International Journal of Medical Laboratory

en Effect of Peganum Harmala Seeds Extract on Nitric Oxide in U937 Monocytes and Macrophages ایمنی شناسی Immunology پژوهشي Research <div dir="ltr">Background: Nitric oxide (NO) has an important role in inflammation and has been related with pathogenesis and progress of numerous inflammatory- based diseases including some cancers. Peganum harmala is a medicinal plant has been used for treatment of numerous diseases such as several infections. Also anti-inflammatory effects of peganum harmala extracts and its derivatives (harmaline and harmine) by suppressing myeloperoxidase, NO and other mediators have been demonstrated in vivo. In this study, effect of P. harmala seeds aquaeus extract on NO production in U937 monocytic cells and peritoneal macrophages has been evaluated in vitro. Methods: U937 and mice peritoneal macrophages were cultured in RPMI with 10% FBS. Then the cells at logarithmic growth phase were incubated with different concentrations of aqueous extract of P. harmala seeds (0.1 – 1 mg/ml) for 24 hours. Then NO production was assessed by the Griess method in culture medium. Data was analyzed by analysis of variance (ANOVA). Results: P. harmala seeds aqueous extract did not show any significant effect on LPS-induced NO production in U937 cells and peritoneal macrophages after 24 hours incubation time compared with untreated control cells. Conclusion: According to results of this study, aqueous extract of P. harmala seeds has no effect on NO production in U937 monocytes cells and peritoneal macrophages. These results suggest that anti-inflammatory effects of P. harmala may be mediated through NO -independent mechanism(s). However further studies to define the P. harmala aqueous extract impact on NO expression in other related normal and cancerous cells are warranted.</div> <div style="text-align: left;"></div> <div style="text-align: justify;">Background and Aims: Nitric oxide (NO) has an essential role in inflammation and has been related to pathogenesis and the progress of numerous inflammatory-based diseases, including some cancers. Peganum harmala (P. harmala) is a medicinal plant used for the treatment of numerous diseases such as several infections. Also, anti-inflammatory effects of P. harmala extracts and its derivatives (harmaline and harmine) by suppressing myeloperoxidase, NO, and other mediators have been demonstrated in vivo. In this study, the effect of P. harmala seeds aqueous extract on NO production in U937 monocytic cells and peritoneal macrophages has been evaluated in vitro. Materials and Methods: U937 and mice peritoneal macrophages were cultured in <a href="https://en.wikipedia.org/wiki/Roswell_Park_Memorial_Institute" title="">Roswell Park Memorial institute</a>-1640 with 10% fetal calf serum. Then, the cells at the logarithmic growth phase were incubated with different concentrations of aqueous extract of P. harmala seeds (0.1-1 mg/ml) for 24 hours. Next, NO production was assessed by the Griess method in the culture medium. Results: P. harmala seeds aqueous extract did not significantly affect lipopolysaccharide-induced NO production in U937 cells and peritoneal macrophages after 24 hours incubation time compared with untreated control cells. Conclusion: These results suggest that the anti-inflammatory effects of P. harmala may be mediated through NO-independent mechanism(s). However, further studies are warranted to define the P. harmala aqueous extract impact on NO expression in other related normal and cancerous cells.</div> Peganum harmala, nitric oxide, U937, macrophages Macrophages, Nitric oxide, Peganum harmala, U937 312 320 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-391-1&slc_lang=en&sid=1 Nima Rahmati نیما رحمتی dr.nimarahmati@gmail.com 0000-0002-5049-9650 No Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran گروه ایمنی شناسی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران Fatemeh Hajighasemi فاطمه حاجی قاسمی fatimahajighasemi@gmail.com 0000-0003-0145-4621 Yes Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran گروه ایمنی شناسی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران