International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Thu, Feb 26, 2026
[Archive]
Seyed Amir Mohaghegh 
, Mohammadamin Edalatmanesh , Masoumeh Golestan Jahromi , Ali Aliyan , Aliyar Pirouzi , Mehdi Mohsenzadeh *
, Mohammadamin Edalatmanesh , Masoumeh Golestan Jahromi , Ali Aliyan , Aliyar Pirouzi , Mehdi Mohsenzadeh *
Gerash Al-Zahra Fertility Center, Gerash University of Medical Sciences, Gerash, Iran
Abstract: (12 Views)
Introduction: Approximately 30% of infertile men experience unexplained infertility, a condition categorized as idiopathic. Several studies have reported a correlation between specific genetic polymorphisms and impaired spermatogenesis in infertile men with idiopathic oligoasthenoteratozoospermia. Genetic factors contribute to 60% of cases categorized as idiopathic. Assessing semen quality serves as a crucial criterion in evaluating male fertility.
Materials and Methods: Sampling was conducted at the infertility centre, involving 60 azoospermic patients and 60 healthy individuals. The objective was to investigate and compare the polymorphism status of both groups using polymerase chain reaction and restriction fragment length polymorphism (RFLP) Technique.
Results: In the control group, the highest frequency of GSTP1 gene polymorphisms was observed in individuals with the AA genotype (healthy homozygous) at a rate of 50%. Conversely, the lowest frequency of GSTP1 gene polymorphism in the control group was associated with the G/G genotype (patient homozygous) at a rate of 15 %. Regarding the patient group, the frequency of GSTP1 gene polymorphisms was found to be related to the A/A genotype (healthy homozygous) at 50%. The lowest frequency of GSTP1 gene polymorphism in the patient group was observed in individuals with the G/G genotype (patient homozygous) at 16.6%. Additionally, within this group, the frequency of patient heterozygotes (A/G) was determined to be 33.4%.
Conclusion: The polymorphism study showed no significant disparity in the frequency of two dominant alleles and mutant alleles between the patient and control groups within the population of infertile Iranian men.
Materials and Methods: Sampling was conducted at the infertility centre, involving 60 azoospermic patients and 60 healthy individuals. The objective was to investigate and compare the polymorphism status of both groups using polymerase chain reaction and restriction fragment length polymorphism (RFLP) Technique.
Results: In the control group, the highest frequency of GSTP1 gene polymorphisms was observed in individuals with the AA genotype (healthy homozygous) at a rate of 50%. Conversely, the lowest frequency of GSTP1 gene polymorphism in the control group was associated with the G/G genotype (patient homozygous) at a rate of 15 %. Regarding the patient group, the frequency of GSTP1 gene polymorphisms was found to be related to the A/A genotype (healthy homozygous) at 50%. The lowest frequency of GSTP1 gene polymorphism in the patient group was observed in individuals with the G/G genotype (patient homozygous) at 16.6%. Additionally, within this group, the frequency of patient heterozygotes (A/G) was determined to be 33.4%.
Conclusion: The polymorphism study showed no significant disparity in the frequency of two dominant alleles and mutant alleles between the patient and control groups within the population of infertile Iranian men.
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