacter baumannii</em> (<em>A.&nbsp;baumannii</em>) is an important multidrug-resistant opportunistic pathogen frequently causing various nosocomial infections and is a serious threat to burn patients. These infections are usually caused by the outbreak strains. The aim of this study was to show antibiotic resistance pattern and molecular typing of <em>A.&nbsp;baumannii </em>genes isolates collected from burn patients and also distribution of different types of burn patients.<br> <strong>Materials and Methods:</strong> In this study, 307 different strains were detected. Totally 100 <em>A.&nbsp;baumannii </em>strain was selected in burn center of Isfahan hospital. Antibiotic resistance pattern was determined by disk diffusion method (Kirby Bauer). The presence of genes coding in antibiotic resistance were analyzed by using multiplex-PCR method. The standard strains of <em>Escherichia coli</em> ATCC 25922 and <em>A.&nbsp;baumannii </em>ATCC 19606 were used as negative and positive controls.<br> <strong>Results: </strong>The antibiotic resistance pattern for <em>A.&nbsp;baumannii </em>showed high resistance for ciprofloxacin, ceftazidime, and tetracycline with frequency of 82.5%, 75.3%, 72%, respectively. Moreover, the most sensitive antibiotics were chloramphenicol, and nitrofurantoin with the resistance frequency of 3.9% and 2.8%. <em>CITM </em>(91.1%) was the highest detected gene.<br> <strong>Conclusions:</strong> High prevalence of antibiotic resistance pattern among <em>A.&nbsp;baumannii </em>isolated from burn center hospitals indicates the important role of multidrug resistant isolates.&nbsp;</div> Acinetobacter baumannii, multidrug-resistant, burns, Multiplex PCR Acinetobacter baumannii, Burn units, Multiplex PCR, Multidrug-Resistant 278 287 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-68-4&slc_lang=en&sid=1 Fahimeh Nourbakhsh فهیمه نوربخش nourbakhshf951@mums.ac.ir Yes Department of Pharmacodynamics and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. دانشگاه علوم پزشکی مشهد Elaheh Tajbakhsh الهه تاج بخش ee_tajbakhsh@yahoo.com No 2. Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. دانشگاه ازاد اسلامی شهرکرد Dana Daneshmand دانا دانشمند fahimeh_nourbakhsh@yahoo.com No 3. Nosocomial Infection Research Center, Isfahan University of Medical Science, Isfahan, Iran مرکز تحقیقا عفونی دانشگاه اصفهان Samaneh Borooni سمانه برونی s.borooni@yahoo.com No 4. Nourdanesh Institute of Higher Education, Meymeh, Esfahan, Iran مرکز غیر انتفاعی میمه Vajiheh Nourbakhsh No Hazrat Fatemeh Zahra Hospital, Affiliated to the Treatment and Management of Social Security in Isfahan, Isfahan, Iran