en باکتری شناسی Bactriology پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Background and Aims: </span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">Hospital infections and their antibiotic resistance have become a global concern recently. One of the most prominent factors in hospital infections is <i>Pseudomonas aeruginosa (P.&nbsp;aeruginosa),</i> which can become resistant to many antibiotics due to its ability to form biofilms. Recently, scientists have tried to replace antibiotic therapy with alternative therapies such as probiotics which can reduce or eliminate the <span style="background:white">pathogenic bacteria&#39;s ability to form biofilms.</span> Therefore, the present study revealed that some genes, such as <i>algD </i><br> and<i> PpyR</i>, were involved in biofilm formation in <i>P.&nbsp;aeruginosa</i>. Furthermore, the inhibitory effect of the supernatant of <i>lactobacillus agilis</i> on the biofilm formation of <i>P.&nbsp;aeruginosa</i> was evaluated in the current study.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods: </span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">In this study, the effect of the supernatant of probiotic <i>Lactobacillus agilis</i> on the biofilm formation of <i>P.&nbsp;aeruginosa</i> and also the expression of two genes effective in biofilm formation (<i>algD</i> and <i>PpyR) </i>were investigated. Antibiograms were performed to detect the most resistant bacteria since there is a link between biofilm formation and antibiotic resistance. Further, the effects of probiotics on the expression of <i>PpyR</i> and <i>algD </i>genes were discussed.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results: </span></span></b><span style="font-size:10.0pt"><span style="line-height:115%">Results showed that the biofilm </span></span><span style="font-size:10.0pt"><span style="line-height:115%">formation of <i>P.&nbsp;aeruginosa</i></span></span><span style="font-size:10.0pt"><span style="line-height:115%"> was significantly reduced in the presence of </span></span><i><span style="font-size:10.0pt"><span style="line-height:115%">lactobacillus agilis</span></span></i><span style="font-size:10.0pt"><span style="line-height:115%">.</span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"></span></span></span></span></span></span><br> <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></b> <span style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times="">According to the current study, it could be concluded that because of antibiotics resistance and their associated mechanisms, probiotics could be used as a replacement for antibiotics in many treatments</span></span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span></i></div> <span style="font-size:11pt"><span style="line-height:150%"><span sans-serif="" style="font-family:Calibri,"><i><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""></span></span></span></i></span></span></span> algD, Biofilm, Gene expression, PpyR, Pseudomonas aeruginosa 23 34 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-489-1&slc_lang=en&sid=1 Yasaman Issazadeh یاسمن عیسی زاده yasamanissazadeh@gmail.com 0000-0002-6749-9569 No Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Mohadeseh Farnaghizad محدثه فرنقی زاد mohadesehfarnaghizad@gmail.com No Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Sarvenaz Falsafi سروناز فلسفی sarvenaz_falsafi@yahoo.com No Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran Hoora Mazaheri حورا مظاهری hooram22@yahoo.com No Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Shokoofeh Ghazi شکوفه غازی shokoofeh_ghazi@gmail.com No Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Ava Behrouzi آوا بهروزی ava.behrouzi@gmail.com 0000-0001-8677-6851 Yes Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran