International Journal of Medical Laboratory
مجله بین المللی علوم آزمایشگاهی
IJML
Medical Sciences
http://ijml.ssu.ac.ir
1
admin
2423-3706
2423-3714
en
jalali
1402
8
1
gregorian
2023
11
1
10
4
online
1
fulltext
en
The Effects of Vitamin C on Histological Changes Induced by Acrylamide on Rat Ovary
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Background and Aims:</span></span></b><i> </i><span style="font-size:10.0pt"><span style="line-height:115%">Acrylamide (ACR) adverse effects</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> have been expressed in various tissues.</span></span> <span style="font-size:10.0pt"><span style="line-height:115%">Vitamin C</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> protects the tissue from oxidative damage. </span></span><span style="font-size:10.0pt"><span style="line-height:115%">The goal of this study was to examine the protective<i> </i>effects of vitamin C on histopathological changes induced by ACR on rat ovaries.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods:</span></span></b> <span style="font-size:10.0pt"><span style="line-height:115%">In this experimental study, 28 adult female Wistar rats were used. The animals (n=7) were divided into 4 groups (1) Control group, (2) ACR (10 mg/kg) group, (3) vitamin C (200 mg/kg), (4) ACR [10 mg/kg) + vitamin C (200 mg/kg) daily for 35 days. After the last oral dose, the ovaries were removed placed in the tissue processor, and molded. </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">To determine the volume of the ovary and the number of follicles, we used the </span></span><span style="font-size:10.0pt"><span style="line-height:115%">Cavalieri principle </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">and the physical dissector, respectively</span></span><span style="font-size:10.0pt"><span style="line-height:115%">. </span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results:</span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">The results showed ACR increased the volume of the ovary (p ≤ 0.05), the volume of the medulla (p ≤ 0.01), and the decrease in the volume of the cortex (p ≤ 0.01) in comparison with the control group. These parameters were improved in the ACR + </span></span><span style="font-size:10.0pt"><span style="line-height:115%">vitamin C</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> group. The total number of primordial, primary, secondary, and graafian follicles showed a significant reduction in the ACR group compared to the other groups. The total number of follicles was increased in the ACR + </span></span><span style="font-size:10.0pt"><span style="line-height:115%">vitamin C</span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> group. </span></span></span></span></span><br>
<b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Based on this study, we concluded that</span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">vitamin C can improve histopathological changes of the ovary in ACR-exposed rats.</span></span></div>
Acrylamide, Ovary, Rat, Stereology, Vitamin C
347
356
http://ijml.ssu.ac.ir/browse.php?a_code=A-10-379-7&slc_lang=en&sid=1
Nadia
Ghaderi
نادیا
قادری
ghdri.r.77@gmail.com