en Effects of topical application of Royal Jelly on second-degree burn wound healing in male rats بیوشیمی Biochemistry پژوهشي Research <div dir="ltr"><strong>Introduction</strong>: burn injury remains as a major medical problem throughout the world. This injury is accompanied with inflammatory and wound healing responses. Since Royal jelly (RJ) has anti-inflammatory and wound healing activity therefore, the aim of this study was to evaluate the repairing effects of RJ on skin burn- damage.</div> <div style="text-align: left;"><strong>Methods</strong>: In an experimental study, 40 male wistar rats (8 weeks old) were engaged. The animals were divided into five equal groups. Group 1 was considered healthy control. Group 2 (positive control) was treated topically with Silver Sulfadiazine Cream, group3 received Eucerin as negative control, and group 4, 5 treated with RJ (10 and 30%).<br> Sampling was performed after observing the second-degree burns on the first, seventh and fourteenth days.&nbsp; Then after 28 days, rats were sacrificed and their skin tissues were used for morphological and morphometric assessments.<br> <strong>Results:</strong> The results of this study showed that the amount and arrangement of collagen type 1 protein was higher in the RJ treatment groups versus control group.<br> Reconstruction and thickening of the epithelium in RJ treated groups confirmed therapeutic effects <em>of RJ</em><em>.</em> In addition, RJ increased angiogenesis compared to the control group. The <em>woundchr(&#39;39&#39;)s surface area</em> was reduced in the RJ treatment groups compared to the control group. In addition, Fibroblast cell proliferation was increased in the <em>groups receiving</em> RJ versus control.</div> <div style="text-align: left;"><strong>Conclusion</strong>: It could be concluded that, RJ induce wound healing effects and might be considered as potential treatment option to improve the burn wound healing.</div> <div style="text-align: justify;"><strong>Background and Aims:</strong> Burn injury remain as a major medical problem throughout the world. This injury is accompanied with inflammatory and wound healing responses. Since royal jelly (RJ) has anti-inflammatory and wound healing activity therefore, the aim of this study was to evaluate the repairing effects of RJ on skin burn- damage.<br> <strong>Materials and Methods</strong>: In an experimental study, 40 male Wistar rats (8 weeks old) were engaged. The animals were divided into five equal groups. Group 1 was considered healthy control. Group 2 (positive control) was treated topically with Silver Sulfadiazine Cream, group 3 received Eucerin as negative control, and group 4, 5 treated with RJ (10 and 30%). Sampling was performed after observing the second-degree burns on the first, seventh and fourteenth days. Then after 28 days, rats were sacrificed and their skin tissues were used for morphological and morphometric assessments.<br> <strong>Results:</strong> The results of this study showed that the amount and arrangement of collagen type 1 protein was higher in the RJ treatment groups versus control group. Reconstruction and thickening of the epithelium in RJ treated groups confirmed therapeutic effects <em>of RJ</em><em>.</em> In addition, RJ increased angiogenesis compared to the control group. The <em>wound&#39;s surface area</em> was reduced in the RJ treatment groups compared to the control group. In addition, fibroblast cell proliferation was increased in the <em>groups receiving</em> RJ versus control.<br> <strong>Conclusions</strong>: It could be concluded that, RJ induce wound healing effects and might be considered as potential treatment option to improve the burn wound healing.</div> Royal jelly, Inflammation, Wound, Burn injury. Burns, Inflammation, Royal jelly, Wound healing 289 299 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-388-1&slc_lang=en&sid=1 Zeinab Mahdian زینب مهدیان mahdian.zeinab 0210@gmail.com 0000-0002-6050-6076 No Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Fereshteh Amiri فرشته امیری fereshtehamiri86@yahoo.com 0000-0001-6036-5122 No Department of Biology, Science and Reserch branch, Islamic Azad University, Tehran, Iran Zohreh Mohammadi زهره محمدی YRC1365@GMAIL.COM 0000-0003-0967-3781 No Department of Microbiology, Islamic Azad University, Kerman branch, Kerman, Iran. Parviz Farzadinia پرویز فرزادی نیا bazzy_par@yahoo.com 0000-0003-0315-9933 No Department of Biology and Anatomical Sciences, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Fahimeh Safizadeh فهیمه صفی زاده FahimeSafizade67@gmail.com No Iranian Social Security Organization, Kerman, Iran. Zahra Zare زهرا زارع z.zare@cfu.ac.ir No Department of Biology, Farhangian university, Tehran, Iran. Rahimeh Rahimi رحیمه رحیمی Rahimehrahimi2023@gmail.com 0000-0002-1084-8801 No Department of Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Zahra Dehghani زهرا دهقانی Zahradehghani111070@yahoo.com 0000-0002-2807-6969 No Department of Pathology, Bushehr University of Medical Science, Bushehr, Iran. Fariba Mohammadi Tahroodi فریبا محمدی تهرودی faribamt93@yahoo.com 0000-0002-2798-6637 Yes Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran