Write your message
Volume 8, Issue 4 (November 2021)                   IJML 2021, 8(4): 275-283 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Maleki S, Soleimanjahi H, Abdoli A, Karimi H, Banijamali R S, Bamdad T. The Effect of Autophagy Induction in Oncolytic Reovirus Replication in Mesenchymal Stem Cells. IJML 2021; 8 (4) :275-283
URL: http://ijml.ssu.ac.ir/article-1-401-en.html
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (743 Views)
Background and Aims: Oncolytic reoviruses can infect and kill malignant cells while sparing their normal counterparts. Reoviral infection can induce or activate autophagy, even though metformin can induce autophagy. Identifying and regulating the cellular pathways important for reovirus replication and oncolysis can improve targeted-biological therapies for cancer. Here, the autophagic process was triggered via metformin, and we investigated the effect of autophagy activation on oncolytic reovirus replication in mesenchymal stem cells as primary cells and L929 cell lines.
Materials and Methods: Adipose derived mesenchymal stem cells (AD-MSCs) and L929 cells were treated with metformin and reovirus type-3 strain Dearing (T3D). Twenty-four hours after infection, the viability of AD-MSCs and L929 cells were examined by MTT assay. Also, the effect of metformin-induced autophagy in the reovirus replication in these cells was determined by real-time polymerasechain-reaction.
Results: Our results show that treatment with metformin and reovirus reduced the viability of the cells compared to treatment with metformin or reovirus alone in both cells. Also, coadministration of metformin and reovirus significantly decreased the relative expression level of the Beclin-1 gene compared to treatment with metformin in both cells. However, the expression level of the reovirus L3 gene after treatment with metformin and reovirus in L929 cells increased significantly compared to AD-MSCs.
Conclusion: Our data suggest that metformin-induced autophagy enhances reoviral replication in AD-MSCs and L929 cells. These findings represent the role of autophagy induction in facilitating reovirus replication and contribute to a better understanding of reovirus-host interactions.
Full-Text [PDF 266 kb]   (282 Downloads) |   |   Full-Text (HTML)  (331 Views)  
Type of Study: Research | Subject: Virology
Received: 2021/02/11 | Accepted: 2021/07/25 | Published: 2021/12/15

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | International Journal of Medical Laboratory

Designed & Developed by : Yektaweb