International Journal of Medical Laboratory
مجله بین المللی علوم آزمایشگاهی
IJML
Medical Sciences
http://ijml.ssu.ac.ir
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admin
2423-3706
2423-3714
en
jalali
1401
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gregorian
2022
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online
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تنوع ژنتیکی ژن های COX1 میتوکندری و ITS2 ریبوزومی توکسوکارا کانیس در سگ های ولگرد اهل زابل، جنوب شرق ایران
Genetic Variation in Mitochondrial COX1 and Ribosomal ITS2 Genes of Toxocara Canis in Stray Dogs in Zabol, Southeast Iran
انگل شناسی
Parasitology
پژوهشي
Research
<p> </p>
<p style="text-align: justify;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span style="font-size:10.0pt">Background and Aims: </span></b><span style="font-size:10.0pt"><span style="color:#0e101a">Toxocariasis is a zoonotic parasitic disease with worldwide distribution caused by the larval stage of ascaridoid nematodes of dogs (<em>Toxocara canis</em>) and cats (<em>Toxocara cati</em>). The study was accomplished to determine the sequence variation in <i>ITS2</i> and <i>COX1</i> genes within isolates of <em>Toxocara canis</em>.</span></span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span style="font-size:10.0pt"><span style="color:#0e101a">Materials and Methods:</span></span></b><span style="font-size:10.0pt"><span style="color:#0e101a"> Two hundred Stool samples were collected randomly from dogs in public parks and streets from different regions of Zabol. Thirty samples containing eggs were isolated from the feces using Formalin ether 10% and centrifugal flotation. Genomic DNA was extracted, and <i>COX1</i> and <i>ITS2</i> were amplified by PCR-RFLP and sequenced. Sequence data were aligned using the BioEdit software and BLAST program and compared with published sequences in GenBank. The phylogenetic relationship between isolates of <em>T. canis</em> from Zabol city with other regions based on sequences obtained from <i>COX1</i> and <i>ITS2</i> genes and using MEGA7.0 software was investigated.</span></span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span style="font-size:10.0pt"><span style="color:#0e101a">Results: </span></span></b><span style="font-size:10.0pt"><span style="color:#0e101a">For all samples, amplicons of about 388 and 422 base pairs were produced by PCR for <i>ITS2</i> and <i>COX1</i>, respectively. Drawing the phylogenic tree of <i>ITS2</i> and <i>COX1</i> sequences of isolates of</span></span> <em><span style="font-size:10.0pt"><span style="color:#0e101a">T. canis</span></span></em><span style="font-size:10.0pt"><span style="color:#0e101a"> showed that the identified genotypes are not only different from each other but also from other parts of the world.</span></span></span></span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:#0e101a">Conclusions:</span></span></b><span style="font-size:10.0pt"><span style="color:#0e101a"> Our result showed that genetic variation among isolates of <em>T. canis</em> from</span></span><strong> </strong><span style="font-size:10.0pt"><span style="color:#0e101a">Zabol is very low. For a deeper understanding of genetic diversity among populations of <i>Toxocara</i>, it is recommended to analyze more isolates from various geographical areas and variable genetic markers.</span></span><span style="font-size:10.0pt"></span></span></span><br>
</p>
Genetic variation, Mitochondrial DNA, Ribosomal DNA, Toxocara canis
286
294
http://ijml.ssu.ac.ir/browse.php?a_code=A-10-492-1&slc_lang=en&sid=1
Esmaeil
Sarani
اسماعیل
سارانی
esmailsarani7@gmail.com