en باکتری شناسی Bactriology پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims:</strong> Uropathogenic <em>Escherichia coli</em> (UPEC) are considered major reservoir for genes encoding antimicrobial resistance. The mechanism of resistance and persistence of UPEC has been attributed to the production of biofilm and Extended Beta Lactamase (ESBL). This hospital-based prospective study determined how biofilm and ESBL production facilitate antibacterial resistance amongst UPEC isolated from catheter urine of patients attending the University of Ilorin Teaching Hospital, Nigeria.<br> <strong>Materials and Methods:</strong> Urine samples from 113 catheterized inpatients and outpatients were analysed. Female subjects accounted for 47 (41.6%) of the study population. Standard microbiological methods and Analytical Profile Index (API) 20E were used for the isolation and identification of UPEC. Tissue culture plate technique was used to demonstrate biofilm production potentials and double-disc synergy test was used to determine ESBL production.<br> <strong>Results:</strong> Catheter associated urinary tract infection in this study was 70.8% of samples analysed. Of this, <em>Escherichia coli</em>, 44 (55.0%) was the most predominant. UPEC, biofilm and ESBL production amounted to 38.9%, 81.8% and 27.2%, respectively. ESBL production was significantly associated with degree of biofilm formation (p<0.005). Both strong and moderate biofilm producers showed the same level of resistance to ceftazidime (31.6%). Moderate biofilm producers were 46.7% resistant to cefriaxone. Resistance to Amoxillin-clauvanate significantly occurred in all grades of biofilm producers (p>0.05). Imipenem, however, was the most sensitive with no resistance by the UPEC.</div> <div style="text-align: justify;"><strong>Conclusions:</strong> ESBL and biofilm production were associated with antibacterial resistance. The incidence of ESBL production amongst biofilm forming UPEC is of great public health concern.</div> Beta-lactamases, Biofilm, Drug resistance, Uropathogenic Escherichia coli 241 250 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-348-1&slc_lang=en&sid=1 Dele Ohinoyi Amadu Dele Ohinoyi Amadu deleamama@yahoo.com 0000-0003-2332-9856 No Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. Department of Medical Microbiology and Parasitology University of Ilorin Charles Nwabuisi Charles Nwabuisi Charleh@yahoo.com No Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. Yahaya Usman Yahaya Usman Yahyau@yahoo.com 0000-0001-9568-1651 No Department of Medical Laboratory Science, Ahmadu Bello University Zaria, Nigeria. Department of Medical Laboratory Science Ahmadu Bello University Zaria Nigeria Jelili Mustapha Jelili Mustapha Jelili@yahoo.com No Department of Environmental Health, University of Salford, Manchester, UK. Department of Environmental Health, University of Salford, Manchester, UK. Idris Nasir Abdullahi Idris Nasir Abdullahi eedris888@yahoo.com 0000-0002-5511-127 Yes Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. Ademola Popoola Ademola Popoola poppola@yahoo.com 0000-0002-1850-2223 No Department of Surgery, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. Department of Surgery, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria.