1 2423-3706 Shahid Sadoughi University of Medical Sciences 39 Genetics/ Biotechnology The Peep of Nanotechnology in Reproductive Medicine: A Mini-review Razi Mohammad b Dehghani Ali c Beigi Fahimeh d Najminejad Hamid e Vatankhahyazdi Kazem f Agha Ayatollahi Mohammad g Jebali Ali h b Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. c Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. d Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. e Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. f Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. g Pharmaceutics Research Center, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran h 3Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 1 5 2015 2 1 1 15 11 05 2015 11 05 2015 Nanotechnology has opened a new field in medicine as well as in other sciences. The aim of this study was to seek the capability of nanotechnology for the treatment of various reproductive diseases. In this study, we analyzed all articles about “nanotechnology and reproductive medicine” published in 2000-2015, indexed in Google Scholar, PubMed and Science Direct. This study indicated that nanotechnology has been extensively used for different biomedical applications, e.g. detection, drug delivery, diagnostic imaging, etc. particularly in cancer diagnostics and treatment. Here, the emerging uses of nanotechnology in reproductive medicine and reproductive biology were found. The available evidence regarding the use of nanomaterials as experimental tools for the detection and treatment of reproductive diseases was summarized. Nanoparticles have potential applications in reproductive biology. Treatment and imaging of reproductive system-related cancers can be performed by engineered nanoparticles. Also, some non-cancerous diseases can be treated by nanotechnology, e.g. endometriosis. The benefits and concerns associated with their use in a highly delicate system of reproductive tissues and gametes have been investigated. Nano-based methods are innovative and potentially controversial approaches in the clinical settings and give us the mechanisms underlying reproductive diseases.
38 General The Comparison of Active and Passive Recovery after an Intense Exhaustive Training Session on the Level of Serum Serotonin of Male Runners Babaei Mazreno Alireza i Sharifi Gholamreza j Tollabi Mohammad k i Department of Physiology, Islamic Azad University, Khorasgan Branch, Isfahan, Iran. j Department of Physiology, Islamic Azad University, Khorasgan Branch, Isfahan, Iran. k Department of Psychometrics, Allameh Tabatabaei University, Tehran, Iran. 1 5 2015 2 1 16 20 06 05 2015 06 05 2015 Introduction: The aim of this research was to compare active and passive recovery after a session of intense exhaustive training on the level of serotonin in the serum of the runners. Materials and Methods: In this semi-experimental study, 26 male elite runners were randomly assigned to two groups of active (n=12) and passive (n=14) recovery. Half an hour before the start of the training, 5 ml blood was drawn from the sample and then each subject was tested starting training on treadmill for Bruce test until reaching exhaustion. Immediately and then 10 minutes after the Bruce test, 5 ml of blood was drawn again for measuring serotonin. Results and Conclusions:The mean of serotonin in three steps of before, immediately after and 10 minutes after recovery were respectively 360.83 ± 250.05, 459.67±302.13, 514.5±440.48 ng/ml in the active recovery group and 141.26 ± 92.41, 241.04 ± 104.78, 214.34 ± 120.03 ng/ml in the passive recovery group. There was a significant difference between the amount of serotonin after recovery compared to that of before. However, the type of recovery program after that had no effect on the serum serotonin of the blood of the runners 40 Hematology & Blood Banking Uremia Effect on White Blood Cell Count in Patients with Renal Failure Sarabandi Afsaneh l Manafi Shabestari Rima m Farshi Yadolah n Tabibian Shadi o Dorgalaleh Akbar p Esmaeili Reykande Samira Kia Seyyed Hossein Varmaghani Bijan Rashidpanah Jamal l Department of Nursing, Faculty of Medical Sciences, Islamic Azad University, Zahedan Branch, Zahedan, Iran. m Department of Hematology and Blood Transfusion, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. n Department of Hematology and Blood Transfusion, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. o Department of Hematology and Blood Transfusion, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. p Department of Hematology and Blood Transfusion, Iran University of Medical Sciences, Tehran, Iran Department of Hematology and Blood Transfusion, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. Tehran Heart Centre, Tehran University of Medical Sciences, Tehran, Iran. Department of Hematology and Blood Transfusion, Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. Tehran Heart Centre, Tehran University of Medical Sciences, Tehran, Iran. 1 5 2015 2 1 21 24 11 05 2015 11 05 2015 Introduction: It is believed that uremia causes destruction of white blood cells (WBC) and thus causes leukopenia. Therefore this study had an attempt to assess the effect of uremia on WBC count. Materials and Methods: This case control study was conducted on 120 uremic patients and 100 samples as control group. All samples were examined for determination of urea and creatinine in their serum and complete blood counts were determined. Results and Conclusions: In healthy individuals, the mean value of urea was 14.5±1.9 mg/dL and the mean value of creatinine was 0.9±0.2 mg/dL (male) and 0.66±3.2 mg/dL (female). In the patient group, the mean value of urea was 83±2.4 mg/dL. The mean value of creatinin in male and female were 2.4±1.3 mg/dL and 2.1±1.7 mg/dL respectively. The mean of WBC count in case and control groups were 6.08± 2.24 and 6.17± 2.43x109/L respectively (p=0.71). Our results indicate that uremia cannot change leukocyte count. 41 Immunology Serum levels of IL-17A increase in Asthma but not in accordance with Serum level of IgE and Asthma Severity Mowahedi Masouma Samet Mohammad Zare Fateme Samadi Morteza Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Pulmonology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 1 5 2015 2 1 25 33 11 05 2015 11 05 2015 Background and Aims: Recent evidence suggests that T helper (Th) 17 cells are involved in the emergence of asthma. Th17 cells have a key role in inducing inflammation in asthmatic airways thus Interleukin (IL)-17A, the main cytokine of Th17, contributes to airways inflammation. Materials and Methods: We evaluated the level of IL-17A and total immunoglobulin E (IgE) in sera of 100 asthmatic patients and 81 healthy controls by ELISA to determine how serum concentration of IL-17A is associated with asthma severity. We classified patients into three groups mild (n=28), moderate (n=33) and severe cases (n=39). Results: Respectively, serum IL-17A and IgE concentrations were significantly higher in the asthmatic patients than the control group (p=0.026 and p<0.01). Mean of serum IL-17A and IgE values were 37.73 pg/ml and 39.02 IU/ml in the control group and 68.55 pg/ml and 295.87 IU/ml in the patients group. Nevertheless there were non-significant differences between the three groups of asthmatic patients. Respectively, mean of serum IL-17A and IgE values were 94.17 pg/ml and 255.07 IU/ml in the mild group, 71.29 pg/ml and 271.27 IU/ml in the moderate group, and 47.85 pg/ml and 345.97 IU/ml in the severe group. Moreover, there was no correlation between serum levels of IL-17A and IgE. Conclusions: In this study we found that IL-17A, like IgE, rises in sera of asthmatic patients though in a different manner. IgE increases in serum consistent with disease severity though the increases of IL-17A in serum has an inverse relationship with IgE rising. 42 Hematology & Blood Banking Frequency of FLT3 ITD and FLT3 TKD Mutations in Multiple Myeloma Patients (A Case Control Study) Kooshyar Mohammad Mehdi Sadeghian Mohammad Hadi Keramati Mohammad Reza Rahimi Hossein Shams Seyyede Fatemeh Shakeri Sepideh Ayatollahi Hossein Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Hematology and Blood Banking, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Neonatal Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Department of Hematology and Blood Banking, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Hematology and Blood Banking, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Hematology and Blood Banking, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 1 5 2015 2 1 34 40 11 05 2015 11 05 2015 Background and Aims: Multiple myeloma is a malignant proliferation of plasma cells derived from a single clone. The tumor, its products and the host response lead to organ damages. Some factors that are responsible in its pathogenesis are recognized. As FMS like Tyrosine Kinase 3 receptor (FLT3) mutation has been proved as a determining factor in leukemic patients the goal of this study was to find association of FLT3 internal tandem duplication (ITD) and FLT3 tyrosine kinase domain (TKD), mutations with multiple myeloma. Materials and Methods: This case-control study was conducted on 60 paraffin-embedded bone marrow biopsies (30 multiple myeloma and 30 normal bone marrow specimens) in the pathology departments of Ghaem and Imam Reza hospitals in Mashhad. After sections preparation, DNA was extracted and two PCR reactions were set up for detection of FLT3/ ITD and FLT3/TKD mutations. Results: The Mean age of samples was 64±10 years. No FLT3 mutations were detected in multiple myeloma patients. Conclusions: Our findings showed that FLT3 mutations occurrence seem unusual in multiple myeloma. 43 General Effect of Corn Oil, Olive Oil and Sheep’s and Cow’s Ghee on the Expression of apoB Protein in Syrian Mice’s Intestine and Liver Mohammadi Gholamabbas Azizi Hasan Department of Biochemistry, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Department of Biochemistry, Kerman University of Medical Sciences, Kerman, Iran. 1 5 2015 2 1 41 49 11 05 2015 11 05 2015 Background and Aims: Type and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by human have different compositions. One of the important apolipoprotein is apolipoproteinB ( apoB) .This study was conducted to compare the effect of different nutritious fats on expression of apoB protein. Materials and Methods: For this purpose, 48 Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with10%corn oil, diet with10% olive oil, diet with10% cow ghee), diet with10% sheep ghee, and diet with 2% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen and were frozen at -70ºC. Protein was extracted and the expression of apoB was studied by western blotting. Results: An increase in the expression of intestinal apoB48 was identified in olive oil and cow ghee groups. Hepatic apoB100 expression increased in the cholesterol group compared with the corn oil group. Conclusions: This study indicates that olive oil and cow ghee consumption increase intestinal apoB48 expression. 44 Immunology The Effect of an Intense Anaerobic Exercise Session on Serum Levels of IgG, IgM and IgA in Handball, Volleyball and Climbing Sports Jalili Arman Khazae Hossein Ali anchuli Zari Physiology Department, School of Medicine, Zahedan University of Medical Science, Zahedan, Iran Immunology and hematology Department, School of Medicine, Zahedan University of Medical Science, Zahedan, Iran Physiology Department, University of Ferdosi, Mashhad, Iran 1 5 2015 2 1 50 57 11 05 2015 11 05 2015 Background and Aims: The immune system protects the body against many invasive foreign materials. The aim of this study was to investigate the effect of an intense anaerobic exercise session on serum IgG, IgM and IgA levels in handballists, volleybalists and climbers. Materials and Methods: In this study, 45 professional athletes with the average age of 20-30 years who had participated in the major leagues and the first batch of Zahedan city were enrolled. To assess anaerobic power the 30 second Wingate test was used. Blood samples before, immediately after, and 2h after exercises were collected, and serum levels of immunoglobulins IgG, IgM and IgA were measured by nephelometry method. Results: The results of this study indicated that the level of serum immunoglobulins IgG, IgM ˛ IgA concentration in all three study groups before and after and two hours past exercise did not show significant change (P>0.05). Conclusions: Our findings showed that short anaerobic exercise does not have any effect on the level of immunoglobulins in athletes. 46 Hematology & Blood Banking Evaluation of HIV Indeterminate Confirmatory Tests' Results in Blood Donors in Northeast of Iran Shams Seyyede Fatemeh Arian pour Zahra Molahosseini Foomani Farzad Gholi Noori Hossein Hematology and Blood Banking Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Blood Transfusion Organization Research Center, Mashhad, Iran. Blood Transfusion Organization Research Center, Mashhad, Iran. Blood Transfusion Organization Research Center, Mashhad, Iran. 1 5 2015 2 1 58 64 17 05 2015 17 05 2015 Background and Aims: Human Immunodeficiency virus (HIV) is spreading rapidly among the people worldwide. Infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome. Early HIV detection is dependent on antibody screening against virus by Enzyme Linked Immunosorbent Assay (ELISA). Some confirmatory tests such as Western Blot and Recombinant Immunobloting Assay (RIBA) are used to verify viral infection. Many of the confirmatory test results are indeterminate. The aim of this study was to compare the frequency and patterns of indeterminate results of confirmatory tests in two groups blood donors and patients with high risk behaviors in the northeast of Iran. Materials and methods: It is a cross-sectional study from October 2009 to March 2014, a total number of 1055 serum samples with previous positive HIV ElISA test history were tested in our laboratory, Some by RIBA and some by western blot method. Results: Most of the indeterminate results belonged to blood donors and Western Blot analysis. The most reacting band was P24 in both methods and groups. Conclusions: RIBA assay is more sensitive and reliable than western Blot but it’s necessary to use other supplementary tests with less indistinctive results. It’s necessary to pay attention to HIV glycoprotein reactivity in some methods too. 45 Pathology Effects of Ecstasy on Mouse Cardiac Histopathology, Electrocardiogram and Blood Cell Counts Hosseini-Sharifabad Mohammad Hajimaghsoodi Fatheme Karimzade Ali Hekmatimoghaddam Seyedhossein Esmailidehaj Mansour Binesh Fariba Department of Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Internal Medicine, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Pathology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 1 5 2015 2 1 65 72 17 05 2015 17 05 2015 Background and Aims: Ecstasy or 3, 4-methylenedioxymethamphetamine (MDMA) is a brain stimulant and a hallucinogenic material prepared by chemical changes in amphetamine. The aim of this study was to evaluate the changes induced by this drug in mouse cardiac histopathology, electrocardiogram (ECG) and blood cell counts. Materials and Methods: In this experiment, 3 groups (n=10) of mice were enrolled. Group 1, as control, received placebo. Group 2 mice were given single daily low dose (20 mg/kg/d for 28 days) of intraperitoneal MDMA, and group 3 were given single daily high dose (40 mg/kg/d for 28 days) of intraperitoneal MDMA. An ECG, aVF lead record was obtained, and then a blood sample was taken for complete blood counts and the heart was removed for microscopic study of tissue sections with routine staining. Results: The group 3 showed significant decrease in erythrocyte indices, myocarditis in 7 cases and monocyte infiltration around cardiac myocytes in 6 cases. In group 2, lower degree of myocardial injury was observed, displaying significant increase in QT and QTc durations in ECG. In high dose group, red blood count, hematocrit, mean cell volume and mean corpuscular hemoglobin concentration showed significant changes with comparison control group. Conclusions: Ecstasy can effect on red blood cell index and can lead to anemia. Many monocytes see around of cardiac cell with increase of ventricular depolarization and repolarization can lead to increase of QRS-QT interval. Combination myocarditis with arythmia and increase of sinus tachycardia show change in cardiac function and myocardial structure, cardiac injury due to hypoxia and ischemic can cause of myocardial infarction.