en انگل شناسی Parasitology پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims:</strong> The protozoan parasites of the genus <em>Leishmania </em>are the causative agents of various clinical diseases. Different methods of cultivation of <em>Leishmania</em> parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of <em>Leishmania major.</em><br> <strong>Materials and Methods:</strong> Conventional LB broth medium was prepared and autoclaved for 15 min at 121&deg;C and then lyophilized rabbit anti-sheep cell haemolysin was added at 1-10% final concentrations. The efficacy of the medium was evaluated by assessing the growth ability and replication patterns of the promastigotes of <em>Leishmania major</em>.<br> <strong>Results:</strong> Medium with 1-10% lyophilized rabbit haemolysin supported the growth of the parasites and can be used for cultivation of <em>Leishmania</em> parasites with acceptable <em>In vivo</em> infectivity for research purpose.<br> <strong>Conclusions:</strong> The ability of the parasites to survive and proliferate in the presence of lyophilized rabbit haemolysin indicates that this material is a good nutritional source. This study opens a new way to make low-cost medium that can be used in cultivation of <em>Leishmania</em> parasites.</div> Keywords: Leishmania major, lyophilized Rabbit anti-Sheep Cell Haemolysin, fetal calf serum. Culture medium, Haemolysin, Leishmania major, Promastigote 124 130 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-92-1&slc_lang=en&sid=1 Vahid Nasiri وحید نصیری v.nasiri@rvsri.ac.ir 0000-0002-4959-890X Yes Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. Farnoosh Jameie فرنوش جامعی v.nasiri@rvsri.ac.ir 0000-0003-3033-993X No Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. Habibollah Paykari حبیب اله پیکاری v.nasiri@rvsri.ac.ir 0000-0003-4767-1904 No Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. Protozoology Laboratory, Parasitology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran.