en بیوشیمی Biochemistry پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">Introduction: </span></span></span></span></b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">Methanol (CH₃OH) exposure can be highly toxic to humans, resulting in severe clinical manifestations and even deaths if left untreated. Therefore, it is vital to estimate the amounts of methanol quickly and accurately to avoid its adverse health impacts. The main goal of the current study was to develop a simple, fast, reliable, and cost-effective colorimetric method for determining methanol amounts in some alcoholic and non-alcoholic samples.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">Materials and Methods: </span></span></span></span></b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">A sodium nitroprusside-based colorimetric method was established to detect methanol in hand sanitizers, alcoholic beverages, and herbal distillations. A gas chromatography-mass spectrometry apparatus was applied to evaluate the efficacy of the Kit.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">Results: </span></span></span></span></b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">The naked eye saw the color change in the presence of methanol. <a name="_Hlk151869136">The established method revealed a good sensitivity (0.077%) and accuracy for methanol determination in different samples. Besides, this colorimetric method was precise, with intra- and inter-assay coefficients of variations less than 10%. Notably, the recovery percentages were 96.5% to 108%, indicating the acceptable accuracy of the studied method</a></span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black">.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"><span style="color:black"> The quantitative detection of methanol was finally validated by comparing it with gas chromatography-mass spectrometry results.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:115%"></span></span></span></span></span></span><br> <b><span style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusions: </span></span></span></span></b><span style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">This colorimetric method has great potential for methanol detection due to advantages such as good sensitivity, acceptable accuracy, and fast response time.</span></span></span></span></div> Colorimetry, Methanol, Poisoning 15 27 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-529-1&slc_lang=en&sid=1 Ali Bakhshi علی بخشی ali.bakhshi199425@gmail.com No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی، یزد، ایران Mohamadreza Savaee محمدرضا ساوایی tasnim.niayesh313@gmail.com No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی، یزد، ایران Koorosh Goodarzvand Chegini کوروش گودرزوند-چگینی Kooroshgoodarzvandchegini@gmail.com 0000-0002-6149-9605 No Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی، یزد، ایران Mehdi Hedayati مهدی هدایتی hmehdi1403@gmail.com 0000-0001-5816-775X Yes Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات غدد سلولی و مولکولی، پژوهشکده علوم غدد درون ریز، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران