en ویروس شناسی Virology پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Background and Aims: </span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">Currently, there are antiviral chemicals used to treat viral infections accompanied by limitations such as high levels of toxicity and adverse effects in humans, the emergence of drug-resistant viral strains, low numbers, and limited diversity. Therefore, it is necessary to evaluate new photochemical to obtain new therapeutic methods. The present study was conducted to evaluate the antiviral activity of <i>Arnebia Euchroma </i>(<i>A. Euchroma</i>) extract, amniotic membrane, a mixture of <i>A. euchroma</i> extract and amniotic membrane and its carrier (comprised of Sesam and ostrich oil) against three different viruses, including Herpes simplex virus type 1 (HSV-1), influenza A, and rotavirus <i>in-vitro</i>.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods</span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">: A methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the cytotoxic effect of the above compounds on Vero, MDCK, and MA-104 cell lines. After the determination of non-toxic concentrations, Tissue culture infection dose 50 (TCID50) test was performed to determine antiviral activity.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results</span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">: Among all the above-mentioned compounds, the combination of <i>A. euchroma</i> extract and amniotic membrane at the highest non-toxic concentration for the cells had the highest antiviral activity against all three viruses, leading to 1 log10 TCID50 reduction in influenza A and HSV-1 titers and 0.6 log10 TCID50 reductions in rotavirus titer when compared to the virus control.</span></span></span></span></span><br> <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The combination of <i>A. euchroma</i> and amniotic membrane can be considered a new antiviral agent to treat viral infections.</span></span></div> Amniotic membrane, Arnebia euchroma, Herpes simplex virus, Influenza Rotavirus 237 247 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-406-2&slc_lang=en&sid=1 Mohammed Mehdi Saghafi سید مهدی ثقفی saghafi.mm@iums.ac.ir 0000-0003-4469-1408 No Pharmaceutical Care Department, Firozabadi Hospital, Iran University of Medical Sciences, Tehran, Iran Ahmad Tavakoli احمد توکلی Ahmad.tavakoli66@gmail.com 0000-0003-1857-0610 No Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Shahnaz Ahmadi شهناز احمدی Ahmadi.sh@iums.ac.ir 0000-0002-2136-4474 No Department of Obstetrics and Gynecology, Iran University of Medical Sciences, Tehran, Iran Seyed Hamidreza Monavari سید حمیدرضا منوری hrmonavari@yahoo.com 0000-0003-4475-443x Yes Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran