International Journal of Medical Laboratory

en تأثیر روغن جوانه گندم و موسیقی درمانی بر آسیب بیضه ناشی از استرس بی حرکتی حاد و مزمن در موش های صحرایی نر Combined Impact of Wheat Germ Oil and Music Therapy on Testicular Damage Caused by Acute and Chronic Immobility Stress in Male Rats ژنتیک/ بیوتکنولوژی Genetics/ Biotechnology پژوهشي Research <div style="text-align: justify;">Introduction: There is increasing evidence that stress exposure leads to a series of male reproductive system disorders. Wheat germ oil is one of the richest vitamin E and α-tocopherol sources, which have antioxidant properties. Music therapy is appropriate for stress reduction in a variety of mental and medical healthcare centers. This study proposed to evaluate the effect of wheat germ oil and music intervention on testis tissue changes induced by acute and chronic immobility stress in male rats. Materials and Methods: Thirty-five male rats, each weighing 230 ± 20 g, were randomly divided into seven groups: 1) control, 2) acute stress, 3) chronic stress, 4) acute stress + wheat germ oil, 5) chronic stress + wheat germ oil, 6) acute stress + music, and 7) chronic stress + music. Following the intervention period, the rats were euthanized, and blood and testicular tissues were collected. Body weight, sperm parameters, spermatogenesis indices, morphological and morphometric changes, oxidative stress markers, and serum testosterone levels were assessed. Results: Chronic stress led to significant reductions in body weight, sperm parameters (including count, motility, and viability), spermatogenesis indices, and morphometric indices. Additionally, oxidative stress levels increased, while catalase activity and testosterone levels decreased. However, these adverse effects were mitigated in groups treated with wheat germ oil and exposed to music, resulting in the normalization of these parameters. Conclusion: This study reveals that immobility stress enhances testicular damage indices, but the use of wheat germ oil and hearing the music improves these parameter.</div> Immobility stress, Music therapy, Reproductive system, Sperm parameters, Wheat germ oil 162 175 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-549-1&slc_lang=en&sid=1 Vahid Nejati وحید نجاتی v.nejati2022@gmail.com No Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran. Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran Ayshe Hajiesmailpoor عایشه حاجی اسماعیل پور Esmaeilpoor86@gmail.com No Department of Emergency Medical Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran Department of Emergency Medical Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran Rahil Norbakhsh راحیل نوربخش rahilnorbakhsh@yahoo.com No Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Mahsa Zarabadipour مهسا زرآبادی پور zarabadipour.mah@gmail.com No Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Zahra Boroughani زهرا بروغنی boroughani.z@gmail.com No Department of Microbial Biotechnology, University of Tehran, Tehran, Iran Department of Microbial Biotechnology, University of Tehran, Tehran, Iran Sina Dalvand سینا دالوند Sina.dalvand10@gmail.com No International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Amin Namdari امین نامداری a.namdari1990@gmail.com No Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fars, Iran Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fars, Iran Mehdi Shafiee Mehr مهدی شفیعی مهر shafee.mehdi@yahoo.com No School of Medicine, Arak University of Medical Sciences, Arak, Iran School of Medicine, Arak University of Medical Sciences, Arak, Iran Zahra Rabieefar زهرا ربیعی فر zah.rabieefar@gmail.com Yes Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran. Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran