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microRNAs in T-Cell Acute Lymphoblastic Leukemia: Roles and Contributions to Treatment Response 2 2 T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignancy induced by the proliferation of immature T-cell precursors. Even with the development of multi-agent chemotherapy, treatment failure, and relapse remain the most important challenges because of drug resistance. miRNAs are a class of small non-coding RNAs that modulate the expression of target mRNAs at the post-transcription level. They play significant roles in many biological processes, including tumorigenesis, differentiation, and apoptosis. Recent research has underlined the contribution of specific miRNAs to the pathogenesis of T-ALL and drug resistance. In the present review, the therapeutic potential of miRNA modulation in T-ALL disease will be discussed according to their role in disease biology, mechanisms of resistance, and possible strategies for clinical application. 101 116 2024/10/14 1403/7/23 2024/10/22 1403/8/1 کاوه طاری Kaveh Tari Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Iran k.tari@modares.ac.ir پویا ولی زاده اردلان Pooya Valizadeh Ardalan Graduate Student in Biomedical Sciences, Faculty of Natural Sciences, Bonn- Rhein Sieg University of Applied Sciences, Bonn, Germany Germany pooya@modares.ac.ir نرگس قاسمی مهر Narges Ghasemi Mehr Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ایران ارشیا دارایی Arshia Daraei Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ایران سعید ابرون Saied Abroun Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Iran abroun@modares.ac.ir microRNAs T-cell Acute Lymphoblastic Leukemia Treatment Response [1]. Genescà E, la Starza RJC. Early T-cell precursor ALL and beyond: Immature and ambiguous lineage T-ALL subsets. Blood 2022; 14(8): 1873.##[2]. Tari K, Yarahmadi R, Tabatabaei A, Ahmadi L, Atashi A, Shahjahani M, et al. The role of BCR-ABL P190 in diagnosis and prognosis of aLL patients. Int J Hematol Oncol Stem Cell Res. 2016; 1(3): 118-28.##[3]. Zhang H, Wang H, Qian X, Gao S, Xia J, Liu J, et al. Genetic mutational analysis of pediatric acute lymphoblastic leukemia from a single center in China using exon sequencing. BMC Cancer. 2020; 20(1): 1-11.##[4]. Tari K, Atashi A, Yarahmadi R, Abroun S, Hajifathali A, Kaviani S, et al. Myeloproliferative neoplasms associated with mutation in JAK2V617F and tyrosine kinase inhibitors as therapeutic strategy. J Cancer Res Ther. 2015; 3(2): 1-10.##[5]. Fang H, Wang SA, Beird HC, Tang Z, You MJ, Li S, et al. Morphology, immunophenotype, and suggested diagnostic criteria of TCL1 family–negative T-prolymphocytic leukemia. Haematologica 2024: 75.##[6]. Tari K, Yarahmadi R, Tabatabaei A, Saba F, Abroun S, Atashi A, et al. Myeloproliferative disorders and its associated mutations. Am J Blood Res. 2014; 2(4): 3-11.##[7]. Hefazi M, Litzow MR. Recent advances in the biology and treatment of T cell acute lymphoblastic leukemia. J Hematol Oncol Res. 2018; 13: 265-74.##[8]. Tari K, Nasimian A, Kazi JU, Abroun S. Venetoclax drug increases the apoptosis of T and B acute lymphoblastic leukemia cells by reducing the expression of BCL-2. J Int Med Res. 2023; 12(3): 229.##[9]. Liu Y, Zheng R, Liu Y, Yang L, Li T, Li Y, et al. An easy-to-use nomogram predicting overall survival of adult acute lymphoblastic leukemia. Front Oncol. 2022; 12: 977119.##[10]. Lokuge S, Jayasundara S, Ihalagedara P, Kahanda I, Herath DJB. miRNAFinder: A comprehensive web resource for plant Pre-microRNA classification. Bioinformatics 2022; 215: 104662.##[11]. Dhir A, Dhir S, Proudfoot NJ, Jopling CLJ. Microprocessor mediates transcriptional termination of long non-coding RNA transcripts hosting microRNAs. Nat Struct Mol Biol. 2015; 22(4): 319-27.##[12]. Park JH, Shin CJ. Slicer-independent mechanism drives small-RNA strand separation during human RISC assembly. Nucleic Acids Res. 2015; 43(19): 9418-433.##[13]. Kim J, Yao F, Xiao Z, Sun Y, Ma LJ. MicroRNAs and metastasis: small RNAs play big roles. Cancer Metastasis Rev. 2018; 37: 5-15.##[14]. Ultimo S, Martelli AM, Zauli G, Vitale M, Calin GA, Neri LMJ. Roles and clinical implications of microRNAs in acute lymphoblastic leukemia. J Cell Physiol. 2018; 233(8): 5642-654.##[15]. Deng W, Pan M, Zhu S, Chao R, Wang L. Emerging roles of microRNAs in acute lymphoblastic leukemia and their clinical prospects. Exp Rev Hematol. 2021; 14(11): 987-92.##[16]. Quattrone A, Dassi E. Introduction to bioinformatics resources for post-transcriptional regulation of gene expression. RNA Biol. 2016: 3-28.##[17]. Saki N, Abroun S, Soleimani M, Hajizamani S, Shahjahani M, Kast RE, et al. Involvement of microRNA in T-cell differentiation and malignancy. J Clin Exp Hematopathol. 2015; 9(1): 33.##[18]. Azizidoost S, Nasrolahi A, Sheykhi-Sabzehpoush M, Anbiyaiee A, Khoshnam SE, Farzaneh M, et al. Signaling pathways governing the behaviors of leukemia stem cells. Cell Death Dis. 2024; 11(2): 830-46.##[19]. Belmonte M, Hoofd C, Weng A, Giambra V. Targeting leukemia stem cells: which pathways drive self-renewal activity in T-cell acute lymphoblastic leukemia? Curr Opin Hematol. 2016; 23(1): 34-41.##[20]. Lal PM, Siddiqui MH, Soulat A, Mohan A, Tanush D, Tirath K, et al. MicroRNAs as promising biomarkers and potential therapeutic agents in breast cancer management: a comprehensive review. Crit Rev Oncol Hematol. 2024; 86(6): 3543-50.##[21]. Alsaadi M, Khan MY, Dalhat MH, Bahashwan S, Khan MU, Albar A, et al. Dysregulation of miRNAs in DLBCL: Causative factor for pathogenesis, diagnosis and prognosis. BMC Genomics 2021; 11(10): 1739.##[22]. Fox JM. Repurposing artemisinins for the treatment of acute leukemias: University of Maryland, Baltimore; 2015.##[23]. Shi C, Zhang X, Li X, Zhang L, Li L, Sun Z, et al. Effects of microRNA-21 on the biological functions of T-cell acute lymphoblastic lymphoma/ leukemia. Cancer Biomark. 2016; 12(5): 4173-80.##[24]. Junker F, Chabloz A, Koch U, Radtke FJB, The Journal of the American Society of Hematology. Dicer1 imparts essential survival cues in Notch-driven T-ALL via miR-21–mediated tumor suppressor Pdcd4 repression. Blood. 2015; 126(8): 993-1004.##[25]. Aster JCJB, The Journal of the American Society of Hematology. Dicing up T-ALL. Blood 2015; 126(8): 929-30.##[26]. Shu Y, Wang Y, Lv W-Q, Peng D-Y, Li J, Zhang H, et al. ARRB1-promoted NOTCH1 degradation is suppressed by OncomiR miR-223 in T-cell acute lymphoblastic leukemia. Cell Death Dis. 2020; 80(5): 988-98.##[27]. Ye FJO. MicroRNA expression and activity in T-cell acute lymphoblastic leukemia. J Hematol Oncol. 2018; 9(4): 5445.##[28]. Fragoso AR, Mao T, Wang S, Schaffert S, Min H, Pear WS, Chen CZ. Essential role for Mir-181a1/b1 in T-cell acute lymphoblastic leukemia. Blood 2010; 116(21): 470.##[29]. Yan ZX, Zheng Z, Xue W, Zhao MZ, Fei XC, Wu LL, et al. MicroRNA181a is overexpressed in T‐cell leukemia/lymphoma and related to chemoresistance. Cancer Med. 2015; 2015(1): 197241.##[30]. Chen Z, Stelekati E, Kurachi M, Yu S, Cai Z, Manne S, et al. miR-150 regulates memory CD8 T cell differentiation via c-Myb. Cell Rep. 2017;20(11):2584-597.##[31]. Ménoret A, Agliano F, Karginov TA, Karlinsey KS, Zhou B, Vella A. Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival. Front Immunol. 2023; 14: 1102403.##[32]. Naji P, Heidari MM, Khatami M, Zare-Zardini H, Chamani R. MicroRNAs as a new molecular biomarker for diagnosis and prognosis of T-cell acute lymphoblastic leukemia (T-ALL): a systematic review. Iran J Public Health. 2020; 10(3): 184-99.##[33]. Orçun T, Müge S, Özden HN. MIR223 Gene Silencing via Locked Nucelic Acids in Cell Lines. Journal of Advanced Research in Health Sciences 2020; 3(2): 45-50.##[34]. Mets E, Van der Meulen J, Van Peer G, Boice M, Mestdagh P, Van de Walle I, et al. MicroRNA-193b-3p acts as a tumor suppressor by targeting the MYB oncogene in T-cell acute lymphoblastic leukemia. Leukemia 2015; 29(4): 798-806.##[35]. Bensberg M, Rundquist O, Selimović A, Lagerwall C, Benson M, Gustafsson M, et al. TET2 as a tumor suppressor and therapeutic target in T-cell acute lymphoblastic leukemia. Proceedings of the National Academy of Sciences 2021; 118(34): 2110758118. ##[36]. Correia NG. The role of reciprocal regulation between TAL1 and miRNA expression in T-cell leukemia progression. Seminars in Cancer Biology 2015; 2015(1): 8941471.##[37]. Schotte D, Pieters R, Den Boer ML. MicroRNAs in acute leukemia: from biological players to clinical contributors. Leukemia 2012; 26(1): 1-2. ##[38]. Wade SM, Ohnesorge N, McLoughlin H, Biniecka M, Carter SP, Trenkman M, et al. Dysregulated miR-125a promotes angiogenesis through enhanced glycolysis. EBioMedicine 2019; 47: 402-13. ##[39]. Chao MP, Seita J, Weissman IL. Establishment of a normal hematopoietic and leukemia stem cell hierarchy. Cold Spring Harbor Symposia on Quantitative Biology 2008;73: 439-49. ##[40]. Li W, Wang Y, Liu R, Kasinski AL, Shen H, Slack FJ, Tang DG. MicroRNA-34a: potent tumor suppressor, cancer stem cell inhibitor, and potential anticancer therapeutic. Front Cell Dev Biol. 2021; 9: 640587 [Internet].##[41]. Li L, Yuan L, Luo J, Gao J, Guo J, Xie X, et al. MiR-34a inhibits proliferation and migration of breast cancer through down-regulation of Bcl-2 and SIRT1. Journal of Cellular Biochemistry. 2013; 13: 109-17.##[42]. Neaga A, Bagacean C, Tempescul A, Jimbu L, Mesaros O, Blag C, et al. MicroRNAs associated with a good prognosis of acute myeloid leukemia and their effect on macrophage polarization. Frontiers in Oncology 2021; 11: 582915.##[43]. Chakraborty S, Ghosh Z. MicroRNAs shaping cellular reprogramming. AGO-Driven Non-Coding RNAs. Elsevier; 2019. p. 75-97.##[44]. Pierouli K, Papageorgiou L, Mitsis T, Papakonstantinou E, Diakou I, Leptidis S, et al. Role of microRNAs and long non-coding RNAs in glucocorticoid signaling. Journal of Molecular Endocrinology 2022; 50(6): 147.##[45]. El‐Khazragy N, Elshimy AA, Hassan SS, Matbouly S, Safwat G, Zannoun M, et al. Dysregulation of miR‐125b predicts poor response to therapy in pediatric acute lymphoblastic leukemia. Journal of Cellular Biochemistry 2019; 120(5): 7428-438.##[46]. Singh P. MicroRNA based combinatorial therapy against TKIs resistant CML by inactivating the PI3K/Akt/mTOR pathway: a review. Journal of Molecular Oncology 2023; 40(10): 300.##[47]. da Silva SP, Caires HR, Bergantim R, Guimarães JE, Vasconcelos MH, editors. miRNAs mediated drug resistance in hematological malignancies. Seminars in Cancer Biology. Elsevier; 2022.##[48]. Barbagallo D, Ponti D, Bassani B, Bruno A, Pulze L, Akkihal SA, et al. MiR-223-3p in Cancer development and cancer drug resistance: Same coin, different faces. Journal of Clinical Medicine 2024; 25(15): 8191.##[49]. Grobbelaar C, Ford AM. The role of MicroRNA in paediatric acute lymphoblastic leukaemia: challenges for diagnosis and therapy. Journal of Oncology 2019; 2019(1): 8941471.##[50]. Mondal D, Shinde S, Paul S, Thakur S, Velu G, Tiwari AK, et al. Diagnostic significance of dysregulated miRNAs in T-cell malignancies and their metabolic roles. Frontiers in Cell and Developmental Biology 2023; 13: 1230273.##[51]. Soares-Lima SC, Pombo-de-Oliveira MS, Carneiro FR. The multiple ways Wnt signaling contributes to acute leukemia pathogenesis. Journal of Leukocyte Biology 2020; 108(4): 1081-1099.##[52]. Fan Sj, Li Hb, Cui G, Kong Xl, Sun Ll, Zhao Yq, et al. miRNA-149* promotes cell proliferation and suppresses apoptosis by mediating JunB in T-cell acute lymphoblastic leukemia. Journal of Immunological Methods 2016; 41: 62-70.##[53]. Haftmann C, Riedel R, Porstner M, Wittmann J, Chang HD, Radbruch A, et al. Direct uptake of antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. Journal of Immunological Methods 2015; 426(1): 128-33.##[54]. Barta T, Peskova L, Hampl A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Scientific Reports 2016; 6(1): 36625.##[55]. Lai AY, Sorrentino JA, Dragnev KH, Weiss JM, Owonikoko TK, Rytlewski JA, et al. CDK4/6 inhibition enhances antitumor efficacy of chemotherapy and immune checkpoint inhibitor combinations in preclinical models and enhances T-cell activation in patients with SCLC receiving chemotherapy. J Immunother Cancer. 2020; 8(2): 847.## ##

A Review of COVID-19-associated Fungal Infections 2 2 Acute respiratory coronavirus 2, the causative agent of the 2019 coronavirus disease (COVID-19), has been among the most important pathogens driving the healthcare delivery system in the past few years. Immunosuppressive drugs are used to treat this disease, and perhaps the nature of this disease has increased opportunistic infections in these patients. This study aims to investigate the number of fungal infections in patients with COVID-19. For this reason, keywords such as: “COVID-19”, “Aspergillus”, “fungi”, “Candida”, “Cryptococcus,” “Pneumocystis,” and “Mucorales” were searched. According to the investigation that was carried out, Aspergillus, Cryptococcus, Pneumocystis, Mucorales, and Candida were among the fungi that were investigated in patients with COVID-19. Lung damage, immunosuppression, need for oxygen therapy, steroid therapy, and hospitalization in intensive care units were known predisposing factors for fungal infections in these patients. 117 129 2024/10/142024/07/3 1403/4/13 2024/10/222024/11/12 1403/8/22 علی کمالی Ali Kamali Department of Iinfectious disease, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran ایران mehditaherisarvtin@gmail.com مهدی طاهری سروتین Mehdi Taheri Sarvtin Department of Medical Mycology and Parasitology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran. ایران mehditaheri.mt@gmail.com Coronavirus COVID-19 Fungal Infections SARS-CoV-2 [1]. Guo YR, Cao QD, Hong ZS, Tan YY, Chen SD, Jin HJ, et al. The origin, transmission and clinical therapies on coronavirus disease 2019 (COVID-19) outbreak - an update on the status. Mil Med Res. 2020; 7(1): 11.##[2]. Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, et al. Clinical characteristics of coronavirus disease 2019 in China. N Engl J Med. 2020; 382(18): 1708-720. ##[3]. Omoush SA, Alzyoud JAM. The prevalence and impact of co-infection and superinfection on the severity and outcome of COVID-19 infection: An updated literature review. Pathogens 2022; 11(4): 445.##[4]. Feldman C, Anderson R. The role of co-infections and secondary infections in patients with COVID-19. Pneumonia (Nathan) 2021; 13(1): 5.##[5]. Musuuza JS, Watson L, Parmasad V, Putman-Buehler N, Christensen L, Safdar N. Prevalence and outcomes of co-infection and superinfection with SARS-CoV-2 and other pathogens: A systematic review and meta-analysis. PLoS One 2021; 16(5): 251170. ##[6]. Hughes S, Troise O, Donaldson H, Mughal N, Moore LSP. Bacterial and fungal co-infection among hospitalized patients with COVID-19: a retrospective cohort study in a UK secondary-care setting. Clin Microbiol Infect. 2020; 26(10): 1395-399. ##[7]. Nowak MD, Sordillo EM, Gitman MR, Paniz Mondolfi AE. Co-infection in SARS-CoV-2 infected patients: Where are influenza virus and rhinovirus/enterovirus? J Med Virol. 2020; 92(10): 1699-700. ##[8]. Lansbury L, Lim B, Baskaran V, Lim WS. Co-infections in people with COVID-19: a systematic review and meta-analysis. J Infect. 2020; 81(2): 266-75. ##[9]. Rawson TM, Moore LSP, Zhu N, Ranganathan N, Skolimowska K, Gilchrist M, et al. Bacterial and fungal co-infection in individuals with coronavirus: A rapid review to support COVID-19 antimicrobial prescribing. Clin Infect Dis. 2020; 71(9): 2459-468. ##[10]. Vitale RG, Afeltra J, Seyedmousavi S, Giudicessi SL, Romero SM. An overview of COVID-19 related to fungal infections: what do we know after the first year of pandemic? Braz J Microbiol. 2022; 53(2): 759-75.##[11]. Shishido AA, Mathew M, Baddley JW. Overview of COVID-19-associated invasive fungal infection. Curr Fungal Infect Rep. 2022; 16(3): 87-97. ##[12]. Koehler P, Bassetti M, Chakrabarti A, Chen SCA, Colombo AL, Hoenigl M, et al. Defining and managing COVID-19-associated pulmonary aspergillosis: the 2020 ECMM/ ISHAM consensus criteria for research and clinical guidance. Lancet Infect Dis. 2021; 21(6): 149-62. ##[13]. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al. A Novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020; 382(8): 727-33.##[14]. Shi Y, Wang G, Cai XP, Deng JW, Zheng L, Zhu HH, et al. An overview of COVID-19. J Zhejiang Univ Sci. 2020; 21(5): 343-60.##[15]. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020; 579(7798): 270-73.##[16]. Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, et al. Clinical characteristics of coronavirus disease 2019 in China. N Engl J Med. 2020; 382(18): 1708-720.##[17]. Barek MA, Aziz MA, Islam MS. Impact of age, sex, comorbidities and clinical symptoms on the severity of COVID-19 cases: A meta-analysis with 55 studies and 10014 cases. Heliyon 2020; 6(12): 5684.##[18]. Mokhtari T, Hassani F, Ghaffari N, Ebrahimi B, Yarahmadi A, Hassanzadeh G. COVID-19 and multiorgan failure: A narrative review on potential mechanisms. J Mol Histol. 2020; 51(6): 613-28.##[19]. Mathuria JP, Yadav R, Rajkumar K. Laboratory diagnosis of SARS-CoV-2: A review of current methods. J Infect Public Health. 2020; 13(7): 901-905.##[20]. Zhang R, Ouyang H, Fu L, Wang S, Han J, Huang K, et al. CT features of SARS-CoV-2 pneumonia according to clinical presentation: a retrospective analysis of 120 consecutive patients from Wuhan city. Eur Radiol. 2020; 30(8): 4417-426. ##[21]. Heung LJ, Wiesner DL, Wang K, Rivera A, Hohl TM. Immunity to fungi in the lung. Semin Immunol. 2023; 66: 101728. ##[22]. Taheri Sarvtin MT. A review of the role of indoor fungi in sick building syndrome. Int J Med Lab. 2023; 10(3): 196-207.##[23]. Etxebeste O, Otamendi A, Garzia A, Espeso EA, Cortese MS. Rewiring of transcriptional networks as a major event leading to the diversity of asexual multicellularity in fungi. Crit Rev Microbiol. 2019; 45(5-6): 548-563. ##[24]. Kamali M, Taheri Sarvtin M. A survey on airborne fungal spores in indoor air and outdoor air of Babol city. JJUMS. 2015; 2(1): 116-30.‌##[25]. Kamali M, Taheri Sarvtin M. Fungal colonization of wood and wood products inside the buildings of Sari, northern Iran. SAJEB. 2016; 6(3): 101-104.‌‌##[26]. Taheri Sarvtin M, Hajheydari Z, Hedayati M. A review on the role of fungi in atopic dermatitis. J Maz Univ Med. 2012; 22(87): 115-37.‌##[27]. Taheri Sarvtin M, Hedayati MT, Ayatollahi Mosavi SA, Afsarian MH. An overview on the role of microbial agents in psoriasis. Mazand Univ Med Sci. 23(98): 364-85.##[28]. Taheri Sarvtin M, Shokohi T, Hajheydari Z, Yazdani J, Hedayati MT. Evaluation of candidal colonization and specific humoral responses against Candida albicans in patients with psoriasis. Int J Dermatol. 2014; 53(12): 555-60.##[29]. Taheri Sarvtin M, Hedayati MT, Abastabar M, Shokohi T. Debaryomyces hansenii colonization and its protein profile in psoriasis. Iran J Dermatol. 2014; 17(4):134-37.##[30]. Taheri Sarvtin M, Kamali M, Yazdani J. A review on the risk factors, presentations and treatment of candidemia. JJUMS. 2015; 2(2): 55-60.‌##[31]. Mehni S, Tork Zahrani S, Taheri Sarvtin M, Mojab F, Mirzaei M, Vazirnasab H. Therapeutic effects of bunium perscicum boiss (Black Zira) on candida albicans vaginitis. Biom Pharmacol. 2015; 8(2): 1103-109.‌##[32]. Kamali M, Kamali A, Taheri Sarvti, M. Evaluation of aflatoxin M1 level in human breast milk samples from Jiroft, South of Iran. MLJ. 2020; 14(3): 1-6.##[33]. Kamali M, Seyyedi SS, Taheri Sarvtin M. A study on the presence of aflatoxin M1 in cow’s milk in Jiroft. IJML. 2021; 8(2): 147-53.‌##[34]. Oliveira M, Oliveira D, Lisboa C, Boechat JL, Delgado L. Clinical manifestations of human exposure to fungi. J Fungi (Basel). 2023; 9(3): 381.##[35]. Köhler JR, Hube B, Puccia R, Casadevall A, Perfect JR. Fungi that infect humans. Microbiol Spectr. 5(3): 3-5.‌##[36]. Fisher MC, Gurr SJ, Cuomo CA, Blehert DS, Jin H, Stukenbrock EH, et al. Threats posed by the fungal kingdom to humans, wildlife, and agriculture. MBio. 2020; 11(3): 1110-128.‌##[37]. Salimi M, Davoodi L, Jalalian R, Darayee M, Moslemi A, Faeli L, et al. A fatal Candida albicans pericarditis presenting with cardiac tamponade after COVID-19 infection and cardiothoracic surgery. J Clin Lab Anal. 2023: e24968. ##[38]. Tsang CC, Tang JYM, Lau SKP, Woo PCY. Taxonomy and evolution of Aspergillus, Penicillium and Talaromyces in the omics era - Past, present and future. Comput Struct Biotechnol J. 2018; 16: 197-210.##[39]. Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V, Klaassen CH, et al. Phylogeny, identification and nomenclature of the genus Aspergillus. Stud Mycol. 2014; 78: 141-73. ##[40]. Houbraken J, Kocsubé S, Visagie CM, Yilmaz N, Wang XC, Meijer M, et al. Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species. Stud Mycol. 2020; 95: 155-169.##[41]. Lee JW, Kim SH, You YH, Lim YW, Park MS. Four unrecorded aspergillus species from the rhizosphere soil in South Korea. Mycobiology. 2021; 49(4): 346-354.##[42]. Kocsubé S, Perrone G, Magistà D, Houbraken J, Varga J, Szigeti G, et al. Aspergillus is monophyletic: Evidence from multiple gene phylogenies and extrolites profiles. Stud Mycol. 2016; 85: 199-213.##[43]. Sugui JA, Kwon-Chung KJ, Juvvadi PR, Latgé JP, Steinbach WJ. Aspergillus fumigatus and related species. Cold Spring Harb Perspect Med. 2014; 5(2): a019786. ##[44]. Nuh A, Ramadan N, Schelenz S, Armstrong-James D. Comparative evaluation of MIRONAUT-AM and CLSI broth microdilution method for antifungal susceptibility testing of Aspergillus species against four commonly used antifungals. Med Mycol. 2020; 58(8): 1085-1090. ##[45]. Marr KA, Platt A, Tornheim JA, Zhang SX, Datta K, Cardozo C, Garcia-Vidal C. Aspergillosis complicating severe coronavirus disease. Emerg Infect Dis. 2021; 27(1): 18–25.##[46]. Bartoletti M, Pascale R, Cricca M, Rinaldi M, Maccaro A, Bussini L, et al. Epidemiology of invasive pulmonary aspergillosis among intubated patients with COVID-19: A prospective study. Clin Infect Dis. 2021; 73(11): 3606-614. ##[47]. Lamoth F, Glampedakis E, Boillat-Blanco N, Oddo M, Pagani JL. Incidence of invasive pulmonary aspergillosis among critically ill COVID-19 patients. Clin Microbiol Infect. 2020; 26(12): 1706-708. ##[48]. Van Biesen S, Kwa D, Bosman RJ, Juffermans NP. Detection of invasive pulmonary aspergillosis in COVID-19 with nondirected BAL. Am J Respir Crit Care Med. 2020; 202(8): 1171-173. ##[49]. White PL, Dhillon R, Cordey A, Hughes H, Faggian F, Soni S, et al. A national strategy to diagnose coronavirus disease 2019-associated invasive fungal disease in the intensive care unit. Clin Infect Dis. 2021; 73(7): e1634-e1644.##[50]. Wong S, Fares MA, Zimmermann W, Butler G, Wolfe KH. Evidence from comparative genomics for a complete sexual cycle in the ‘asexual’ pathogenic yeast Candida glabrata. Genome Biol. 2003; 4(2): 10.##[51]. Kamali M, Taheri Sarvtin M. Insights into Candida Albicans: A new perspective on pathogenic factors and regulatory mechanisms. IJML 2023; 10 (2): 91-106.##[52]. Ghazi S, Rafei R, Osman M, El Safadi D, Mallat H, Papon N, et al. The epidemiology of Candida species in the Middle East and North Africa. J Mycol Med. 2019; 29(3): 245-252. ##[53]. Caetano CF, Gaspar C, Martinez-de-Oliveira J, Palmeira-de-Oliveira A, Rolo J. The role of yeasts in human health: A review. Life (Basel) 2023; 13(4): 924. ##[54]. Singh DK, Tóth R, Gácser A. Mechanisms of pathogenic candida species to evade the host complement attack. Front Cell Infect Microbiol. 2020; 10: 94. ##[55]. Arita GS, Conrado PCV, Sakita KM, Rodrigues-Vendramini FAV, Faria DR, Kioshima ES, et al. Serial systemic candidiasis alters Candida albicans macromorphology associated with enhancement of virulence attributes. Microb Pathog. 2022; 164: 105413.##[56]. Froidefond M, Sevestre J, Chaudet H, Ranque S. COVID-19 is a confounder of increased candida airway colonisation. Pathogens 2023; 12(3): 463.##[57]. Samantaray S, Karan P, Sharma A, Nag V, Dutt N, Garg MK, et al. Prevalence, presentation and outcome of secondary bloodstream infections among COVID-19 patients. Infect Disord Drug Targets. 2022; 22(5): 180422203723.##[58]. Karuna T, Garg R, Kumar S, Singh G, Prasad L, Krishen Pandita K, et al. Clinico-epidemio-microbiological exploratory review among COVID-19 patients with secondary infection in central India. Infect Drug Resist. 2022; 15: 1667-1676.##[59]. Jeong S, Lee N, Park Y, Kim J, Jeon K, Park MJ, et al. Prevalence and clinical impact of co-infection in patients with coronavirus disease 2019 in Korea. Viruses 2022; 14(2): 446.##[60]. Tsai CS, Lee SS, Chen WC, Tseng CH, Lee NY, Chen PL, et al. COVID-19-associated candidiasis and the emerging concern of Candida auris infections. J Microbiol Immunol Infect. 2023; 56(4): 672-79.##[61]. Alfaifi A, Sultan AS, Montelongo-Jauregui D, Meiller TF, Jabra-Rizk MA. Long-term post-COVID-19 associated oral inflammatory sequelae. Front Cell Infect Microbiol. 2022; 12: 831744.##[62]. Viciani E, Gaibani P, Castagnetti A, Liberatore A, Bartoletti M, Viale P, et al. Critically ill patients with COVID-19 show lung fungal dysbiosis with reduced microbial diversity in patients colonized with Candida spp. Int J Infect Dis. 2022; 117: 233-40. ##[63]. Lv L, Gu S, Jiang H, Yan R, Chen Y, Chen Y, et al. Gut mycobiota alterations in patients with COVID-19 and H1N1 infections and their associations with clinical features. Commun Biol. 2021; 4(1): 480. ##[64]. Zuo T, Zhan H, Zhang F, Liu Q, Tso EYK, Lui GCY, et al. Alterations in fecal fungal microbiome of patients with COVID-19 during time of hospitalization until discharge. Gastroenterology 2020; 159(4): 1302-1310.##[65]. Li SS, Mody CH. Cryptococcus. Proc Am Thorac Soc. 2010; 7(3): 186-196.‌##[66]. Goughenour KD, Nair AS, Xu J, Olszewski MA, Wozniak KL. Dendritic cells: multifunctional roles in host defenses to cryptococcus infections. J Fungi (Basel). 2023; 9(11): 1050. ##[67]. Macauley P, Epelbaum O. Epidemiology and mycology of candidaemia in non-oncological medical intensive care unit patients in a tertiary center in the United States: Overall analysis and comparison between non-COVID-19 and COVID-19 cases. Mycoses 2021; 64(6): 634-640. ##[68]. Antinori S, Bonazzetti C, Gubertini G, Capetti A, Pagani C, Morena V, et al. Tocilizumab for cytokine storm syndrome in COVID-19 pneumonia: an increased risk for candidemia? Autoimmun Rev. 2020; 19(7): 102564. ##[69]. Burger BJ, Epps SM, Cardenas VM, Jagana R, Meena NK, Atchley WT. Tocilizumab is associated with increased risk of fungal infections among critically Ill patients with COVID-19 and acute renal failure: An observational cohort study. Life (Basel). 2023; 13(8): 1752. ##[70]. Wright TW, Gigliotti F. Pneumocystosis.” diagnosis and treatment of fungal infections. cham: Springer International Publishing, 2023, 237-243.##[71]. Chesnay A, Paget C, Heuzé-Vourc’h N, Baranek T, Desoubeaux G. Pneumocystis pneumonia: pitfalls and hindrances to establishing a reliable animal model. J Fungi (Basel). 2022; 8(2): 129.##[72]. Chong WH, Saha BK, Chopra A. Narrative review of the relationship between COVID-19 and PJP: does it represent co-infection or colonization? Infection. 2021; 49(6): 1079-1090.##[73]. Alanio A, Voicu S, Dellière S, Mégarbane B, Bretagne S. Do COVID-19 patients admitted to the ICU require anti-Pneumocystis jirovecii prophylaxis?. medRxiv2020; 2020-05.‌##[74]. Nicolás FE, Murcia L, Navarro E, Navarro-Mendoza MI, Pérez-Arques C, Garre V. Mucorales species and macrophages. J Fungi (Basel). 2020; 6(2): 94. ##[75]. Wagner L, de Hoog S, Alastruey-Izquierdo A, Voigt K, Kurzai O, Walther G. A revised species concept for opportunistic mucor species reveals species-specific antifungal susceptibility profiles. Antimicrob Agents Chemother. 2019; 63(8): e00653-19.##[76]. Walther G, Wagner L, Kurzai O. Outbreaks of mucorales and the species involved. mycopathologia. 2020; 185(5): 765-81.##[77]. Lynch JP, Fishbein MC, Abtin F, Zhanel GG. Part 1: Mucormycosis: prevalence, risk factors, clinical features, and diagnosis. Expert Rev Anti Infect Ther. 2023; 21(7): 723-36. ##[78]. Sharma B, Nonzom S. Mucormycosis and its upsurge during COVID-19 epidemic: An updated review. Curr Microbiol. 2023; 80(10): 322. ##[79]. Kottarathil M, Thayanidhi P, Sathyamurthy P, Jyoti Kindo A. Rise of mucormycosis during the COVID-19 pandemic and the challenges faced. Curr Med Mycol. 2023; 9(1): 44-55. ##[80]. Chandley P, Subba P, Rohatgi S. COVID-19-associated mucormycosis: A matter of concern amid the SARS-CoV-2 pandemic. Vaccines (Basel). 2022; 10(8): 1266.##[81]. Pal R, Singh B, Bhadada SK, Banerjee M, Bhogal RS, et al. COVID-19-associated mucormycosis: An updated systematic review of literature. Mycoses. 2021; 64(12): 1452-1459.##[82]. Narayanan S, Chua JV, Baddley JW. Coronavirus disease 2019-associated mucormycosis: Risk factors and mechanisms of disease. Clin Infect Dis. 2022; 74(7): 1279-1283.##[83]. Ravindra K, Ahlawat A. Five probable factors responsible for the COVID-associated mucormycosis outbreak in India. Int J Infect Dis. 202; 112: 278-280. ##[84]. Patel A, Agarwal R, Rudramurthy SM, Shevkani M, Xess I, Sharma R, et al. Multicenter epidemiologic study of coronavirus disease–associated mucormycosis, India. Emerg Infect Dis. 2021; 27(9): 2349. ##[85]. Abdoli A, Falahi S, Kenarkoohi A. COVID-19-associated opportunistic infections: a snapshot on the current reports. Clin Exp Med. 2022; 22(3): 327-346.##[86]. Thompson Iii GR, Cornely OA, Pappas PG, Patterson TF, Hoenigl M, et al. Invasive aspergillosis as an under-recognized superinfection in COVID-19. Open Forum Infect Dis. 2020; 7(7): 242.##[87]. Chen G, Wu D, Guo W, Cao Y, Huang D, Wang H, et al. Clinical and immunological features of severe and moderate coronavirus disease 2019. J Clin Invest. 2020; 130(5): 2620-2629.##[88]. Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet 2020; 395(10223): 507-513. ##[89]. Yang X, Yu Y, Xu J, Shu H, Xia J, Liu H, et al. Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study. Lancet Respir Med. 2020; 8(5): 475-481.##[90]. Lescure FX, Bouadma L, Nguyen D, Parisey M, Wicky PH, Behillil S, et al. Lancet Infect Dis. 2020; 20(6): 697-706. ##[91]. Connell NT, Battinelli EM, Connors JM. Coagulopathy of COVID-19 and antiphospholipid antibodies. J Thromb Haemost. 2020; 18(9): 1-2.##[92]. Arastehfar A, Carvalho A, Nguyen MH, Hedayati MT, Netea MG, Perlin DS, et al. COVID-19-Associated Candidiasis (CAC): An Underestimated Complication in the Absence of Immunological Predispositions? J Fungi (Basel). 2020; 6(4): 211.##[93]. Liao M, Liu Y, Yuan J, Wen Y, Xu G, Zhao J, et al. Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19. Nat Med. 2020; 26(6): 842-44.##[94]. Salehi M, Ahmadikia K, Mahmoudi S, Kalantari S, Jamalimoghadamsiahkali S, Izadi A, et al. Oropharyngeal candidiasis in hospitalised COVID-19 patients from Iran: Species identification and antifungal susceptibility pattern. Mycoses. 2020; 63(8): 771-78.##[95]. Kundu R, Singla N. COVID-19 and plethora of fungal infections. Curr Fungal Infect Rep. 2022; 16(2): 47-54.##[96]. Khatib MY, Ahmed AA, Shaat SB, Mohamed AS, Nashwan AJ. Cryptococcemia in a patient with COVID-19: A case report. Clin Case Rep. 2020; 9(2): 853-55. ##[97]. Mina S, Yaakoub H, Annweiler C, Dubée V, Papon N. COVID-19 and Fungal infections: a double debacle. Microbes Infect. 2022; 24(8): 105039.##[98]. Arora U, Priyadarshi M, Katiyar V, Soneja M, Garg P, Gupta I, et al. Risk factors for Coronavirus disease-associated mucormycosis. J Infect. 2022; 84(3): 383-90.## ##

Seroprevalence of Human T-lymphotropic Virus Infections among Pregnant Women in Urmia, Northwest Iran شیوع سرمیHTLV-1/2 در زنان باردار شهرستان ارومیه در سال 1394- 1393 : شمال غرب ایران 2 1 مقدمه و اهداف: ویروس لنفوتروپیک T انسانی HTLV-1/2 میتواند عامل لوسمی و لنفومای سلول های T در بالغین باشدو پاراپارزی اسپاستیک تروپیکال ایجاد کند . هدف مطالعه حاضر تعیین شیوع عفونت HTLV در زنان باردار مراجعه کننده به مراکز بهداشتی درمانی شهرستان ارومیه (شمال غرب ایران) بود.  مواد و روش‌ها: مطالعه مقطعی حاضر در  86 زن باردار ارومیه  در سال 1394  انجام شد.  اطلاعات مرتبط با سایر مورد بررسی در مصاحبه با مادران بارداری جمع اوری شد.  و 5 سی سی نمونه خون از مادران گرفته شده و  از نظر آنتی‌بادی‌های IgG علیه HTLV-1/2 با استفاده از روش‌ELIZA مورد بررسی قرار گرفت. یافته‌ها: میانگین سنی زنان باردار مورد بررسی  30/5 ± 56/25 سال بود،  3 نفر   (49/3% ) سابقه انتقال خون و 18 نفر (93/20 %) سابقه سقط جنین  را گزارش نموده بودند.  هیچ شواهدی از عفونت HTLV-1/2 در جمعیت مورد مطالعه  در آزمایش های سرولوژی رویت نشد. نتیجه‌گیری: در حالی که غربالگری عفونت HTLV-1/2 در زنان باردار اهمیت دارد، به نظر می‌رسد که در طول دوره مطالعه، این موضوع  نمی تواند به عنوان یک نگرانی عمده در سلامت زنان باردار ارومیه  باشد.  با این حال با توجه به اهمیت عفونت با این ویروس در دوران بارداری پیشنهاد می شود غربالگری های دوره ای حتی در مناطق کم خطر انجام شود.   2 Introduction: The human T-lymphotropic virus (HTLV)-1/2 is a retrovirus that can cause adult T-cell leukemia/lymphoma, tissue-necrotizing lymphadenitis, and tropical spastic paraparesis. The purpose of this study was to determine the prevalence of HTLV infection in pregnant women receiving care in Urmia, sited in northwest Iran. Materials and Methods: A cross-sectional study was conducted on 86 pregnant women in Urmia between May and September 2014. Following interviews and blood sample collection, the participants were screened for IgG antibodies against HTLV-1/2 using commercial enzyme-linked immunosorbent assays. Results: The average age of the participants was 25.56 ± 5.30 years, with 3 individuals (3.49%) reporting a history of blood transfusion and 18 patients (20.93%) having experienced previous abortions. Serological testing did not reveal any evidence of HTLV-1/2 infection in the study population. Conclusion: While screening for HTLV-1/2 infection in pregnant women holds significance, it appears that during the study period, this issue was not recognized as a major health concern among pregnant women in Urmia. This observation aligns with findings from other research in Iran, where awareness and emphasis on HTLV-1/2 screening in pregnant populations remain relatively low. 130 135 2024/10/142024/07/32024/06/4 1403/3/15 2024/10/222024/11/122024/11/25 1403/9/5 زکیه رستم زاده خامنه Zakieh Rostamzadeh Khameneh Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran ایران drrostamzadeh@yahoo.com سیما اشنوئی Sima Oshnouei Department of Epidemiology, School of Public Health, Iran University of Medical Sciences, Tehran, Iran iran oshnoyi.sima@gmail.com HTLV Iran Pregnant women Prevalence Serology Urmia کلید واژه : شیوع سرمی HTLV-1/2 عفونت ارومیه [1]. Nazari Z, Ghaffari J, Ghaffari N. Prevalence of Human T lymphotropic virus type 1 in pregnant women: A narrative review. Medical Laboratory Journal 2020; 14(3): 46-8.##[2]. Barmpas D, Monteiro DL, Taquette SR, Trajano AJ, Raupp RM, Miranda FR, Rodrigues NC. Infecção pelo HTLV-1/2 em gestantes brasileiras. Revista Hospital Universitário Pedro Ernesto (HUPE). 2014; 13(3): 81-8.##[3]. Ramos-Rincón JM, Ortiz-Martínez S, Vásquez-Chasnamote ME, de-Miguel-Balsa E, Gamboa-Paredes ON, Talledo-Albujar MJ, et al. Screening for human T-Cell lymphotropic virus (HTLV) in pregnant women in the peruvian amazon and systematic review with meta-analysis of HTLV infection in Peru. Pathogens 2021; 10(3): 260.##[4]. Sánchez-Núñez JP, de-Miguel-Balsa E, Soriano V, Lorenzo-Garrido E, Giménez-Richarte A, Otero-Rodriguez S, et al. Prevalence of HTLV-1/2 infection in pregnant women in Central and South America and the Caribbean: A systematic review and meta‑analysis. Int J Infect Dis. 2024; 143(6): 107018.##[5]. Sampaio GCL, Ribeiro JR, de Almeida CN, Boa-Sorte N, Galvão-Castro B, Grassi MFR, et al. Human T cell lymphotropic virus type 1 global prevalence associated with the human development index: Systematic review with meta-analysis. AIDS Res Hum Retroviruses 2023; 39(4): 145-65.##[6]. Khameneh ZR, Sepehrvand N, Masudi S, Taghizade-Afshari A. Seroprevalence of HTLV-1 among kidney graft recipients: a single-center study. Exp Clin Transplant 2010; 8(2): 146-49.##[7]. Khameneh ZR, Baradaran M, Sepehrvand N. Survey of the seroprovalence of HTLV I/II in hemodialysis patients and blood donors in Urmia. Saudi Journal of Kidney Diseases and Transplantation 2008; 19(5): 838-41.##[8]. Rostamzadeh Z, Valizadeh N, Mohammadian M. Prevalence of seropositivity for human T lymphocytes virus in patients with hereditary bleeding diseases in population of West Azerbaijan. International Journal of Medical Laboratory 2016; 3(3): 159-62.##[9]. Aghamohammadi A, Rafatpanah H, Maghsoodlu M, Tohidi N, Mollahosseini F, Shahabi M. Mannose binding lectin‐associated serine protease 2 (MASP2) gene polymorphism and susceptibility to human T‐lymphotropic virus type 1 (HTLV‐1) infection in blood donors from Mashhad, Iran. Microbiol Immunol. 2022; 66(10): 460-64.##[10]. Ghaffari J, Naghshvar F, Nazari Z, Farid R, Torabizadeh J, Madani F. Seroprevalence of human T-cell lymphotropic virus type 1 infection (HTLV1) in different patients in the north of Iran. Af J Biotechnol. 2011; 10(52): 10752-10755.##[11]. Kalavi Kh, Moradi A, Tabarraei A. Population-based Seroprevalence of HTLV-I Infection in Golestan Province, South East of Caspian Sea, Iran J Basic Med Sci. 2013; 16(3): 225-28.##[12]. Djuicy DD, Mouinga-Ondémé A, Cassar O, Ramassamy JL, Idam Mamimandjiami A, Bikangui R, et al. Risk factors for HTLV-1 infection in Central Africa: A rural population-based survey in Gabon. PLOS Neglect Tropic Dis. 2018; 12(10): 6832.##[13]. Hedayati-Moghaddam, MR, Amini AR. HTLV-1 infection as a serious health issue among Iranian multi-transfused patients: evidence from a systematic review and meta-analysis. Iranian Journal of Blood and Cancer 2015; 7(2): 85-94.## ##

Evaluation of Lipid Peroxidation, Nitric Oxide Metabolites, and Plasma Total Homocysteine Concentration in Patients with Chronic Kidney Disease بررسی پراکسیداسیون لیپیدی، متابولیتهای نیتریک اکسید و غلظت هموسیستئین پلاسما در بیماران مبتلا به نارسایی مزمن کلیه (CKD) 2 1 مقدمه عوامل متعددی در ایجاد آسیب پیشرونده کلیه در نارسایی مزمن کلیه نقش دارند. در این راستا، آسیب اکسیداتیو یکی از عوامل مهم در پیشرفت این بیماری به شمار می آید. اگرچه این مکانیزم کاملا شناخته شده نمی باشد. از اینرو در این مطالعه به بررسی پارامترهای آسیب اکسیداتیو و دیگر پارامترهای بیوشیمیایی مرتبط با آن در مبتلایان به نارسایی مزمن کلیه خواهیم پرداخت. روش ها در این مطالعه مورد – شاهدی ، 60 بیمار مرد مبتلا به نارسایی مزمن کلیه (CKD) با میانگین سنی (14.5±60.2) به عنوان گروه مورد و 60 فرد سالم با میانگین سنی  (9.11±59.9) که سابقه هیچگونه بیماری نظیر ابتلا به فشار خون، دیابت، بیماری های عفونی و بیماری های کلیوی نداشتند به عنوان گروه شاهد انتخاب گردیدند. متغیرهای تحقیق که شامل غلظت مالون دی آلدئید (MDA)، متابولیتهای نیتریک اکسید (نیتریت/نیترات) و غلظت هموسیستئین می شد اندازه گیری شد. نتایج بدست آمده با استفاده از نرم افزارهای آماری آنالیز گردید.   نتایج بر طبق نتایج بدست آمده ، غلظت هموسیستئین در گروه بیمارافزایش معنی داری را نسبت به گروه شاهد نشان می داد (0.05 P<). همچنین غلظت متابولیتهای نیتریت/نیترات در بیماران نسبت به گروه شاهد کاهش یافته بود (0.05 P<). از طرف دیگر، غلظت مالون دی آلدئید (MDA) نیز در گروه بیمار نسبت به گروه شاهد افزایش معنی داری را نشان می داد (0.05 P<). بحث بر طبق نتایج بدست آمده به نظر می رسد که هموسیستئین، پراکسیداسیون لیپیدی همراه با تغییرات نیتریک اکسید می توانند نقش مهمی را در پیشرفت آسیب اکسیداتیو در نارسایی مزمن کلیه  داشته باشد و در پاتوژنز این بیماری دخالت نماید.   2 Introduction: Numerous factors contribute to the advancement of progressive kidney damage in chronic kidney disease (CKD). Among these, oxidative damage plays a significant role in the progression of CKD. The reactions involving free radicals are recognized as a crucial element that can exacerbate oxidative damage in patients with CKD. However, the precise mechanisms underlying oxidative damage remain incompletely understood. Consequently, this study aimed to explore oxidative and other associated biochemical parameters. Materials and Methods: This case-control study included 38 male and 23 female patients, with mean ages of 58.9 ± 15.9 and 62.13 ± 13.43 years, respectively. At the same time, 40 healthy male and 22 healthy female individuals with an average age of 60.33 ± 10.62 and 59.3 ± 6.64, respectively, were selected as the control group who had no history of any diseases such as hypertension, diabetes, infection and kidney disease. Research variables including malondialdehyde (MDA), nitric oxide metabolites (nitrite/nitrate), and homocysteine (Hcy) concentration were measured in both case and control groups. Statistical analysis was done according to the obtained results. Results: There was a significant increase in homocysteine concentration in CKD patients compared to the control (P = 0.001). Nitrite and nitrate metabolites exhibited a substantial reduction in patients when compared to the control group (P = 0.001). On the other hand, malondialdehyde (MDA) showed a significant increase in patients compared to controls (P = 0.01). Conclusion: The findings indicate that homocysteine levels, lipid peroxidation, and alterations in nitric oxide may significantly contribute to the progression of oxidative damage in patients with chronic kidney disease (CKD) and may also influence the disease’s pathogenesis. 136 145 2024/10/142024/07/32024/06/42024/07/3 1403/4/13 2024/10/222024/11/122024/11/252024/11/12 1403/8/22 زینب احمری نژاد Zeynab Ahmarinezhad rsity, Shiraz, Iran ایران faezeh.orris@yahoo.com محمد رضا دیهیم Mohammad Reza Deyhim Iranian Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran ایران mrdeyhim@yahoo.com محبوب لسان پزشکی Mahbub Lesan Pezeshki Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran ایران lessanpezeshki@yahoo.com محمد تقی نجفی Mohammad Taghi Najafi Nephrology Research Center, Tehran University of Medical Sciences, Tehran, Iran ایران motanjf@gmail.com فهیمه خوش نقش Fahimeh Khoshnaghsh Department of Clinical Laboratory Medicine, Shariat Razavi Hospital, Tehran, Iran ایران f.koshnaghsh@yahoo.com Chronic kidney failure Homocysteine Lipid peroxidation Nitric oxide Oxidative damage نارسایی مزمن کلیه آسیب اکسیداتیو هموسیستئین پراکسیداسیون لیپیدی نیتریک اکسید [1]. Ferenbach D.A, Bonventre J.V. Acute kidney injury and chronic kidney disease: From the laboratory to the clinic. Nephrol Ther. 2016; 12 (Suppl 1): 41-8. ##[2]. Ene-Iordache B, Perico N, Bikbov B, Carminati S, Remuzzi A, Perna A, et al. Chronic kidney disease and cardiovascular risk in six regions of the world (ISN-KDDC): a cross-sectional study. Lancet Glob Health. 2016; 4(5): 307-19. ##[3]. Ling XC, 1 and Ko-Lin Kuo KL. Oxidative stress in chronic kidney disease. Renal Replacement Therapy 2018; 4(53): 1-9. ##[4]. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity 2014; 2014(1): 360438. ##[5]. Repetto M, Semprine J, Boveris A. Lipid peroxidation: chemical mechanism, biological implications, and analytical determination. Lipid peroxidation 2012; 1: 3-30.##[6]. Rajul DK, Lalitha1 DL, and Kiranmayi P. A Study of Lipid Profile and Lipid Peroxidation in Chronic Kidney Disease with Special Reference to Hemodialysis. J Clinic Res Bioeth. 2013; 4(1): 1-5. ##[7]. Popolo A, Autore G, Pinto A, Marzocco S. Oxidative stress in patients with cardiovascular disease and chronic renal failure. Free Radical Research 2013; 47(5): 346-56.##[8]. Fritz KS, Petersen D.R. An overview of the chemistry and biology of reactive aldehydes. Free Radical Biol and Medicine 2013; 59: 85-91. ##[9]. Grotto D, Maria LS, Valentini J, Paniz C, Schmitt G, Garcia SC, et al. Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim. Nova 2009; 32(1):169-74. ##[10]. Mian A, Aranke M, Bryan N.S. Nitric Oxide and its Metabolites in the Critical Phase of Illness: Rapid Biomarkers in the Making. The Open Biochemistry Journal 2013; 7(1): 24-32. ##[11]. Jakubowski H. Homocysteine Modification in Protein Structure/Function and Human Disease. Physiol Rev. 2019; 99(1): 555-604. ##[12]. Jakubowski H. Homocysteine in protein structure/function and human disease: Chemical biology of homocysteine-containing proteins: Springer, 2013.##[13]. Deyhim M.R, Khoshnaghsh F. Plasma homocysteine level and risk of thrombosis. J Appl Environ Biol Sci. 2016; 6(2): 189-93##[14]. Martella BM, Veiga1 GRL, Alves BCA, Azzalis LA, Nqueira BC, Gehrkei FS, et al. The importance of homocysteine levels in the prognosis of patients with chronic renal disease and in hemodialysis patients. J Bras Patol Med Lab. 2018; 54(3): 170-76. ##[15]. Abuja PM, Albertini R. Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins.Clin Chim Acta. 2001; 306(1-2): 1-17. ##[16]. Bryan NS, Grisham MB. Methods to detect nitric oxide and its metabolites in biological samples. Free Radical Biol Med. 2007; 43(5): 645-57.##[17]. Rašic S, Rebic D, Hasi S Raši I, Šarac M. Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome. Mediators of Inflammation 2015; 2015(1): 614357.##[18]. Rusu CC, Racasan S, Kacso IM, Moldovan D, Potra A, Patiu IM. Malondialdehyde can predict survival in hemodialysis patients. Clujul Medical. 2016; 89(2): 250-56. ##[19]. Sridhar AVS, Rao PVNS, Sivakumar V, Satish P, Shalini P, Suchitra M, et al. Study of oxidant and antioxidant status in patients with chronic kidney disease. Journal of Clinical and Scientific Research 2018; 7(3): 124-30.##[20]. Bayes B, Pastor MC, Bonal J, Junca J, Romero R. Homocysteine and lipid peroxidation in hemodialysis: role of folinic acid and vitamin E. Nephrol Dial Transplant 2001; 16(11): 2172-175. ##[21]. Olokor AB, Ojogwu IL, Ugbodaga PF. Hyperhomocysteinemia in Chronic Kidney Disease Patients in a Teaching Hospital in Nigeria. British Journal of Medicine and Medical Research 2016; 18(9): 1-7##[22]. M. Carlstrom & M. F. Montenegro. Therapeutic value of stimulating the nitrate–nitrite–nitric oxide pathway to attenuate oxidative stress and restore nitric oxide bioavailability in cardiorenal disease. Journal of Internal Medicine 2019; 285: 2-10. ##[23]. Daenen K, Andries A, Mekahli D, Schepdae A.V, Jouret F, Bammens B. Oxidative stress in chronic kidney disease. Pediatric Nephrology 2019; 34: 975-91.## ##

Liver Involvement in Childhood Cancers: Clinical and Laboratory Insights درگیری کبد در سرطان های کودکان: بینش های بالینی و آزمایشگاهی 2 2 Introduction: Liver involvement is observed in various malignancies and is characterized by abnormal liver function tests, imaging findings, or clinical signs linked to the liver. This study aimed to evaluate hepatic manifestations in childhood malignancies. Materials and Methods: This cross-sectional descriptive study analyzed hepatic manifestations in children under 18 with malignancy admitted to the pediatric oncology ward between April 2016 and April 2020. Results: Among the 130 patients studied, 82 (63%) were male, and 48 (37%) were female. The mean age was 5.9 ± 4.31 years. Patients with hepatoblastoma, lymphoma, and leukemia exhibited the highest rates of abnormal liver enzyme levels. Elevated aspartate aminotransferase  levels were most frequently noted in patients with hepatoblastoma (83.3%), non-Hodgkin’s lymphoma (58.3%), and acute lymphoblastic leukemia (32.7%). Similarly, elevated alanine aminotransferase levels were highest in patients with hepatoblastoma (50%), Hodgkin’s lymphoma (16.7%), and acute myeloblastic leukemia (22.2%). Hepatomegaly was the most common liver-related clinical sign, occurring in 41.5% of patients. Conclusion: The findings indicate that clinical and laboratory liver involvement is highly prevalent among children with malignancies. Such involvement can provide insights into disease progression and play a critical role in treatment planning. 146 151 2024/10/142024/07/32024/06/42024/07/32024/06/26 1403/4/6 2024/10/222024/11/122024/11/252024/11/122025/01/27 1403/11/8 قاسم میری علی اباد Ghasem Miri-Aliabad Department of Pediatrics, Ali Asghar Children’s Hospital, Iran University of Medical Sciences, Tehran, Iran Iran ghmiri1357@gmail.com حسینعلی خراعی Hossein Ali Khazaei Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Iran h_khazaei118@yahoo.com سیدمحمدنصیرالدین طباطبایی Seyed Mohammad Nasiraldin Tabatabaei Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Iran mttaba89@gmail.com علی خواجه Ali Khajeh Children and Adolescents Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Iran dr_khajehneuro@gmail.com زینب نصری نصرابادی Zeinab Nasri Nasrabadi Department of Pediatrics, Ali Asghar Children’s Hospital, Iran University of Medical Sciences, Tehran, Iran Iran zeinab_nasrinasrabadi@yahoo.com ریحانه رضوانی خراشادی زاده Reyhaneh Rezvani Kharashadizadeh Zahedan University of Medical Sciences, Zahedan, Iran Iran reyhanehr.1371@yahoo.com Childhood cancer Hematologic malignancy Hepatic manifestations Liver involvement [1]. Jorjani G, Roshandel G, Taherian MR, Mirbehbahani N, Moaddabshoar L, Ahmadi A, et al. Epidemiology and geographical patterns of common childhood cancers in Iran: Evidence from the National Cancer Registry. Cancer Epidemiol. 2024; 93:102685.##[2]. Michel G, Von Der Weid NX, Zwahlen M, Redmond S, Strippoli M, Kuehni CE, et al. Incidence of childhood cancer in Switzerland: The Swiss childhood cancer registry. Pediatr Blood Cancer 2008; 50(1): 46-51.##[3]. Hassanipour S, Fathalipour M, Delam H, Ghorbani M, Abdzadeh E, Arab-Zozani M, et al . The incidence of childhood cancer in Iran: A systematic review and meta-analysis. Iran J Ped Hematol Oncol. 2019; 9 (3): 193-206. ##[4]. Smith MA, Seibel NL, Altekruse SF, Ries LA, Melbert DL, O'Leary M, et al. Outcomes for children and adolescents with cancer: challenges for the twenty-first century. J Clin Oncol. 2010; 28: 2625-634. ##[5]. Huang FL, Liao EC, Li CL, Yen CY, Yu SJ. Pathogenesis of pediatric B-cell acute lympho-blastic leukemia: Molecular pathways and disease treatments. Oncol Lett. 2020; 20(1): 448-54. ##[6]. Hunger SP, Mullighan CG. Acute lymphoblastic leukemia in children. New Engl J Med. 2015; 373(16): 1541-552.##[7]. Izadi M, Fazel M, Saadat SH, Taheri S. Hepatic involvement by lymphoproliferative disorders post liver transplantation: PTLD. Int Survey Hepatol. 2011; 5(3): 759-66. ##[8]. Leite NP, Kased N, Hanna RF, Brown MA, Pereira JM, Cunha R, et al. Cross-sectional imaging of extranodal involvement in abdominopelvic lymphoproliferative malignancies. Radio Graphics. 2007; 27(6): 1613-634.##[9]. Tomasian A, Sandrasegaran K, Elsayes KM, Shanbhogue A, Shaaban A, Menias CO. Hematologic malignancies of the liver: spectrum of disease. Radiographics 2015; 35(1): 71-86.‌##[10]. Powell N, Rusli F, Hubscher SG, Karanth M, Mutimer D. Adult T-cell leukemia presenting with acute liver failure. Leuk Res. 2006; 30(10): 1315-317.‌##[11]. Davis M L, Hashemi N. Acute liver failure as a rare initial manifestation of peripheral T-cell lymphoma. World J Hepatol. 2010; 2(10): 384-86.‌##[12]. Belgaumi AF, Hudson MM. Childhood acute lymphoblastic leukemia presenting with severe hepatic dysfunction. Med Pediatr Oncol. 2001; 37(2): 142-44.##[13]. Rivet C, Leverger G, Jacquemin E, Bernard O. Acute leukemia presenting as acute hepatitis without liver failure. J Pediatr Gastroenterol Nutr. 2014; 59(5), 640-41.‌##[14]. Shahriari M, Shakibazad N, Haghpanah S, Ghasemi K. Extramedullary manifestations in acute lymphoblastic leukemia in children: a systematic review and guideline-based approach of treatment. American Journal of Blood Research 2020; 10(6): 360.##[15]. Murakami J, Shimizu Y. Hepatic manifestations in hematological disorders. Int J Hepatol. 2013; 2013: 1-13.##[16]. Miri-Aliabad G, Dahmardeh H. Obstructive jaundice due to pancreatic head mass: A rare and unusual presentation of acute myeloid leukemia in children. Turk Arch Pediatr. 2021; 56(5): 530-32.##[17]. Miri-Aliabad G, Asgarzadeh L, Niazi A. Advanced gastric adenocarcinoma in a 12-year-old girl. Iranian Journal of Pediatrics 2021; 31(4): 108870.##[18]. Devarapalli UV, Sarma MS, Mathiyazhagan G. Gut and liver involvement in pediatric hemato-lymphoid malignancies. World J Gastrointest Oncol. 2022; 14(3): 587-606.##[19]. Miri-Aliabad G, Dahmardeh H. Acute pancreatitis as a rare and unusual manifestation of COVID-19 in a child with acute lymphoblastic leukemia. Int J Cancer Manag. 2023; 16(1): 123515.##[20]. Segal I, Rassekh SR, Bond MC, Senger C, Schreiber RA. Abnormal liver transaminases and conjugated hyperbilirubinemia at presentation of acute lymphoblastic leukemia. Pediatr Blood Cancer 2010; 55: 434-39.##[21]. Sharma PB, Karki L. Abnormal hepatic function and splenomegaly on the newly diagnosed acute leukemia patients. J Nepal Med Assoc. 2007; 46(168): 165-69.##[22]. Tsai HJ, Hsieh MY, Tsai YC, Liu ZY, Hsieh HY, Lee CM, et al. Liver function tests may be useful tools for advanced cancer patient care: a preliminary single-center result. Kaohsiung J Med Sci. 2014; 30:146-52. ##[23]. Felice MS, Hammermuller E, De Dávila MT, Ciocca ME, Fraquelli LE, Lorusso AM, et al. Acute lymphoblastic leukemia presenting as acute hepatic failure in childhood. Leuk Lymphoma 2000; 38(5-6): 633-37.## ##

Cartilage-Specific Protein Expression in Adipose-Derived Stem Cells Treated with Pomegranate Seed Extract and Soybean/Avocado in Fibrin Scaffolds بررسی پروتئین های ویژه غضروف در کندروژنز سلول های بنیادی مشتق از بافت چربی تحت تاثیر عصاره هسته انار و سویا/ اووکادو در داربست فیبرین 2 1 مقدمه: به دلیل عدم ترمیم ضایعات غضروفی، مهندسی بافت با استفاده از سلول ها، داربست ها و فاکتورهای رشد سعی دارد تا غضروف تولید کند. عصاره هسته انار و سویا/ اووکادو دو ترکیب گیاهی هستند که در حفظ ماتریکس خارج سلولی غضروف موثر هستند. مواد و روش ها: سلول های بنیادی مشتق از بافت چربی انسانی در پاساژ سوم در سه گروه (شاهد، عصاره هسته انار و سویا/ اووکادو) برای تمایز غضروفی به داربست های فیبرینی منتقل و تقسیم شدند. پس از 14 روز، نمونه ها از نظر بقای سلولی با استفاده از روش MTT، تولید پروتئین کلاژن نوع 2 و پروتئین کلاژن نوع 10 توسط تکنیک وسترن بلات بررسی شدند. نتایج: ارزیابی نتایج MTT در گروه های مختلف نشان داد که میزان بقا در دو گروه عصاره هسته انار و سویا/ اووکادو به طور معنی داری کمتر از گروه کنترل بود (P-value ≤0.05). نتایج کمی وسترن بلات نشان داد که تولید پروتئین کلاژن نوع 2 در هر دو گروه نسبت به گروه کنترل افزایش معنی داری داشت. تجزیه و تحلیل کمی تولید پروتئین کلاژن نوع 10 نشان داد که تولید این پروتئین در گروه عصاره هسته انار نسبت به گروه سویا/ اووکادو به طور قابل توجهی کاهش یافته است (P-value ≤0.05). نتیجه گیری: عصاره هسته انار و سویا/ اووکادو دو عامل مهم در فرآیند القای کندروژنز در سلول های بنیادی مشتق از بافت چربی در داربست فیبرین هستند. تأثیر سویا/ اووکادو بر غضروف سازی سلول های بنیادی مشتق از بافت چربی در داربست فیبرین بیشتر از تأثیر عصاره هسته انار است.   2 Introduction: Due to the lack of repair of cartilage lesions, tissue engineering tries to produce cartilage using cells, scaffolds, and growth factors. Pomegranate seed extract (PSE) and unsaponifiable soybean/ avocado (ASU) are plant compounds that effectively maintain the extracellular cartilage matrix. In this study, we tried to investigate the effects of PSE on the process of chondrogenesis and compared it with the effects of ASU in this process. Materials and Methods: Human adipose-derived stem cells (hADSCs) were transferred to fibrin scaffolds in the third passage in three groups (control, PSE, and ASU) for chondrogenic differentiation. After 14 days, Western blotting evaluated the samples for cell survival by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay production of collagen type II and collagen type X. Results: Evaluation of MTT results in different groups showed that the survival rate in the two groups of PSE and ASU was significantly lower than the control group (p ≤ 0.05). Quantitative western blotting showed that the production of collagen type II protein in both groups significantly increased compared to the control group (p ≤ 0.05). Quantitative analysis of collagen type X protein production showed that the production of this protein in the PSE group was significantly reduced compared to the ASU group. Conclusion: PSE and ASU are two important factors in inducing chondrogenesis in hADSCs in fibrin scaffold. ASU's impact on chondrogenesis of hADSCs in fibrin scaffold is greater than that of PSE. 152 161 2024/10/142024/07/32024/06/42024/07/32024/06/262024/12/2 1403/9/12 2024/10/222024/11/122024/11/252024/11/122025/01/272025/02/16 1403/11/28 بتول هاشمی بنی Batool Hashemibeni Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran hashemibeni@med.mui.ac.ir مهری کتانی Mehri Katani Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran Mehrikatani@gmail.com بهزاد ذوالفقاری Behzad Zolfaghari Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Iran zolfaghari@pharm.mui.ac.ir میترا سلیمانی Mitra Soleimani Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran soleimani@med.mui.ac.ir علی والیانی Ali Valiani Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran valiani@med.mui.ac.ir مجید پورانتظاری Majid Pourentezari Department of Biology & Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Yazd Neuroendocrine Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Iran m.pourentezari@gmail.com Avocado/Soy Chondrogenesis Collagen Pomegranate Seed Extract Protein پروتئین کلاژن عصاره هسته انار آووکادو/سویا کندروژنز [1]. Hunziker EB, Lippuner K, Keel M, Shintani N. An educational review of cartilage repair: precepts & practice–myths & misconceptions–progress & prospects. Osteoarthritis and Cartilage 2015; 23(3): 334-50.##[2]. Theocharis AD, Manou D, Karamanos NK. The extracellular matrix as a multitasking player in disease. The FEBS Journal 2019; 286(15): 2830-869.##[3]. Jiang S, Guo W, Tian G, Luo X, Peng L, Liu S, et al. Clinical application status of articular cartilage regeneration techniques: Tissue-engineered cartilage brings new hope. Stem Cells International 2020; 2020: 5690252.##[4]. Courties A, Kouki I, Soliman N, Mathieu S, Sellam J. Osteoarthritis year in review 2024: Epidemiology and therapy. Osteoarthritis and Cartilage 2024; 32(11): 1397-404.##[5]. Kloppenburg M, Berenbaum F. Osteoarthritis year in review 2019: epidemiology and therapy. Osteoarthritis and Cartilage 2020; 28(3): 242-48.##[6]. Contartese D, Tschon M, De Mattei M, Fini M. Sex specific determinants in osteoarthritis: A systematic review of preclinical studies. International Journal of Molecular Sciences 2020; 21(10): 3696.##[7]. Ali Q, Malik S, Malik A, Hafeez MN, Salman S. Role of modern technologies in tissue engineering. Archives of Neuroscience 2020; 7(1): 90394.##[8]. Chen M, Jiang Z, Zou X, You X, Cai Z, Huang J. Advancements in tissue engineering for articular cartilage regeneration. Heliyon 2024; 10(3): 25400.##[9]. Hashemibeni B, Mardani M, Valiani A, Pourentezari M, Anvari M, Yadegari M, et al. Effects of avocado/soybean on the chondrogenesis of human adipose-derived stem cells cultured on polylactic-co-glycolic acid/ fibrin hybrid scaffold. Journal of Applied Biotechnology Reports 2019; 6(4): 145-50.##[10]. Hashemibeni B, Mardani M, Bahrami M, Valiani A, Mehr MS, Pourentezari M. Comparison of fibrin and PLGA/ fibrin scaffolds for chondrogenesis of human adipose derived stem cells by icariin. J Kerman Univ Med Sci. 2020; 27: 14-23.##[11]. Yuan C, Song W, Jiang X, Wang Y, Li C, Yu W, et al. Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives. Stem Cell Research & Therapy 2024; 15(1): 91.##[12]. Al Kayal T, Losi P, Pierozzi S, Soldani G. A new method for fibrin-based electrospun/ sprayed scaffold fabrication. Scientific Reports 2020; 10(1): 1-4.##[13]. Valiani A, Izadi M, Bahramian H, Esfandiari E, Hashemibeni B. Comparison between the effect of kartogenin and TGFβ3 on chondrogenesis of human adipose-derived stem cells in fibrin scaffold. Bratislavske Lekarske Listy 2017; 118(10): 591-97.##[14]. Endo K, Fujita N, Nakagawa T, Nishimura R. Comparison of the effect of growth factors on chondrogenesis of canine mesenchymal stem cells. Journal of Veterinary Medical Science 2019; 81(8): 1211-218.##[15]. Stevens MM, Marini RP, Martin I, Langer R, Shastri VP. FGF‐2 enhances TGF‐β1‐induced periosteal chondrogenesis. Journal of Orthopaedic Research 2004; 22(5): 1114-119.##[16]. Hashemibeni B, Valiani A, Esmaeli M, Kazemi M, Aliakbari M, Iranpour FG. Comparison of the efficacy of piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds. Iranian Journal of Basic Medical Sciences 2018; 21(2): 212.##[17]. Chen MJ, Whiteley JP, Please CP, Ehlicke F, Waters SL, Byrne HM. Identifying chondrogenesis strategies for tissue engineering of articular cartilage. Journal of Tissue Engineering. 2019; 10: 2041731419842431.##[18]. Lippiello L, Nardo JV, Harlan R, Chiou T. Metabolic effects of avocado/ soy unsaponifiables on articular chondrocytes. Evidence-Based Complementary and Alternative Medicine 2008; 5(2): 191-97.##[19]. Ownby SL, Fortuno LV, Au AY, Grzanna MW, Rashmir-Raven AM, Frondoza CG. Expression of proinflammatory mediators is inhibited by an avocado/ soybean unsaponifiables and epigallocatechin gallate combination. Journal of Inflammation 2014; 11(1): 8.##[20]. Au R, Al-Talib T, Au A, Phan P, Frondoza C. Avocado soybean unsaponifiables (ASU) suppress TNF-α, IL-1β, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular chondrocytes and monocyte/ macrophages. Osteoarthritis and Cartilage 2007; 15(11): 1249-55.##[21]. Babu S, Jayaraman S. An update on β-sitosterol: A potential herbal nutraceutical for diabetic management. Biomedicine & Pharmacotherapy 2020; 131:110702.##[22]. Viuda‐Martos M, Fernández‐López J, Pérez‐Álvarez J. Pomegranate and its many functional components as related to human health: a review. Comprehensive Reviews in Food Science and Food Safety 2010; 9(6): 635-54.##[23]. Miguel MG. Anthocyanins: Antioxidant and/or anti-inflammatory activities. Journal of Applied Pharmaceutical Science 2011; 1(6): 7-15.##[24]. Garbacki N, Angenot L, Bassleer C, Damas J, Tits M. Effects of prodelphinidins isolated from Ribes nigrum on chondrocyte metabolism and COX activity. Naunyn-Schmiedeberg's Archives of Pharmacology 2002; 365(6): 434-41.##[25]. Teimourinejad A, Hashemibeni B, Salehi H, Mostafavi FS, Kazemi M, Bahramian H. Chondrogenic activity of two herbal products; pomegranate fruit extract and avocado/soybean unsaponifiable. Research in Pharmaceutical Sciences 2020; 15(4): 358-66.##[26]. Kabiri A, Esfandiari E, Esmaeili A, Hashemibeni B, Pourazar A, Mardani M. Platelet-rich plasma application in chondrogenesis. Advanced Biomedical Research 2014; 3: 138.##[27]. Esfandiari E, Roshankhah S, Mardani M, Hashemibeni B, Naghsh E, Kazemi M, et al. The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells. Iranian Journal of Basic Medical Sciences 2014; 17(8): 571.##[28]. Ahmed TA, Dare EV, Hincke M. Fibrin: a versatile scaffold for tissue engineering applications. Tissue Engineering Part B: Reviews 2008; 14(2): 199-215.##[29]. Malafaya PB, Silva GA, Reis RL. Natural–origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Advanced Drug Delivery Reviews 2007; 59(4-5): 207-33.##[30]. Yang SH, Wu CC, Shih TTF, Chen PQ, Lin FH. Three‐dimensional culture of human nucleus pulposus cells in fibrin clot: comparisons on cellular proliferation and matrix synthesis with cells in alginate. Artificial Organs 2008; 32(1): 70-3.##[31]. Girandon L, Kregar-Velikonja N, Bozikov K, Barlic A. In vitro models for adipose tissue engineering with adipose-derived stem cells using different scaffolds of natural origin. Folia Biol (Praha). 2011; 57(2): 47-56.##[32]. Hashemibeni B, Pourentezari M, Valiani A, Zamani M, Mardani M. Effect of icariin on the chondrogenesis of human adipose derived stem cells on poly (lactic-co-glycolic) acid/fibrin composite scaffold. Int J Adv Biotech Res. 2017; 8(2): 595-605.##[33]. Rahimi HR, Arastoo M, Ostad SN. A comprehensive review of Punica granatum (pomegranate) properties in toxicological, pharmacological, cellular and molecular biology research. Iranian Journal of Pharmaceutical Research 2012; 11(2): 385.##[34]. Hadipour‐Jahromy M, Mozaffari‐Kermani R. Chondroprotective effects of pomegranate juice on monoiodoacetate‐induced osteoarthritis of the knee joint of mice. Phytother Res. 2010; 24(2): 182-5.## ##

Combined Impact of Wheat Germ Oil and Music Therapy on Testicular Damage Caused by Acute and Chronic Immobility Stress in Male Rats تأثیر روغن جوانه گندم و موسیقی درمانی بر آسیب بیضه ناشی از استرس بی حرکتی حاد و مزمن در موش های صحرایی نر 2 2 Introduction: There is increasing evidence that stress exposure leads to a series of male reproductive system disorders. Wheat germ oil is one of the richest vitamin E and α-tocopherol sources, which have antioxidant properties. Music therapy is appropriate for stress reduction in a variety of mental and medical healthcare centers. This study proposed to evaluate the effect of wheat germ oil and music intervention on testis tissue changes induced by acute and chronic immobility stress in male rats. Materials and Methods: Thirty-five male rats, each weighing 230 ± 20 g, were randomly divided into seven groups: 1) control, 2) acute stress, 3) chronic stress, 4) acute stress + wheat germ oil, 5) chronic stress + wheat germ oil, 6) acute stress + music, and 7) chronic stress + music. Following the intervention period, the rats were euthanized, and blood and testicular tissues were collected. Body weight, sperm parameters, spermatogenesis indices, morphological and morphometric changes, oxidative stress markers, and serum testosterone levels were assessed. Results: Chronic stress led to significant reductions in body weight, sperm parameters (including count, motility, and viability), spermatogenesis indices, and morphometric indices. Additionally, oxidative stress levels increased, while catalase activity and testosterone levels decreased. However, these adverse effects were mitigated in groups treated with wheat germ oil and exposed to music, resulting in the normalization of these parameters. Conclusion: This study reveals that immobility stress enhances testicular damage indices, but the use of wheat germ oil and hearing the music improves these parameter. 162 175 2024/10/142024/07/32024/06/42024/07/32024/06/262024/12/22024/12/1 1403/9/11 2024/10/222024/11/122024/11/252024/11/122025/01/272025/02/162025/01/5 1403/10/16 وحید نجاتی Vahid Nejati Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran. ایران v.nejati2022@gmail.com عایشه حاجی اسماعیل پور Ayshe Hajiesmailpoor Department of Emergency Medical Sciences, Faculty of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran ایران Esmaeilpoor86@gmail.com راحیل نوربخش Rahil Norbakhsh Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ایران rahilnorbakhsh@yahoo.com مهسا زرآبادی پور Mahsa Zarabadipour Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ایران zarabadipour.mah@gmail.com زهرا بروغنی Zahra Boroughani Department of Microbial Biotechnology, University of Tehran, Tehran, Iran ایران boroughani.z@gmail.com سینا دالوند Sina Dalvand International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ایران Sina.dalvand10@gmail.com امین نامداری Amin Namdari Department of Clinical Biochemistry, Fasa University of Medical Sciences, Fars, Iran ایران a.namdari1990@gmail.com مهدی شفیعی مهر Mehdi Shafiee Mehr School of Medicine, Arak University of Medical Sciences, Arak, Iran ایران shafee.mehdi@yahoo.com زهرا ربیعی فر Zahra Rabieefar Department of Histology and Embryology, Faculty of Science, Urmia University, Urmia, Iran. ایران zah.rabieefar@gmail.com Immobility stress Music therapy Reproductive system Sperm parameters Wheat germ oil [1]. Choy JT, Eisenberg ML. Male infertility as a window to health. Fertility and Sterility 2018; 110(5): 810-14.##[2]. Barratt CL, Björndahl L, De Jonge CJ, Lamb DJ, Osorio Martini F, McLachlan R, et al. The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance—challenges and future research opportunities. Human Reproduction Update 2017; 23(6): 660-80.##[3]. Lotti F, Maggi M. Sexual dysfunction and male infertility. Nature Reviews Urology 2018; 15(5): 287-307.##[4]. Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nature Reviews Urology 2017; 14(8): 470-85.##[5]. Hammen C, Kim EY, Eberhart NK, Brennan PA. Chronic and acute stress and the prediction of major depression in women. Depression and Anxiety 2009; 26(8): 718-23.##[6]. Porcelli AJ, Delgado MR. Stress and decision making: effects on valuation, learning, and risk-taking. Current Opinion in Behavioral Sciences 2017; 14: 33-9.##[7]. Musavi H, Abazari O, Barartabar Z, Kalaki-Jouybari F, Hemmati-Dinarvand M, Esmaeili P, et al. The benefits of Vitamin D in the COVID-19 pandemic: biochemical and immunological mechanisms. Archives of Physiology and Biochemistry 2020; 1(1): 1-9.##[8]. Fattah A, Asadi A, Shayesteh MRH, Hesari FH, Jamalzehi S, Abbasi M, et al. Fertility and infertility implications in rheumatoid arthritis; state of the art. Inflammation Research 2020; 69: 721-29.##[9]. Maleki N, Khosh Ravesh R, Salehiyeh S, Faisal Faiz A, Ebrahimi M, Sharbati A, et al. Comparative effects of estrogen and silibinin on cardiovascular risk biomarkers in ovariectomized rats. Gene 2022; 823: 146365.##[10]. Dawson DK. Acute stress-induced (takotsubo) cardiomyopathy. Heart 2018; 104(2): 96-102.##[11]. Van Oort J, Tendolkar I, Hermans E, Mulders P, Beckmann C, Schene A, et al. How the brain connects in response to acute stress: A review at the human brain systems level. Neuroscience & Biobehavioral Reviews 2017; 83(1): 281-97.##[12]. Maleki N, Yavari N, Ebrahimi M, Faiz AF, Ravesh RK, Sharbati A, et al. Silibinin exerts anti-cancer activity on human ovarian cancer cells by increasing apoptosis and inhibiting epithelial-mesenchymal transition (EMT). Gene 2022; 823: 146275.##[13]. Lenow JK, Constantino SM, Daw ND, Phelps EA. Chronic and acute stress promote overexploitation in serial decision making. Journal of Neuroscience 2017; 37(23): 5681-689.##[14]. Mariotti A. The effects of chronic stress on health: new insights into the molecular mechanisms of brain–body communication. Future science OA. 2015; 1(3): 212-20.##[15]. Abazari O, Shafaei Z, Divsalar A, Eslami-Moghadam M, Ghalandari B, Saboury AA, et al. Interaction of the synthesized anticancer compound of the methyl-glycine 1, 10-phenanthroline platinum nitrate with human serum albumin and human hemoglobin proteins by spectroscopy methods and molecular docking. Journal of the Iranian Chemical Society 2020; 17(7): 1601-614.##[16]. Juárez-Rojas AL, García-Lorenzana M, Aragón-Martínez A, Gómez-Quiroz LE, del Socorro Retana-Márquez M. Intrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stress. Systems Biology in Reproductive Medicine 2015; 61(4): 211-21.##[17]. García-Díaz EC, Gómez-Quiroz LE, Arenas-Ríos E, Aragón-Martínez A, Ibarra-Arias JA, Retana-Márquez MDSI. Oxidative status in testis and epididymal sperm parameters after acute and chronic stress by cold-water immersion in the adult rat. Systems Biology in Reproductive Medicine 2015; 61(3): 150-60.##[18]. Sies H. On the history of oxidative stress: Concept and some aspects of current development. Current Opinion in Toxicology 2018; 7(1): 122-26.##[19]. Asadi N, Bahmani M, Kheradmand A, Rafieian-Kopaei M. The impact of oxidative stress on testicular function and the role of antioxidants in improving it: A review. Journal of Clinical and Diagnostic Research 2017; 11(5): 1.##[20]. Durairajanayagam D, Agarwal A, Ong C. Causes, effects and molecular mechanisms of testicular heat stress. Reproductive Biomedicine Online 2015; 30(1): 14-27.##[21]. Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. Journal of Herbmed Pharmacology 2018; 7(1): 1-12.##[22]. Musavi H, Tabnak M, Sheini FA, Bezvan MH, Amidi F, Abbasi M. Effect of garlic (Allium sativum) on male fertility: a systematic review. Journal of Herbmed Pharmacology 2018; 7(4): 306-312.##[23]. Maleki N, Ravesh RK, Salehiyeh S, Faiz AF, Ebrahimi M, Sharbati A, et al. Comparative effects of estrogen and silibinin on cardiovascular risk biomarkers in ovariectomized rats. Gene 2022; 823: 146365.##[24]. Khadangi F, Azzi A. Vitamin E–The next 100 years. IUBMB Life 2019; 71(4): 411-15.##[25]. Abazari O, Divsalar A, Ghobadi R. Inhibitory effects of oxali-Platin as a chemotherapeutic drug on the function and structure of bovine liver catalase. Journal of Biomolecular Structure and Dynamics 2020; 38(2): 609-15.##[26]. Ulfanov O, Cil N, Adiguzel E. Protective effects of vitamin E on aluminium sulphate-induced testicular damage. Toxicology and Industrial Health 2020; 36(4): 215-27.##[27]. Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Etiologies of sperm oxidative stress. International Journal of Reproductive BioMedicine 2016; 14(4): 231-40.##[28]. Alaei Sheini F, Tabnak M, Hasanzadeh Bezvan M, Mahdiannasser M, Musavi H, Choobineh H, et al. A systematic review of the evidence on the effects of cytomegalovirus on abortion. International Journal of Medical Laboratory 2018; 5(3): 173-81.##[29]. Gao J, Chen S, Lin S, Han H. Effect of music therapy on pain behaviors in rats with bone cancer pain. J BUON. 2016; 21(2): 466-72.##[30]. Luo C, Fan H, Li S, Zou Y. Therapeutic of candesartan and music therapy in diabetic retinopathy with depression in rats. Evidence‐Based Complementary and Alternative Medicine 2021; 2021(1): 5570356.##[31]. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 1996; 239(1): 70-6.##[32]. Zahra Z, Tina Nayerpour D, Armaghan L, Zakieh Sadat S, Mohammad P, Fahimeh H, et al. The effect of piperine on MMP-9, VEGF, and E-cadherin expression in breast cancer MCF-7 cell line. Basic & Clinical Cancer Research 2021; 12(3): 232-39.##[33]. Zou P, Wang X, Sun L, Chen Q, Yang H, Zhou N, et al. Semen quality in Chinese college students: associations with depression and physical activity in a cross-sectional study. Psychosomatic Medicine 2018; 80(6): 564-72.##[34]. Mirzaei A, Abbasi M, Sepehri S, Mirzaei M. The effects of Allium porrum and Medicago sativa on iron concentration in thalassemia serums. Life Science Journal 2013; 10(S 11): 27-31.##[35]. Nordkap L, Jensen TK, Hansen ÅM, Lassen TH, Bang AK, Joensen UN, et al. Psychological stress and testicular function: a cross-sectional study of 1,215 Danish men. Fertility and Sterility 2016; 105(1): 174-87.##[36]. de Witte M, Pinho ADS, Stams GJ, Moonen X, Bos AE, van Hooren S. Music therapy for stress reduction: a systematic review and meta-analysis. Health Psychology Review 2022; 16(1): 134-59.##[37]. Shahidi M, Moradi A, Dayati P. Zingerone attenuates zearalenone-induced steroidogenesis impairment and apoptosis in TM3 Leydig cell line. Toxicon 2022; 211: 50-60.##[38]. Hamdi H. The preventive role of wheat germ oil against sertraline‐induced testicular damage in male albino rats. Andrologia 2019; 51(10): 13369.##[39]. Ghafoor K, Özcan MM, AL‐Juhaımı F, Babıker EE, Sarker ZI, Ahmed IAM, et al. Nutritional composition, extraction, and utilization of wheat germ oil: a review. European Journal of Lipid Science and Technology 2017; 119(7): 1600160.##[40]. El-Sayed AI. Effect of wheat germ oil and coenzyme Q10 on physiological performance and testicular oxidative stress markers in rabbit bucks. Annals of Agricultural Science, Moshtohor 2019; 57(1): 47-58.##[41]. Nakata H. Morphology of mouse seminiferous tubules. Anatomical Science International 2019; 94(1): 1-10.##[42]. Pourgholi M, Abazari O, Pourgholi L, Ghasemi-Kasman M, Boroumand M. Association between rs3088440 (G> A) polymorphism at 9p21. 3 locus with the occurrence and severity of coronary artery disease in an Iranian population. Molecular Biology Reports 2021; 48(8): 5905-912.##[43]. Sertoli E. The structure of seminiferous tubules and the development of [spermatids] in rats. Biology of Reproduction 2018; 99(3): 482.##[44]. Zhao X, Wen X, Ji M, Guan X, Chen P, Hao X, et al. Differentiation of seminiferous tubule-associated stem cells into leydig cell and myoid cell lineages. Molecular and Cellular Endocrinology 2021: 111179.##[45]. Zou P, Wang X, Yang W, Liu C, Chen Q, Yang H, et al. Mechanisms of stress-induced spermatogenesis impairment in male rats following unpredictable chronic mild stress (uCMS). International Journal of Molecular Sciences 2019; 20(18): 4470.##[46]. Fukui H, Arai A, Toyoshima K. Efficacy of music therapy in treatment for the patients with Alzheimer’s disease. International Journal of Alzheimer’s Disease 2012; 2012(1): 531646.##[47]. Chung JY, Brown S, Chen H, Liu J, Papadopoulos V, Zirkin B. Effects of pharmacologically induced Leydig cell testosterone production on intratesticular testosterone and spermatogenesis. Biology of Reproduction 2020; 102(2): 489-98.##[48]. Panji M, Behmard V, Zare Z, Malekpour M, Nejadbiglari H, Yavari S, et al. Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines. Gene 2021; 787: 145638.##[49]. Agarwal A, Sengupta P. Oxidative stress and its association with male infertility. Male infertility: Springer, 2020, p. 57-68.##[50]. Beygi Z, Forouhari S, Mahmoudi E, Hayat SM, Nourimand F. Role of oxidative stress and antioxidant supplementation in male fertility. Current Molecular Medicine 2021; 21(4): 265-82.##[51]. Musavi H, Abazari O, Safaee MS, Variji A, Koohshekan B, Kalaki-Jouybari F, et al. Mechanisms of COVID-19 entry into the cell: potential therapeutic approaches based on virus entry inhibition in COVID-19 patients with underlying diseases. Iranian Journal of Allergy, Asthma and Immunology 2021: 1-13.##[52]. Van Tran L, Malla BA, Kumar S, Tyagi AK. Polyunsaturated fatty acids in male ruminant reproduction: A review. Asian-Australasian Journal of Animal Sciences 2017; 30(5): 622.##[53]. Alahmar AT. Role of oxidative stress in male infertility: An updated review. Journal of Human Reproductive Sciences 2019; 12(1): 4.##[54]. Silva LN, Pessoa MTC, Alves SL, Venugopal J, Cortes VF, Santos HL, et al. Differences of lipid membrane modulation and oxidative stress by digoxin and 21-benzylidene digoxin. Experimental Cell Research 2017; 359(1): 291-98.##[55]. Yazar H, Halis F, Nasir Y, Guzel D, Akdogan M, Gokce A. Effect of the oxidant-antioxidant system in seminal plasma on varicocele and idiopathic infertility in male humans. Clinical Laboratory 2017; 63(5): 935-40.##[56]. Ambad RS, Butola MLK, Bankar N, Mahakalkar C. Oxidative stress and anti oxidant status in male infertility. European Journal of Molecular & Clinical Medicine 2021; 8(1): 340-49.##[57]. Barik G, Chaturvedula L, Bobby Z. Role of oxidative stress and antioxidants in male infertility: An interventional study. Journal of Human Reproductive Sciences 2019; 12(3): 204.##[58]. Zare Z, Dizaj TN, Lohrasbi A, Sheikhalishahi ZS, Asadi A, Zakeri M, et al. Silibinin inhibits TGF-β-induced MMP-2 and MMP-9 through Smad Signaling pathway in colorectal cancer HT-29 cells. Basic & Clinical Cancer Research 2020; 12(2): 79-88.##[59]. El-Shiekh R, Al-Mahdy D, Hifnawy M, Abdel-Sattar E. In-vitro screening of selected traditional medicinal plants for their anti-obesity and anti-oxidant activities. South African Journal of Botany. 2019; 123: 43-50.##[60]. Liaqat H, Jeong E, Kim KJ, Kim JY. Effect of wheat germ on metabolic markers: a systematic review and meta-analysis of randomized controlled trials. Food Science and Biotechnology 2020; 29(6): 739-49.## ##

A Bibliometric Study of Articles Published in the International Journal of Medical Laboratory 2 2 Introduction: This study undertakes the first bibliometrics analysis of the past 10 years of the International Journal of Medical Laboratory (IJML), covering the articles published from 2014 to 2023. The study's focus is to describe the characteristics of scientific outputs by analyzing the performance and conceptual trends of IJML. Methods and Materials: This applied study examines all articles published in the IJML journal through all its 32 issues by 2023. Keywords and authors' scientific collaboration network analysis were performed through bibliometrics and social network analysis, using Microsoft Excel, VOSviewer, and statistical software. Results: 288 articles were published in the IJML journal, by 1315 authors from 116 universities and research institutes from 2014-2023. “PCR technique”, “Bacteria,” and “Neoplasms” were the most valuable body of information identified based on the keyword visualization and density maps. There was no significant relationship between title length and abstract views or fulltext downloads (p = 0.514, p = 0.362). However, a significant correlation exists between abstract views and fulltext downloads (p = 0.000). Statistical correlation coefficients for the top 50 organizations show a strong positive correlation among the number of authors, submissions, and acceptances (p = 0.000). Conclusion: The paper identified the leading trends of the IJML journal in terms of papers, authors, institutions, and keywords. There was a moderate variety according to the distribution and scope of the contents published. Analyzing the journal’s trends and current research helps policymakers foster innovation and collaboration in medical laboratory science research. 176 190 2024/10/142024/07/32024/06/42024/07/32024/06/262024/12/22024/12/12024/07/1 1403/4/11 2024/10/222024/11/122024/11/252024/11/122025/01/272025/02/162025/01/52024/07/28 1403/5/7 اسماعیل مصطفوی Ismael Mostafavi Department of Information Science and Knowledge Studies, Yazd University, Yazd, Iran ایران mostafavi@yazd.ac.ir سمیه دهقانی سانیج Somaye Dehghani-Sanij Department of Medical Library and Information Science, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran ایران ijml.office@gmail.com Bibliometrics Medical laboratory journals Periodicals as topic Science mapping analysis Social network analysis [1]. Babyar J. Laboratory science and a glimpse into the future. International Journal of Healthcare Management. 2020; 13(S1): 456-63.##[2]. Javaid M, Haleem A, Singh RP, Suman R, Rab S. Significance of machine learning in healthcare: Features, pillars and applications. International Journal of Intelligent Networks 2022; 3(1): 58-73.##[3]. Greaves RF, Bernardini S, Ferrari M, Fortina P, Gouget B, Gruson D, et al. Key questions about the future of laboratory medicine in the next decade of the 21st century: a report from the IFCC-Emerging Technologies Division. Clinica Chimica Acta 2019; 495: 570-89.##[4]. International Journal of Medical Laboratory [Internet]. Yazd, Iran: Shahid Sadoughi University of Medical Sciences; 2014-2025. [cited 2024 Aug 20]. Available from: https://ijml.ssu.ac.ir/##[5]. Mostafavi I. Analyzing the Collaboration Network of Global Scientific Outputs in the Field of Bibliotherapy in the Web of Science Database. Journal of Payavard Salamat 2022; 15(6): 562-77.##[6]. Tiberius V, Weyland M. Entrepreneurship education or entrepreneurship education? A bibliometric analysis. Journal of Further and Higher Education 2023; 47(1): 134-49.##[7]. Delwiche FA. Mapping the literature of clinical laboratory science. J Med Libr Assoc. 2003; 91(3): 303-10. ##[8]. Moral-Muñoz JA, Herrera-Viedma E, Santisteban-Espejo A, Cobo MJ. Software tools for conducting bibliometric analysis in science: An up-to-date review. Profesional de la Información. 2020; 29(1): 1-20.##[9]. Passas I. Bibliometric analysis: The main steps. Encyclopedia 2024; 4(2): 1014-1025##[10]. Momtaz H, Seifati SM, Tavakol M. Determining the prevalence and detection of the most prevalent virulence genes in Acinetobacter baumannii isolated from hospital infections. International Journal of Medical Laboratory 2015; 2(2): 87-97.##[11]. Rostaei Rad N, Vakili M, Zavar-reza J, Rezaie S, Shirvani AR. The relationship between thyroid hormone levels and body iron status in Iranian hypothyroidism patients. International Journal of Medical Laboratory 2016; 3(3): 176-84.##[12]. Kamalabadi M, Astani A, Nemati F. Anti-viral effect and mechanism of carvacrol on herpes simplex virus type 1. International Journal of Medical Laboratory 2018; 5(2): 113-22.##[13]. Pourmasumi S, Ghasemi N, Talebi AR, Mehrabani M, Sabeti P. The effect of vitamin E and selenium on sperm chromatin quality in couples with recurrent miscarriage. International Journal of Medical Laboratory 2018; 5(1): 1-10.##[14]. Dashti-R MH, Qane MD, Shefaie F, Nazemian Yazdu M, Bagheri SM. Comparative effect of cinnamon essential oil, diclofenac and morphine on acute and chronic pain in mice. International Journal of Medical Laboratory 2016; 3(2): 92-103.##[15]. Hosseini Pour P, Momtaz H, Serajyan AA, Tajbakhsh E. Investigating class I, II and III integrons in multidrug resistance in Pseudomonas aeruginosa isolated from hospital infections in Ahvaz. International Journal of Medical Laboratory 2015; 2(3): 168-76.##[16]. Razi M, Dehghani A, Beigi F, Najminejad H, Vatankhahyazdi K, Agha Ayatollahi M, et al. The peep of nanotechnology in reproductive medicine: A mini-review. International Journal of Medical Laboratory 2015; 2(1): 1-5.##[17]. Gholaminejad F, Javadi M, Karami AA, Alizadeh F, Haghighian HK. Propolis supplementation effects on semen parameters, oxidative stress, inflammatory biomarkers and reproductive hormones in infertile men with asthenozoospermia; a randomized clinical trial. International Journal of Medical Laboratory 2019; 6(1): 21-32.##[18]. Nayeri Chegeni T, Ghaffarifar F, Pirestani M, Dalimi Asl A, Maspi N. Genotyping of Acanthamoeba species isolated from keratitis patients by PCR sequencing methods in Tehran, Iran. International Journal of Medical Laboratory 2019; 6(4): 259-65.##[19]. Ghahramani R, Eidi M, Ahmadian H, Hamidi Nomani M, Abbasi R, Alipour M, et al. Anti-diabetic effect of Portulaca oleracea (purslane) seeds in alloxan-induced diabetic rats. International Journal of Medical Laboratory 2016; 3(4): 282-89.##[20]. Lamovšek N, Klun M. Evaluation of biomedical laboratory performance optimisation using the DEA method. Slovenian Journal of Public Health 2020; 59(3): 172-79.##[21]. Zareivenovel M, Nemati-Anaraki L, Ouchi A, Nourizadeh M, Aghashahi M. Iranian journal of allergy, asthma, and immunology: A bibliometric and altmetric analysis from 2005 to 2022. Iran J Allergy Asthma Immunol. 2024; 23(1): 29-51. ##[22]. Saberi MK, Mokhtari H, Ouchi A, Vakilimofrad H. An altmetrics analysis of the articles published in the medical journal of the Islamic Republic of Iran (1987-2020). Med J Islam Repub Iran. 2021; 35(1): 189.##[23]. Siebers R. Accuracy of references in the New Zealand Journal of Medical Laboratory Science. New Zeal J Med Lab Sci. 1999; 53(2): 4648.##[24]. Watson MM, Perrin R. A comparison of CINAHL and MEDLINE CD-ROM in four allied health areas. Bull Med Libr Assoc. 1994; 82(2): 214-16.##[25]. Železnik D, Blažun Vošner H, Kokol P. A bibliometric analysis of the Journal of Advanced Nursing, 1976-2015. J Adv Nurs. 2017; 73(10): 2407-419.##[26]. Li Y, Wang W, Zhang B, Li L, Zhou D. A bibliometric analysis of exosomes in sepsis from 2004 to 2022. Medicine (Baltimore) 2023; 102(31): 34613.##[27]. Henriksen, D. The rise in co-authorship in the social sciences (1980-2013). Scientometrics 2016; 107: 455-76. ##[28]. Ordoobadi AJ, Perez N, Westfal M, Chang DC, Kelleher C. Social network analysis of authors in scientific journals. Journal of the American College of Surgeons 2019; 229(S4): 164.##[29]. Hamdan W, Alsuqaih H. Research output, key topics, and trends in productivity, visibility, and collaboration in social sciences research on COVID-19: A scientometric analysis and visualization. Sage Open 2024; 14(4): 1-24.##[30]. Chen B, Deng D, Zhong Z, Zhang C. Exploring linguistic characteristics of highly browsed and downloaded academic articles. Scientometrics 2020; 122: 1769-790.##[31]. Long H, Lin X, Li Y. Article titles matter in download and citation metrics: Refining descriptive titles. Journal of Scholarly Publishing 2024; 55(3): 313-36.##[32]. Pottier P, Lagisz M, Burke S, Drobniak SM, Downing PA, Macartney EL, et al. Title, abstract and keywords: a practical guide to maximize the visibility and impact of academic papers. Proceedings 2024; 291(2027): 20241222.## ##