en انگل شناسی Parasitology پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims:</strong> <em>Trichomonas vaginalis</em> is a flagellated protozoa that is associated with vaginitis, cervicitis, urethritis and other vaginal disorders. Current study aimed to evaluate the anti-Trichomonas activity of <em>Medicago sativa</em> and <em>Satureja hortensis</em>, <em>in vitro</em>.<br> <strong>Materials and Methods: </strong>Ethanolic extract of <em>Medicago sativa</em> and <em>Satureja hortensis</em> were obtained by rotary evaporator. anti-Trichomonas vaginalis activities of the extracts in different concentrations were evaluated after 24, 48 and 72 hr of incubation of the cultured media.<br> <strong>Results and Conclusions: </strong>The data showed a significant difference between concentration and time regarding the <em>Satureja hortensis</em> and <em>Medicago sativa</em> extracts compared to the negative control (p<0.05). According to the results, the anti- trichomonas activity of the <em>Medicago sativa</em> and <em>Satureja hortensis</em> extracts may make it possible to use them in the treatment of trichomoniasis.</div> Satureja hortensis, Medicago sativa, Trichomonas vaginalis, invitro Medicago sativa, Satureja hortensis, Trichomonas vaginalis 166 171 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-264-1&slc_lang=en&sid=1 Farzaneh Mirzaei فرزانه میرزایی mirzaei.farzaneh@yahoo.com 0000-0002-2788-0146 No Department of Parasitology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Vahid Raissi وحید رئیسی vahidraissi66@gmail.com 0000-0003-0504-2982 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran Aref Teimouri عارف تیموری No Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran Parisa Mousavi پریسا موسوی No Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran Mohammad Ali Mohaghegh محمدعلی محقق mohaghegh1982@yahoo.com 0000 0001 8301 5256 No Department of Laboratory Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. Mahdi Dehghan-manshadi مهدی دهقان منشادی 0000-0003-0145-244X No Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Fateme zare فاطمه زارع No Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Bahman Rahimi Esboei بهمن رحیمی اسبوی bahman51640@yahoo.com 0000-0003-2595-9907 Yes Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran.