en Molecular identification of helicobacter pylori and genes iceA frequency from dental plaques isolated from people using method PCR باکتری شناسی Bactriology پژوهشي Research Abstract:<br> Helicobacter Pylori is a Gram-negative, microaerophilic, spiral-shaped flagellated, bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. Helicobacter pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. The aim of this study was to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd and the percentage of iceA gene examined by PCR technique.<br> &nbsp;<br> Methods:<br> A total of 100 samples of dental plaque were taken and transferred to a tube that had already been physiologically placed. After DNA extraction, primer design was performed and then the PCR was performed for the whole sample.<br> <br> Results:<br> Of 100 samples examined in this study, 2 samples of Helicobacter pylori were positive (2%) and the frequency of the iceA gene of 2 samples was positive (100%).<br> &nbsp;<br> Conclusion:<br> In the Bojnord area, the frequency of iceA gene in people is high and the frequency of HP in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of Helicobacter pylori infection in the region. To ensure this, more molecular studies are suggested in other populations. <div style="text-align: justify;"><strong>Background and Aims:</strong> <em>Helicobacter pylori</em> (<em>H.&nbsp;pylori</em>) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. <em>H. pylori</em> has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd city, and the polymerase chain reaction technique examined the percentage of iceA gene.<br> <strong>Materials and Methods:</strong> A total of 100 samples of dental plaque were taken and transferred to a tube that has been physiologically placed. After DNA extraction, primer design was performed, and then the polymerase chain reaction was performed for the whole sample.<br> <strong>Results: </strong>Of 100 samples examined in this study, two samples of <em>H. pylori</em> were positive (2%), and the frequency of the iceA gene of two samples was positive (100%).<br> <strong>Conclusion:</strong> In the Bojnord city, the frequency of iceA gene in people is high, and the frequency of <em>H.&nbsp;pylori</em> in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of <em>H. pylori</em> infection in the region. To confirm the results, more molecular studies are required in other populations.</div> Helicobacter pylori, Dental plaque, IceA gene, PCR technique Helicobacter pylori, Dental plaque, IceA gene, PCR technique 191 196 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-360-1&slc_lang=en&sid=1 Zahra Salari Zahra Salari ghazale.st2015@gmail.com 0000-0002-1721-3659 Yes Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran Atefeh Ranjkesh Atefeh Ranjkesh Atefeh@yahoo.com 0000-2136-2598-8963 No Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran Emad Behboudi Emad Behboudi emadbehboudi69@gmail.com No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran 2Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran