en تمایز سلولهای بنیادی بالغ به غضروف با استفاده از داربست زیستی مهندسی بافت غضروف ژنتیک/ بیوتکنولوژی Genetics/ Biotechnology پژوهشي Research <div style="text-align: justify;"><strong>زمینه و هدف: </strong>غضروف یک بافت بسیار تخصصی است که قابلیت ترمیم خود را&nbsp;در مواقع آسیب ندارد.&nbsp; <span title="Although the invention of the method of surgery with autologous chondrocyte transplantation, developed tools to treat the cartilage lesions, it couldn’t gain a great success due to problems such as damage to the area of donation.">اگر چه روش</span>&shy;های جراحی با پیوند کندروسیت اتولوگ، روش&shy;هایی توسعه یافته برای درمان ضایعات غضروفی هستند، اما نمی توانند در مواجهه با مشکلات ناشی از آسیب ناحیه پیوندی موفقیت&shy;های بزرگی حاصل نمایند<span dir="LTR">. &nbsp;</span><span title="With revenues in the field of tissue engineering and sciences using mesenchymal stem cells derived from adipose and culturing and differentiation of them on suitable scaffolds was considered appropriate as a successful research and clinical strategy. ">با پیشرفت در زمینه مهندسی بافت و دانش استفاده از سلول های بنیادی مزانشیمی مشتق از چربی و کشت و تمایز آنها در داربست های مناسب </span>به عنوان استراتژی موفق بالینی در نظر گرفته میشوند.<br> <strong>مواد و روشها: </strong>در این مطالعه پس از آماده سازی دو داربست چسب فیبرینی و آلژینات، سلول های بنیادی مزانشیمی جدا شده از بافت چربی جداگانه بر روی این دو داربست قرار گرفتند و در محیط کندروژنی کشت داده شدند<span dir="LTR">.&nbsp;</span><span dir="RTL"> 1، 7 و 14 روز پس از تمایز سلولی، توانایی زنده ماندن سلول های تمایز یافته توسط</span> <span dir="LTR">MTT</span> کندروژنی مورد تجزیه و تحلیل قرار گرفت<span dir="LTR">. </span><span title="Also, The expression of collagen type I, type II, sox9 and aggrecan were assessed by Real time, PCR in 0, 1, 3, 7 and 14 days after cell differentiation.">همچنین بیان کلاژن نوع</span> <span dir="LTR">I</span>،نوع <span dir="LTR">II</span> و <span dir="LTR">SOX9</span> و اگریکان به روش <span dir="LTR">Real time PCr</span> مورد بررسی قرار گرفت. همچنین تشکیل غضروف ها نیز به لحاظ بافت شناسی مورد ارزیابی قرار گرفت.<br> <span title="Results: According to the results taken from this study, the fibrin glue scaffold had a meaningful difference with Alginate scaffold in differentiated cells."><strong>یافته ها:</strong> بر اساس نتایج حاصل از این مطالعه، داربست</span><span title="Results: According to the results taken from this study, the fibrin glue scaffold had a meaningful difference with Alginate scaffold in differentiated cells."> چسب</span><span title="Results: According to the results taken from this study, the fibrin glue scaffold had a meaningful difference with Alginate scaffold in differentiated cells."> فیبرینی&nbsp; با داربست آلژینات در سلول های تمایزی اختلاف معنی داری داشت</span><span dir="LTR">. </span><span title="According to MTT analysis and in addition fibrin glue had the highest expression in chondrogenic gens among other scaffolds. ">با توجه به تجزیه و تحلیل</span> <span dir="LTR">MTT</span> و علاوه بر این، چسب فیبرینی بالاترین بیان در ژن های کندروژنیک را در میان دیگر داربست ها بود<span dir="LTR">.</span><br> <strong>نتیجه گیری:</strong> با توجه به نتایج این مطالعه میتوان چنین عنوان نمود که استفاده از داربست های طبیعی مانند چسب فیبرینی می&shy;تواند همانند پروتئین های مزانشیمی سلول های بنیادی به عنوان استراتژی کارآمد و جدید در مهندسی بافت و ترمیم عمل نماید.<br> &nbsp;</div> <div style="text-align: justify;"><strong>Background and Aims:</strong> Cartilage is a very specific tissue, which does not have the capacity to heal and renew itself<span dir="RTL">.</span> Although the invention of the method of surgery with autologous chondrocyte transplantation, developed tools to treat the cartilage lesions, it couldn&rsquo;t gain a great success due to problems such as damage to the area of donation. Using the mesenchymal stem cells derived from adipose and culturing and differentiating them on scaffolds was considered appropriate as a successful research and clinical strategy<em>.</em><br> <strong>Materials and Methods:</strong> In the present study, the mesenchymal stem cells were separated from adipose tissue and cultured in two scaffolds of fibrin glue and alginate medium. After 1, 7 and 14 days of cell differentiation, the survival ability of the differentiated cells were analyzed by Chondrogenic MTT. Moreover, type I and II collagen, aggrecan and Sox9 expression were measured via real time-polymerase chain reaction. In addition, cartilage reconstruction on scaffolds was shown by a histological investigation.<br> <strong>Results:</strong> Our results showed that the expression of CD90 and CD105 as mesenchymal markers is at a high level whereas the expression of CD34 and CD45 reaches a low level. The LSD test demonstrated that there was no remarkable difference among the chondrogenic MTT, scaffolds groups and control in 7 and 14 days after cell differentiation (p<0.05), although, fibrin glue had the highest expression in chondrogenic gens.<br> <strong>Conclusions:</strong> Finding suggests that in order to utilize a new strategy for tissue regeneration utilization of inherent scaffolds such as fibrin glue can act as a protector for mesenchymal stem cells.</div> سلول های بنیادی مزانشیمی , بافت چربی , چسب فیبرینی , آلژینات Adipose tissue, Alginates , Fibrin tissue adhesive, Mesenchymal stem cell 207 218 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-278-1&slc_lang=en&sid=1 Mahdieh Ghiasi مهدیه قیاثی mahdieh.ghiasi@yahoo.com No Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. Mohammad Mehdizadeh محمد مهدی زاده No Dental and Oral Research Center, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Qom University of Medical Sciences, Qom, Iran Ali Mohammadsharifi علی محمدشریفی sharifal@yahoo.com Yes Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. Gholamabbas Nasr غلامعباس نصر Abb13404@gmail.com No Eurasia University of Science and Research, International Campus, Tehran, Iran Mohammad Bigdelo محمد بیگدلو Jhandark2010@yahoo.com No Department of Surgery, Qom University of Medical Sciences, Qom, Iran. Sahar Farzaneh سحر فرزانه Sahar.farzaneh@gmail.com No Department of Biology, Islamic Azad University, Parand Branch, Parand, Iran. Farzaneh Tafvizi فرزانه تفویضی No Department of Biology, Islamic Azad University, Parand Branch, Parand, Iran. Sedigheh Safari صدیقه صفری No Department of Biology, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran . Neda Tekiyeh Maroof ندا تکیه معروف No Department of Molecular Biology, Islamic Azad Medical University of Tehran, Tehran, Iran.