Volume 3, Number 1 (February 2016)                   IJML 2016, 3(1): 13-19 | Back to browse issues page


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Jebali A, Kazemi B, Hekmatimoghaddam S. Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles. IJML. 2016; 3 (1) :13-19
URL: http://ijml.ssu.ac.ir/article-1-84-en.html

Abstract:   (439 Views)

Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs).

Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin (βHCG) in the clinical samples was quantified by traditional enzyme-linked immunosorbent assay (ELISA), and then checked by the nano-immunoassay.

Results: The Pearson’s correlation coefficient between ELISA and nano-immunoassay was high, i.e., 0.80. Relative sensitivity and specificity of this nano-immunoassay were reported 97.4% and 96.6%, respectively.

Conclusions: BSA NPs can be applied in nano-immunoassays as a new structure, and as an example, βHCG can be detected by this novel assay.

Full-Text [PDF 159 kb]   (205 Downloads)    
Type of Study: Research | Subject: Genetics/ Biotechnology
Received: 2016/03/9 | Accepted: 2016/03/9 | Published: 2016/03/9

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