en بررسی پروتئین های ویژه غضروف در کندروژنز سلول های بنیادی مشتق از بافت چربی تحت تاثیر عصاره هسته انار و سویا/ اووکادو در داربست فیبرین عمومى General پژوهشي Research <span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> به دلیل عدم ترمیم ضایعات غضروفی، مهندسی بافت با استفاده از سلول ها، داربست ها و فاکتورهای رشد سعی دارد تا غضروف تولید کند. عصاره هسته انار و سویا/ اووکادو</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">دو ترکیب گیاهی هستند که در حفظ ماتریکس خارج سلولی غضروف موثر هستند.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مواد و روش ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> سلول های بنیادی مشتق از بافت چربی انسانی در پاساژ سوم در سه گروه (شاهد، عصاره هسته انار و سویا/ اووکادو)</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">برای تمایز غضروفی به داربست های فیبرینی منتقل و تقسیم شدند. پس از 14 روز، نمونه ها از نظر بقای سلولی با استفاده از روش </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">MTT</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، تولید پروتئین کلاژن نوع 2 و پروتئین کلاژن نوع 10 توسط تکنیک وسترن بلات بررسی شدند.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نتایج:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> ارزیابی نتایج </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">MTT</span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> در گروه های مختلف نشان داد که میزان بقا در دو گروه عصاره هسته انار و سویا/ اووکادو به طور معنی داری کمتر از گروه کنترل بود (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">P-value &le;0.05</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">).</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نتایج کمی وسترن بلات نشان داد که تولید پروتئین کلاژن نوع 2 در هر دو گروه نسبت به گروه کنترل افزایش معنی داری داشت. تجزیه و تحلیل کمی تولید پروتئین کلاژن نوع 10 نشان داد که تولید این پروتئین در گروه عصاره هسته انار نسبت به گروه سویا/ اووکادو به طور قابل توجهی کاهش یافته است (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">P-value &le;0.05</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نتیجه گیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">عصاره هسته انار و سویا/ اووکادو دو عامل مهم در فرآیند القای کندروژنز در سلول های بنیادی مشتق از بافت چربی در داربست فیبرین هستند. تأثیر سویا/ اووکادو بر غضروف سازی سلول های بنیادی مشتق از بافت چربی در داربست فیبرین بیشتر از تأثیر عصاره هسته انار است.</span></span></span></span></span></span></span></span><br> &nbsp; <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="color:#222222">Introduction:</span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:115%">Due to the lack of repair of cartilage lesions, tissue engineering tries to produce cartilage using cells, scaffolds, and growth factors. Pomegranate seed extract (PSE) and unsaponifiable soybean/ avocado (ASU) are plant compounds that effectively maintain the extracellular cartilage matrix. In this study, we tried to investigate the effects of PSE on the process of chondrogenesis and compared it with the effects of ASU in this process.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Materials and Methods:</span></span></b><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> Human adipose-derived stem cells (hADSCs) were transferred to fibrin scaffolds in the third passage in three groups (control, PSE, and ASU) for chondrogenic differentiation. After 14 days, Western blotting evaluated the samples for cell survival by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay production of collagen type II and collagen type X.</span></span><span style="font-size:10.0pt"><span style="line-height:115%"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="line-height:115%">Results:</span></span></b><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"> Evaluation of MTT results in different groups showed that the survival rate in the two groups of PSE and ASU was significantly lower than the control group (p &le; 0.05). Quantitative western blotting showed that the production of collagen type II protein in both groups significantly increased compared to the control group (p &le; 0.05). Quantitative analysis of collagen type X protein production showed that the production of this protein in the PSE group was significantly reduced compared to the ASU group.</span></span><span style="font-size:10.0pt"><span style="line-height:115%"></span></span></span></span></span><br> <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> PSE and ASU are two important factors in inducing chondrogenesis in hADSCs in fibrin scaffold. ASU&#39;s impact on chondrogenesis of hADSCs in fibrin scaffold is greater than that of PSE.</span></span></div> پروتئین, کلاژن, عصاره هسته انار, آووکادو/سویا, کندروژنز Avocado/Soy, Chondrogenesis, Collagen, Pomegranate Seed Extract, Protein 152 161 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-379-9&slc_lang=en&sid=1 Batool Hashemibeni بتول هاشمی بنی hashemibeni@med.mui.ac.ir No Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mehri Katani مهری کتانی Mehrikatani@gmail.com No Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Behzad Zolfaghari بهزاد ذوالفقاری zolfaghari@pharm.mui.ac.ir No Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Mitra Soleimani میترا سلیمانی soleimani@med.mui.ac.ir No Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Ali Valiani علی والیانی valiani@med.mui.ac.ir No Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Majid Pourentezari مجید پورانتظاری m.pourentezari@gmail.com Yes Department of Biology & Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Yazd Neuroendocrine Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Biology & Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Yazd Neuroendocrine Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran