International Journal of Medical Laboratory
مجله بین المللی علوم آزمایشگاهی
IJML
Medical Sciences
http://ijml.ssu.ac.ir
1
admin
2423-3706
2423-3714
en
jalali
1399
8
1
gregorian
2020
11
1
7
4
online
1
fulltext
en
Which Culture System Is better for Chondrogenesis of Adipose-Derived Stem Cells ; Pellet or Micromass?
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><strong>Background and Aims:</strong> The current study was conducted to compare the expression levels of <a name="OLE_LINK1">collagen type Π</a> and X during chondrogenesis of human adipose-derived mesenchymal stem cells (hADMSCs) pellet and micromass cultures. <br>
<strong>Materials and Methods</strong>: Extracted hADMSCs were cultured until three passages and then transferred to pellet and micromass cultures in the experimental groups of day 7 and day14. For <a name="OLE_LINK7"></a><a name="OLE_LINK8">pellet and micromass cultures, aliquots of 5×</a>10<sup>5</sup> cells/ml were centrifuged and respectively cultured in the conical tubes and droplets (12.5 µl) of the 24-well plates containing chondrogenic medium. Realtime-polymerase chain reaction technique was performed for gene expression levels.<br>
<strong>Results:</strong> Increased expression of collagen type Π was shown in micromass day14 compared to micromass day 7, pellet day 14 (p<0.01) and pellet day 7 (p<0.001). Also, an increased expression of collagen type Π was seen in micromass day 7 and pellet day 14 compared to pellet day 7 (p< 0.05). Expression of collagen type X increased in pellet day 14 compared to micromass on days 7 and 14 (p<0.001, p<0.01) and pellet day14 compared to pellet day7 (p< 0.05). An increased expression of collagen type X was shown in pellet day 7 compared to micromass on days 7 and 14 (p<0.05).<br>
<strong>Conclusions</strong>: According to the results, higher expression of collagen type Π and lower expression of collagen type X in micromass cultures that are prepared by cell suspension play a better role during cellular condensation that leads to the formation of large nodules exhibiting cartilage-like morphology, suggests a higher efficiency for micromass cultures.</div>
Chondrogenesis, Collagen, Stem cell
280
288
http://ijml.ssu.ac.ir/browse.php?a_code=A-10-387-1&slc_lang=en&sid=1
Mahtab
Teimouri
مهتاب
تیموری
mahtab.teymuri@yahoo.com