en - بیوشیمی Biochemistry پژوهشي Research <p>-</p> <p><strong>Backgrounds and Aims:</strong> The purpose of this study was to examine the effects of potassium, sodium and zinc metal ions on ammonium sulfate fraction of acid phosphatase activity of orange fruit.</p> <p><strong>Materials and Methods</strong>: Acid phosphatase was extracted and purified from orange fruit and then various samples as well as a control sample were prepared in vitro. In addition to substrates, ions of potassium, sodium and zinc were added to each sample and then, acid phosphatase activity of each sample was read by spectrophotometer in the determined wavelength.</p> <p><strong>Results and Conclusions:</strong> Zinc, sodium and potassium ions can increase activity of acid phosphatase up to 87.76%, 63.53% and 18.12%, respectively. The results of this study revealed that all three ions of zinc, sodium and potassium can increase acid phosphatase activity.</p> <p></p> Acid phosphatase, Metal ions, Orange fruit 80 85 http://ijml.ssu.ac.ir/browse.php?a_code=A-10-98-1&slc_lang=en&sid=1 Mohammad Aberumand Yes Department of Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Morad Rostami No Department of Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Sareh Aberumand No Department of Medicine, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Masih Namjoonia No Department of Nutrition, Faculty of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Mozhgan Khalafian No Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Iran. Alireza Palangi No Department of Biochemistry, Tarbiat Modares University, Tehran, Iran. Masoomeh Jorfi mas.jorfi@gmail.com No Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.