International Journal of Medical Laboratory
مجله بین المللی علوم آزمایشگاهی
IJML
Medical Sciences
http://ijml.ssu.ac.ir
1
admin
2423-3706
2423-3714
en
jalali
1402
8
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gregorian
2023
11
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10
4
online
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fulltext
en
The Relationship of Toxoplasma Gondii Infection with Men Infertility: A Serological and Molecular Study
انگل شناسی
Parasitology
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Background and Aims:</span></span></b><i><span style="font-size:10.0pt"><span style="line-height:115%"> Toxoplasma gondii</span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"> (<i>T. gondii</i>) is one of the most dangerous parasitic infections in immunocompromised patients. It may pass through the bloodstream to various organs, such as the reproductive organs of intermediate hosts, which may lead to male infertility. Therefore, this study aimed to determine the relationship between <i>T. gondii </i>infection and infertility in infertile men referred to the infertility center of Chaharmahal and Bakhtiari province.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods:</span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"> In this case-control study,129 men, including 67 infertile and 62 fertile men, participated, and specific anti-Toxoplasma<i> </i>antibodies of IgG and IgM were detected in the serum of all cases using enzyme-linked immunosorbent assay methods. Also, the presence of <i>T.gondii </i>DNA in the seminal fluid samples of these people was detected using the polymerase chain reaction method. Sperm analyses, including sperm count, morphology, motility, and viscosity, were also performed on the seminal fluid. </span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results:</span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"> Based on the result of the IgG antibody, a significant difference was found in toxoplasmosis<i> </i>in two fertile and infertile groups. Its frequency was 16.1% in the fertile group and 40.3% in the infertile group. Also, Toxoplasma<i> </i>infection showed a relationship with sperm count and sperm morphology, so its frequency was 72.7% in the low sperm group and 24.6% in the high sperm group. The results of IgM and polymerase chain reaction showed, there was no significant relationship between the fertile and infertile groups with toxoplasmosis.</span></span></span></span></span><br>
<b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The serum prevalence of the anti-Toxoplasma<i> </i>antibodies and molecular detection in infertile men is higher than the control group, and it seems that <i>Toxoplasma </i>may be an effective risk factor for causing male infertility and disruption of sperm parameters.</span></span></div>
Infertility, Men, PCR, Toxoplasma gondii
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http://ijml.ssu.ac.ir/browse.php?a_code=A-10-245-1&slc_lang=en&sid=1
Fahimeh
Eshraghi Samani
فهیمه
اشراقی ثمانی
Feshraghi.s@gmail.com