en ژنتیک/ بیوتکنولوژی Genetics/ Biotechnology پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims:</strong> Tissue engineering is a relatively novel field that has been intensely developing during recent years and has shown to be excessively promising when used for cartilage regeneration. Scaffolds represent important components for tissue engineering.<br> <strong>Materials and Methods:</strong> The Poly Lactic-Co-Glycolic Acid (PLGA) impregnated with fibrin and hyaluronic acid (HA) produce hybrid scaffolds. human adipose-derived stem cells (hADSCs) were seeded in scaffolds and cultured in chondrogenic media. The viability of cells in different groups was assessed by MTT. The expression of chondrogenic related genes [<em>Sox9</em>, <em>type II collagen </em>(<em>Col II</em>), <em>Aggrecan</em>(<em>AGG</em>)] and <em>type X collagen</em> (<em>Col X</em>) was quantified by real-time polymerase chain reaction.<br> <strong>Results:</strong> The results of the real-time PCR showed <em>SOX9</em>, <em>AGG</em> and <em>Col X</em> gene expression in the control groups being significantly lower than the other groups (p&le;0.05). It also demonstrated <em>Col II</em> gene expression in the control group being significantly lower than the PLGA/Fibrin and PLGA/Fibrin/HA groups (p&le;0.05). The <em>Col X</em> gene expression of cells in PLGA/HA and PLGA/Fibrin/HA groups significantly decreased in comparison with the PLGA/Fibrin group (p&le;0.05).<br> <strong>Conclusions:</strong> These conclusions indicate that administration of PLGA/ Fibrin and PLGA/HA scaffolds, particularly PLGA/Fibrin/ HA, motivates chondrogenesis in hADSCs. This can be diminished by decreasing hypertrophic markers and increasing characteristic markers of hyaline cartilage.</div> Copolymer, Hyaluronic acid , Mesenchymal stem cells, Polylactic acid-polyglycolic acid, Tissue scaffolds 128 137 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-379-1&slc_lang=en&sid=1 Zeinolabedin Sharifian زین العابدین شریفیان m.pourentezari@gmail.com No Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Batool Hashemibeni بتول هاشمی بنی m.pourentezari@gmail.com 0000-0001-9656-2355 No Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran. Majid Pourentezari مجید پورانتظاری m.pourentezari@gmail.com 0000-0001-7549-4672 Yes Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ali Valiani علی ولیانی m.pourentezari@gmail.com 0000-0003-3093-5547 No Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran. Mohammad Mardani محمد مردانی m.pourentezari@gmail.com 000-0002-4328-9663 No Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran. Mohammad Zamani Rarani محمد زمانی رارانی m.pourentezari@gmail.com 0000-0003-2841-7927 No Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Hamidreza Pourentezari حمیدرضا پورانتظاری m.pourentezari@gmail.com No Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.