Volume 7, Issue 4 (November 2020)                   IJML 2020, 7(4): 280-288 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Teimouri M, Hashemibeni B, Mardani M, Moradi M. Which Culture System Is better for Chondrogenesis of Adipose-Derived Stem Cells ; Pellet or Micromass?. IJML. 2020; 7 (4) :280-288
URL: http://ijml.ssu.ac.ir/article-1-357-en.html
Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract:   (289 Views)
Background and Aims: The current study was conducted to compare the expression levels of collagen type Π and X during chondrogenesis of human adipose-derived mesenchymal stem cells (hADMSCs) pellet and micromass cultures. 
Materials and Methods: Extracted hADMSCs were cultured until three passages and then transferred to pellet and micromass cultures in the experimental groups of day 7 and day14. For pellet and micromass cultures, aliquots of 5×105 cells/ml were centrifuged and respectively cultured in the conical tubes and droplets (12.5 µl) of the 24-well plates containing chondrogenic medium. Realtime-polymerase chain reaction technique was performed for gene expression levels.
Results: Increased expression of collagen type Π was shown in micromass day14 compared to micromass day 7, pellet day 14 (p<0.01) and pellet day 7 (p<0.001). Also, an increased expression of collagen type Π was seen in micromass day 7 and pellet day 14 compared to pellet day 7 (p< 0.05). Expression of collagen type X increased in pellet day 14 compared to micromass on days 7 and 14 (p<0.001, p<0.01) and pellet day14 compared to pellet day7 (p< 0.05). An increased expression of collagen type X was shown in pellet day 7 compared to micromass on days 7 and 14 (p<0.05).
Conclusions: According to the results, higher expression of collagen type Π and lower expression of collagen type X in micromass cultures that are prepared by cell suspension play a better role during cellular condensation that leads to the formation of large nodules exhibiting cartilage-like morphology, suggests a higher efficiency for micromass cultures.
Full-Text [PDF 498 kb]   (109 Downloads) |   |   Full-Text (HTML)  (110 Views)  
Type of Study: Research | Subject: General
Received: 2020/04/21 | Accepted: 2020/09/23 | Published: 2020/11/30

Add your comments about this article : Your username or Email:

Send email to the article author

© 2021 CC BY-NC 4.0 | International Journal of Medical Laboratory

Designed & Developed by : Yektaweb