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Volume 7, Issue 2 (May 2020)                   IJML 2020, 7(2): 121-127 | Back to browse issues page

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Gholami Yarahmadi S, Morovvati S, Raam M, Morovvati Z. Frequency of Y Chromosome Microdeletions in Azoospermic and Oligospermic Iranian Infertile Men. IJML. 2020; 7 (2) :121-127
URL: http://ijml.ssu.ac.ir/article-1-340-en.html
Faculty of advanced sciences and technology, Islamic Azad University Tehran Medical Sciences, Tehran, Iran
Abstract:   (777 Views)
Background and Aims: Azoospermia factor (AZF) region of the Y-chromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequencies of Y-chromosome microdeletions in azoospermic and oligospermic Iranian infertile men and compare them with other studies in different ethnic groups.
Materials and Methods: At first, karyotype analysis was performed in 80 infertile men and 30 healthy age-matched counterparts as control group using standard cytogenetic methods. Second, genomic DNA was extracted from all cases and genetic screening was conducted for Y chromosome microdeletions by multiplex polymerase chain reaction for AZF genes on both infertile and control men using 6 STS markers on the long arm of the Y chromosome.
Results: Totally, 49 infertile men were azoospermic and 31 were oligospermic. Y-chromosome microdeletions in the AZFc region were detected in 4 of azoospermic patients. Y-chromosome microdeletions was not detected in any of the oligospermic patients and the control group.
Conclusions: This finding recommends that genetic counseling and screening before starting assisted reproductive techniques such as in vitro fertilisation and intracytoplasmic sperm injection can prevent unnecessary treatment and transmission of genetic defects to offspring.
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Type of Study: Research | Subject: Genetics/ Biotechnology
Received: 2019/11/30 | Published: 2020/05/30

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