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International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Tue, May 7, 2024
[Archive]
Volume 7, Issue 3 (August 2020)
IJML 2020, 7(3): 212-223 |
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F Odo O, C Azubuike N, U Achukwu P, C Ugwu M, H Udeogu C, C Ike O. Effects of Crude Aqueous Extract of Cola Acuminata Pods on the Reproductive System of Male Albino Rats. IJML 2020; 7 (3) :212-223
URL: http://ijml.ssu.ac.ir/article-1-351-en.html
URL: http://ijml.ssu.ac.ir/article-1-351-en.html
Ogechukwu F Odo * 
, Nkiruka C Azubuike , Peter U Achukwu , Michael C Ugwu , Chisom H Udeogu , Ozoemena C Ike
, Nkiruka C Azubuike , Peter U Achukwu , Michael C Ugwu , Chisom H Udeogu , Ozoemena C Ike
Department of Histopathology, Enugu State University Teaching Hospital Parklane, Nigeria 2 Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria
Abstract: (948 Views)
Background and Aims: Cola acuminata pod extract (CAPE) is used in Nigerian traditional medicine to treat low sperm count in males. Hence, this study aimed to investigate the effects of CAPE on the reproductive system of male albino rats.
Materials and Methods: Preliminary acute toxicity testing, phytochemical screening, proximate analysis, and determination of vitamin E concentration were performed on the extract. Three groups (I, II, and III) of 12 rats were used in the study. Each group comprised three batches (A, B, and C) of 4 rats each for different periods (4, 6, and 8 weeks, respectively).
Results: Acute toxicity testing showed that CAPE had an oral lethal dose of 50% (LD50) of >5 g/kg body weight (b.wt) in rats. Vitamin E concentration was 0.511 mg/l. The caudal epididymal sperm count increased marginally from 4 to 6 weeks for rats treated with 800 mg/kg b.wt., but reduced significantly at 8 weeks for both CAPE treatment groups (II and III) (p<0.05) compared with the control group (I). Significant reduction (p<0.05) of sperm motility and serum testosterone levels at 8 weeks were observed. The histology of the reproductive organs revealed evident histo-architectural changes.
Conclusions: These results suggest that the aqueous pod extract of Cola acuminata causes marked alterations in reproductive organs and shows antispermatogenic and antiandrogenic effects when administered orally over 8 weeks in mature male rats leading to contradicting its use as a traditional remedy for low sperm count in males.
Materials and Methods: Preliminary acute toxicity testing, phytochemical screening, proximate analysis, and determination of vitamin E concentration were performed on the extract. Three groups (I, II, and III) of 12 rats were used in the study. Each group comprised three batches (A, B, and C) of 4 rats each for different periods (4, 6, and 8 weeks, respectively).
Results: Acute toxicity testing showed that CAPE had an oral lethal dose of 50% (LD50) of >5 g/kg body weight (b.wt) in rats. Vitamin E concentration was 0.511 mg/l. The caudal epididymal sperm count increased marginally from 4 to 6 weeks for rats treated with 800 mg/kg b.wt., but reduced significantly at 8 weeks for both CAPE treatment groups (II and III) (p<0.05) compared with the control group (I). Significant reduction (p<0.05) of sperm motility and serum testosterone levels at 8 weeks were observed. The histology of the reproductive organs revealed evident histo-architectural changes.
Conclusions: These results suggest that the aqueous pod extract of Cola acuminata causes marked alterations in reproductive organs and shows antispermatogenic and antiandrogenic effects when administered orally over 8 weeks in mature male rats leading to contradicting its use as a traditional remedy for low sperm count in males.
Type of Study: Research |
Subject:
General
Received: 2020/03/11 | Accepted: 2020/08/31 | Published: 2020/08/27
Received: 2020/03/11 | Accepted: 2020/08/31 | Published: 2020/08/27
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