International Journal of Medical Laboratory

en اثرات بهبود دهنده یک ترکیب ترکیبات گیاهی در برابر دیس بیوز ناشی از رژیم غذایی پرچرب AGE در موش Effects of the Phytocompound Combination against Dysbiosis Induced by AGE-Rich High-fat Diet in Mice باکتری شناسی Bactriology پژوهشي Research <div style="text-align: justify;">Background and Aims: The composition of the Microbiota can be influenced by various lifestyle and environmental factors, including diet. We designed a study to investigate the improving effects of plant extract, the combination of turmeric, ginger, boswellia, and cat’s claw on the abundance of key members of the gut microbiota, Bacteroidetes, Akkermansia muciniphila (A. muciniphila), Faecalibacterium prausnitzii (F. prausnitzii), Firmicutes and Bifidobacterium in mice treated with advanced glycation end products (AGE) rich high-fat diet (HFD). Materials and Methods: Eighteen 2-month age male C57BL/6 mice were adopted to a regular diet for 1 week and then fed with an HFD or regular diet. After 8 weeks of diet, animals received plant extract concurrently with HFD for 8 weeks. Stools were taken, DNA of stool samples was extracted, and qPCR of 16s rDNA universal primers was performed. Then the effect of plant extract on dysbiosis induced by AGE-rich HFD was accessed. Results: <a name="_Hlk129013683">Our results revealed </a>the frequencies of <a name="_Hlk129100908">Bacteroidetes </a>(p=0.001), A. muciniphila (p= 0.0005), F. prausnitzii (p<0.0001), and Bifidobacterium (<a name="_Hlk129339118">p=</a> 0.0008) were reduced, whereas the frequency of <a name="_Hlk129081622">Firmicute</a> was increased in the AGE-rich HFD group. A significant increase in the F/B ratio was observed in the HFD group than in the regular diet group (p= 0.0003). plant extract reduced the F/B ratio and improved gut microbiota homeostasis.  Conclusion: Plant extract restored gut microbiota patterns in HFD-treated mice. It seems more studies are required to prove the application of plant extracts for modulating the gut microbiota <a name="_Hlk130082090">as a promising new biomarker with potential for therapeutic applications</a>.</div> Advanced glycation end products, Bacteroidetes, Dysbiosis, Gastrointestinal microbiome, Plant extracts 157 165 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-502-1&slc_lang=en&sid=1 Fahimeh Ghanbari فهیمه قنبری gh.fahimeh63@yahoo.com 0000-0002-0360-9001 No Department of Medical Genetics, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Genetics, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Navid Abedpoor نوید عابدپور abedpoor.navid@yahoo.com 0000-0003-1676-6951 No Department of Physiology, Medicinal Plants Research Center, Khorasgan Branch, Islamic Azad University, Isfahan, Iran Department of Physiology, Medicinal Plants Research Center, Khorasgan Branch, Islamic Azad University, Isfahan, Iran Maryam Peymani مریم پیمانی peymani62_m@yahoo.com 0000-0001-5075-5661 No Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran seyedeh-leili Asadi-Yousefabad سیده لیلی اسدی یوسف آباد asadi_427@yahoo.com 0000-0003-2643-7748 No Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Marzieh Lotfi مرضیه لطفی marzeih.lotfi@gmail.com 0000-0001-7323-6717 No Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohammad Hasan Sheikhha محمدحسن شیخها No Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mahta Mazaheri مهتا مظاهری mazaheri54@yahoo.com 0000-0003-4164-3400 Yes Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran