en خون شناسی و بانک خون Hematology & Blood Banking پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims:</strong> Myeloid cell leukemia-1 (Mcl-1) plays a pivotal role in the survival of hematologic and solid tumors, and is known as a substantial oncogene. Studies have demonstrated the altered expression of Mcl-1<br> has been linked to malignancy development and poor prognosis. In this research, we have studied the expression of Mcl-1 mRNA in myelodysplastic syndrome (MDS) patients and determined association with clinico-pathological factors, MDS subgroups as well as international prognostic scoring system.<br> <strong>Materials and Methods:</strong> The relative level of Mcl-1 was determined by real time quantitative real-time polymerase chain reaction and gene expression normalized to Glyceraldehyde-3-phosphate dehydrogenase.<br> <strong>Results:</strong> Results indicated amplification of mRNA encoding Mcl-1 in 100% of the cases. The higher level of Mcl-1 existed in MDS patients compared with the healthy controls but there was no statistically difference of Mcl-1 expression between these groups. Fold change in gene expression was higher in advanced stage MDS, high risk MDS, cases with >5% blast and LDH >400 to their corresponding groups. In addition, the correlation between gene expression and cytogenetic prognostic subgroups was statistically significant (p=0.043).<br> <strong>Conclusions:</strong> In the present study, we showed that Mcl-1 is expressed in MDS independent of the World Health Organization subgroup and international prognostic scoring system. Therefore, Mcl-1 may be up-regulated already in early stages of leukemogenesis.</div> Mcl-1, Myelodysplastic syndromes, Real time PCR 43 50 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-300-1&slc_lang=en&sid=1 Farhad Zaker فرهاد ذاکر farhadz20@yahoo.co.uk No Department of Hematology, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Cellular and Molecular Research Center, Dept. of Hematology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Mohamad Jafar Sharifi محمد جعفر شریفی mjsharifi63@yahoo.com 0000-0003-0719-8294 No Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran. Department of immunology, isfahan University of Medical Sciences, Shiraz, Iran Nahid Nasiri ناهید نصیری Nahid.nasiri64@yahoo.com 0000-0002-5995-8745 Yes Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Department of hematology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Marjan Yaghmaie مرجان یغمایی marjan_yaghmaie@yahoo.com No Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran. Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Science, Tehran, Iran Soodeh Namjoo سوده نامجو nsoodeh@yahoo.com No Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran