en باکتری شناسی Bactriology پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Background and Aims:</span></span></b> <span style="font-size:10.0pt"><span style="line-height:115%">Recurrent pregnancy loss (RPL) is one of the important complications of pregnancy. Only 50% of cases have a known cause of RPL. One of the causes of RPL is <i>Waddlia Chondrophila</i> (<i>W. Chondrophila</i>), but no information is available regarding its in Iran. This study aimed to develop a Taqman Real-Time polymerase chain reaction assay to detect <i>recA</i> Gene of <i>W. Chondrophila</i>in biological samples from women with RPL in Yazd city. </span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods:</span></span></b> <span style="font-size:10.0pt"><span style="line-height:115%">Clinical samples of women with a history of RPL, normal childbirth, and standard strain WSU 86&ndash;1044 were provided. Primers and probes of <i>W. Chondrophila </i>were designed. Positive control was provided based on the PUC57 vector. Technical performance was examined using a control plasmid.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results:</span></span></b> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">Analytical sensitivity was 5 ng/&micro;l</span></span></span></span></span></span></span> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">of control plasmid DNA. No cross-amplification was observed when testing other pathogens that can detect in human vaginitis. The Taqman Real-Time PCR</span></span></span></span></span></span></span> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">assay</span></span></span></span></span></span></span> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">exhibited good reproducibility. This</span></span></span></span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:115%">Real-Time PCR </span></span><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">was then applied to 100 vaginal swabs. No samples were revealed to be </span></span></span></span></span></span></span><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">Waddlia</span></span></span></i></span></span></span></span> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-weight:normal">positive.</span></span></span></span></span></span></span></span></span></span><br> <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusions</span></span><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt">: </span></span></span></span></span></b><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="font-weight:normal">This new TaqMan Real</span></span></span></span></span></span><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt">-</span></span></span></span></span><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="font-weight:normal">Time</span></span></span></span></span></span> <span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="font-weight:normal">PCR</span></span></span></span></span></span><b> </b><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="font-weight:normal">assay represents a diagnostic tool that may be used to further study the prevalence of </span></span></span></span></span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">W.<b> </b>Chondrophila<b> </b></span></span></i><span class="fontstyle01" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:bold"><span style="font-style:normal"><span style="font-size:10.0pt"><span style="font-weight:normal">infection in clinical specimens.</span></span></span></span></span></span></div> Recurrent pregnancy loss, Taqman real-time PCR, Waddlia chondrophila 278 285 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-496-1&slc_lang=en&sid=1 saeed harirzadeh سعید حریرزاده harirzadehsaeed@gmail.com 0000-0001-6606-7783 No Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Farshid Kafilzadeh فرشید کفیل زاده f.kafilzadeh@gmail.com 0000-0002-6772-3989 Yes Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Hengameh zandi هنگامه زندی hengameh_zandi@yahoo.com No Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran PhD in Microbiology Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University Mohamad kargar محمد کارگر mkargar@jia.ac.ir No Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran