International Journal of Medical Laboratory

en بررسی مولکولی پنج ویروس تنفسی در بیماران مبتلا به عفونت حاد دستگاه تنفسی Molecular Investigation of Seven Respiratory Viruses in Patients with Acute Respiratory Tract Infections ویروس شناسی Virology پژوهشي Research سابقه و هدف: عفونت دستگاه تنفسی یکی از شایع ترین عواملی است که سلامت انسان را تهدید می کند و یکی از علل عمده مرگ و میر به ویژه در کودکان زیر 5 سال است. ویروس ها مسئول تقریباً 80 درصد عفونت های حاد دستگاه تنفسی (ARI) در سراسر جهان هستند. مطالعه ما با هدف گزارش اپیدمیولوژی مولکولی هفت ویروس تنفسی در RTI انجام شد. این نتیجه می تواند به توسعه استراتژی های بهداشت عمومی مناسب و مدیریت درمان RTI ها کمک کند. روش کار: 120 نمونه تنفسی از بیماران مبتلا به تظاهرات شبه آنفولانزا از بیمارستان‌های وابسته به دانشگاه علوم پزشکی ایران طی سال‌های 91-91 تهیه شد. استخراج اسید نوکلئیک انجام شد و نمونه‌ها با روش Real-time PCR برای بررسی هفت ویروس تنفسی آنالیز شدند. یافته‌ها: از 120 نمونه تنفسی جمع‌آوری‌شده از بیماران مبتلا به RTI، ویروس‌ها در 41 نمونه (16/34%) شناسایی شدند که شامل 24 (7/40%) زن و 17 (9/27%) مرد بود. SARS-CoV-2 (n=22، 18.3%) و ویروس پاراآنفلوآنزا (n=18، 15%) ویروس‌های تنفسی غالب بودند که در مطالعه ما شناسایی شدند، و پس از آن RSV (n=1، 0.8٪). سایر ویروس های تنفسی مانند متاپنوموویروس، آدنوویروس، بوکاویروس و رینوویروس در مطالعه ما یافت نشدند. ما نتوانستیم هیچ عفونت مشترکی را در تجزیه و تحلیل خود تشخیص دهیم. <div style="text-align: justify;">Introduction: <a name="_Hlk165012230">Respiratory tract infection (RTI) is among the important factors that can threaten human health and is one of the most prevalent etiological agents of death, especially in young children. </a>Viruses account for approximately 80% of various RTIs worldwide. Our study aimed to investigate the molecular prevalence of seven respiratory viruses in RTI. Our results can help develop appropriate public health strategies and treatment management of RTIs. Materials and Methods: 120 respiratory samples were obtained from patients with flu-like manifestations from hospitals referred to the Iran University of Medical Sciences during 2020-2022. Nucleic acid extraction was performed, and then real-time polymerase chain reaction  was performed to analyze the specimens to investigate seven respiratory viruses. Results: Of the 120 respiratory specimens collected from patients with RTI, viruses were identified in 41 (34.16%) samples, encompassing 24 (40.7%) females and 17 (27.9%) males. SARS-CoV-2 (n = 22, 18.3%) and Parainfluenza virus (n=18, 15%) were the predominant detected viruses in the present study, followed by respiratory syncytial viruses (n=1, 0.8%). Other viruses like Metapneumovirus, Adenovirus, Bocavirus, and Rhinovirus were not found in our study. We could not detect any co-infection in our analysis. Conclusion: SARS-CoV-2 and Parainfluenza-virus were the predominant viruses detected in our study, followed by respiratory syncytial viruses. Other viruses, such as Metapneumovirus, Adenovirus, Bocavirus, and Rhinovirus, have not been found in our study. We could not detect any co-infection in our consideration. This result can help develop appropriate public health strategies, targeting prevention and avoiding inappropriate treatment such as using unnecessary antibiotics. </div> عفونت دستگاه تنفسی, ویروس سارس2,ریل تایم پی سی آر, ویروس پاراآنفلوآنزا, ویروس سنسیشیال تنفسی Parainfluenza virus, Real-time PCR, Respiratory syncytial virus, Respiratory tract infection, SARS-CoV-2 45 53 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-503-2&slc_lang=en&sid=1 zahra salavatiha زهرا صلواتیها z.salavatiha96@gmail.com No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Seyed Jalal Kiani سیدجلال کیانی kiani.j@iums.ac.ir 0000-0002-5499-8924 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Ahmad Tavakoli احمد توکلی ahmad.tavakoli66@gmail.com 0000-0003-1857-0610 No Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Mohammad Hadi Karbalaie Niya محمدهادی کربلایی نیا mohamad.karbalai@yahoo.com 0000-0002-1364-5743 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran, Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran, Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran Alireza Javan علیرضا جوان alirezajavan1365@gmail.com No Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Zahra Safaei زهرا صفایی zahra.safaie@hotmail.com No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohamadreza Rezvani محمدرضا رضوانی mohamadrezarezvani574@gmail.com No Department of Hematology and Blood Transfusion, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran Department of Hematology and Blood Transfusion, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran Farah Bokharaei-Salim فرح بخارایی سلیم Bokharaei-Salim@gmail.com 0000-0002-5367-0847 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Seyed Hamidreza Monavari سیدحمیدرضا منوری Monavari.hr@iums.ac.ir 0000-0003-4475-443X Yes Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran