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Volume 9, Issue 3 (August 2022)                   IJML 2022, 9(3): 187-197 | Back to browse issues page

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Marjani A, Hesamizadeh K, Bokharaei-Salim F, Khanaliha K, Karbalaie Niya M H, Habib Z et al . Distribution of Epstein-Barr Virus and Human Herpesvirus 8 Co-Infections among Human Immunodeficiency Virus -1 Positive Patients. IJML 2022; 9 (3) :187-197
URL: http://ijml.ssu.ac.ir/article-1-435-en.html
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (598 Views)
Background and Aims: Among Human Immunodeficiency Virus (HIV)-infected individuals, Epstein-Barr virus (EBV) and Human Herpesvirus (HHV)-8 could cause significant illness as opportunistic infections. The purpose of the present study was to evaluate the prevalence of EBV and HHV-8 in saliva specimens obtained from HIV-1 infected Iranian individuals under the Highly Active Antiviral Therapy (HAART) regimen compared with naïve patients.
Materials and Methods: A cross-sectional study was conducted on 103 HIV-1 positive patients who attended the hospitals affiliated with the Iran University of Medical Sciences, in Tehran, Iran, from 2018 to 2019. Enzyme-linked immunosorbent assay (ELISA) test was performed to evaluate HHV-8 and EBV antibodies. A conventional polymerase chain reaction (PCR) was carried out on saliva samples to detect EBV infection and a nested-PCR assay for HHV-8 infection. SPSS (version 20) was used for statistical analysis.
Results: Patients' mean age ± SD was 43.9 ± 16 (range 18-82 years), and from among 103 participants, 59 (57.3%) were male. The results of PCR showed that HHV-8 infection was found in 19 (18.4%), and EBV infection was found in 61 (59.2%) participants. Also, HHV-8 antibody was detected in 73 (70.9%), and EBV antibody in 97 (94.2%) patients. A significant association was observed between patients under treatment with HAART and HHV-8 DNA or EBV DNA infection in saliva.
Conclusions: HIV-infected patients demonstrated a remarkable rate of EBV and HHV-8 in saliva, which could have a great role in the shedding of viruses. Also, they may contribute to the establishment of further opportunistic infections and devastating complications.
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Type of Study: Research | Subject: Virology
Received: 2021/10/10 | Accepted: 2022/08/16 | Published: 2022/10/15

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