en بیوشیمی Biochemistry پژوهشي Research <div style="text-align: justify;"><strong>Background and Aims: </strong>Mesenchymal stem cells (MSCs) are a preferred cell source for the generation of hepatocyte-like cells in regenerative medicine. They can be isolated from different sources, including adipose tissues. The Raman spectroscopy approach was evaluated for quick and efficient identification of MSCs differentiation status and a broader perspective on cell differentiation.<br> <strong>Materials and Methods</strong>: The human adipose-derived mesenchymal stem cells (hASCs) were differentiated toward hepatocyte-like cells using a well-established method. The cells were cultured on fluorescence-free quartz discs, and the efficiency of differentiation was examined using molecular and biochemical methods. The Raman spectra were recorded at days 1, 7, 14, and 21 of differentiation, and HepG2 was used as a positive control.<br> <strong>Results</strong>: The changes in Raman spectra were detected during the sequential stages of differentiation, and the pattern of peaks on the last day of differentiation was remarkably similar to the positive control (HepG2).<br> <strong>Conclusion</strong>: Raman spectroscopy showed considerable potential to characterize hepatic differentiation.</div> Adipose tissue, Hepatocyte-like cells, Mesenchymal stem cells, Raman spectroscopy 122 130 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-402-1&slc_lang=en&sid=1 Sareh Arjmand ساره ارجمند s_arjmand@sbu.ac.ir No Protein Research Center, Shahid Beheshti University, G. C., Tehran, Iran دانشگاه شهید بهشتی، مرکز تحقیقات پروتئین Aram Barzegar آرام برزگر aram.barzegar@yahoo.com No Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran دانشگاه تربیت مدرس، دپارتمان فیزیک Alemeh Mohammadpour عالمه محمدپور alememohamadpoor@gamil.com No Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran دانشگاه تربیت مدرس، دپارتمان بیوشیمی بالینی Hanieh Rezaei هانیه رضایی rezaei.hanieh@yahoo.com No Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran دانشگاه تربیت مدرس، دپارتمان فیزیک Nahid Davoodian ناهید داوودیان nahid_davoodian@yahoo.com No Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran دانشگاه علوم پزشکی هرمزگان، مرکز تحقیقات غدد و متابولیسم Hori Ghaneialvar حوری قانعی الوار ghaneihuri@yahoo.com No Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran دانشگاه علوم پزشکی ایلام، دپارتمان بیوشیمی بالینی Rasoul Malekfar رسول ملک فر , malekfar@modares.ac.ir No Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran دانشگاه تربیت مدرس، دپارتمان فیزیک Abbas Sahebghadam Lotfi عباس صاحبقدم لطفی lotfi_ab@modares.ac.ir Yes Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran دانشگاه تربیت مدرس، دپارتمان بیوشیمی بالینی