International Journal of Medical Laboratory

en Cutaneous Leishmaniasis in Suspected Patients Referred to the Healthy Centrals of Abarkouh, Ardakan, Bafgh and Khatam, Yazd Province, Iran Cutaneous Leishmaniasis in Suspected Patients Referred to the Healthy Centrals of Abarkouh, Ardakan, Bafgh and Khatam, Yazd Province, Iran انگل شناسی Parasitology پژوهشي Research <div dir="ltr" style="text-align: justify;">Background and Aims: Cutaneous Leishmaniasis is a significant health problem in many parts of Iran. Management of the disease and its treatment is a global dilemma. In this study, the status and the proportions of Cutaneous Leishmaniasis induced by Leishmania major and tropica among suspected patients referred to the Health Centers of Abarkouh, Ardakan, Bafgh, and Khatam cities, Yazd Province, Iran were investigated. Materials and Methods: The lesion was diagnosed using direct smear microscopy and conventional polymerase chain reaction. Results: A total of 90 samples were prepared of which 64 (71.1%)  were male and 26 (27.9%) were female. Also, 30 (33.3%) samples came  from Ardakan, 29 (32.2%) samples  from Bafgh, 21 (23.3%) samples from Abarkouh, and 10 (11.1%) samples  from Khatam city. Ninety samples with 112 lesions were recruited and parasitologically examined. The results showed that, in macroscopic examination, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major = 52 (M=35, 67.3%, F=17, 32.7%), lesion(s) induced by Leishmania tropica =4 (M=2, 50.0%, F=2, 50.0%), and lesion(s) induced by false positive =33 (36.4%). Basides, in microscopic method, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major =44 (M=29, 65.9%, F=15, 34.09%), lesion(s) induced by Leishmania tropica =9 (M=8, 88.8%, F=1, 11.2%), and lesion(s) induced by false positive =37 (M=28, 75.7%, F=9, 24.3%).  Conclusion: Although travel history to an endemic area is important for diagnosis, parasitological confirmation is necessary to initiate treatment.</div> <div style="text-align: justify;">Background and Aims: Cutaneous Leishmaniasis is a significant health problem in many parts of Iran. Management of the disease and its treatment is a global dilemma. In this study, the status and the proportions of Cutaneous Leishmaniasis induced by Leishmania major and tropica among suspected patients referred to the Health Centers of Abarkouh, Ardakan, Bafgh, and Khatam cities, Yazd Province, Iran were investigated. Materials and Methods: The lesion was diagnosed using direct smear microscopy and conventional polymerase chain reaction. Results: A total of 90 samples were prepared of which 64 (71.1%)  were male and 26 (27.9%) were female. Also, 30 (33.3%) samples came  from Ardakan, 29 (32.2%) samples  from Bafgh, 21 (23.3%) samples from Abarkouh, and 10 (11.1%) samples  from Khatam city. Ninety samples with 112 lesions were recruited and parasitologically examined. The results showed that, in macroscopic examination, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major = 52 (M=35, 67.3%, F=17, 32.7%), lesion(s) induced by Leishmania tropica =4 (M=2, 50.0%, F=2, 50.0%), and lesion(s) induced by false positive =33 (36.4%). Basides, in microscopic method, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major =44 (M=29, 65.9%, F=15, 34.09%), lesion(s) induced by Leishmania tropica =9 (M=8, 88.8%, F=1, 11.2%), and lesion(s) induced by false positive =37 (M=28, 75.7%, F=9, 24.3%).  Conclusion: Although travel history to an endemic area is important for diagnosis, parasitological confirmation is necessary to initiate treatment.</div> سالک, ابرکوه, اردکان, بافق, خاتم, PCR Cutaneous Leishmaniasis, Leishmania major, Leishmania tropica, PCR, Yazd 215 222 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-31-4&slc_lang=en&sid=1 Somayeh Ahmadi سمیه احمدی saria.ahmadi@yahoo.com 0000-0002-0920-2456 No Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran 1.گروه انگل شناسی و قارچ شناسی دانشکده پزشکی دانشگاه علوم پزشکی شهید صدوقی Gilda Eslami گیلدا اسلامی eslami_g2000@yahoo.com 0000-0002-4521-4059 No Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran 1.گروه انگل شناسی و قارچ شناسی دانشکده پزشکی دانشگاه علوم پزشکی شهید صدوقی Mahmoud Vakili محمود وکیلی vakilim52@yahoo.com 0000-0002-0113-7100 No Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran دانشگاه علوم پزشکی شهید صدوقی- دانشکده پزشکی- گروه عمومی Kazem Barzegar کاظم برزگر farzadbavanati1350@gmail.com 0000-0001-5766-2214 No Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran دانشگاه علوم پزشکی شهید صدوقی- دانشکده پزشکی- گروه عمومی Ali Fattahi bafghi علی فتاحی بافقی a.fattahi@ssu.ac.ir 0000-0002-8469-2187 Yes Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Infectious Diseases Research Center, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran .گروه انگل شناسی و قارچ شناسی دانشکده پزشکی دانشگاه علوم پزشکی شهید صدوقی