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International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Mon, Mar 10, 2025
[Archive]
Batool Hashemibeni 
, Mehri Katani , Behzad Zolfaghari , Mitra Soleimani , Ali Valiani , Majid Pourentezari *
, Mehri Katani , Behzad Zolfaghari , Mitra Soleimani , Ali Valiani , Majid Pourentezari *
Department of Biology & Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Yazd Neuroendocrine Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract: (10 Views)
Introduction: Due to the lack of repair of cartilage lesions, tissue engineering tries to produce cartilage using cells, scaffolds, and growth factors. Pomegranate seed extract (PSE) and unsaponifiable soybean/ avocado (ASU) are plant compounds that effectively maintain the extracellular cartilage matrix. In this study, we tried to investigate the effects of PSE on the process of chondrogenesis and compared it with the effects of ASU in this process.
Materials and Methods: Human adipose-derived stem cells (hADSCs) were transferred to fibrin scaffolds in the third passage in three groups (control, PSE, and ASU) for chondrogenic differentiation. After 14 days, Western blotting evaluated the samples for cell survival by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay production of collagen type II and collagen type X.
Results: Evaluation of MTT results in different groups showed that the survival rate in the two groups of PSE and ASU was significantly lower than the control group (p ≤ 0.05). Quantitative western blotting showed that the production of collagen type II protein in both groups significantly increased compared to the control group (p ≤ 0.05). Quantitative analysis of collagen type X protein production showed that the production of this protein in the PSE group was significantly reduced compared to the ASU group.
Conclusion: PSE and ASU are two important factors in inducing chondrogenesis in hADSCs in fibrin scaffold. ASU's impact on chondrogenesis of hADSCs in fibrin scaffold is greater than that of PSE.
Materials and Methods: Human adipose-derived stem cells (hADSCs) were transferred to fibrin scaffolds in the third passage in three groups (control, PSE, and ASU) for chondrogenic differentiation. After 14 days, Western blotting evaluated the samples for cell survival by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay production of collagen type II and collagen type X.
Results: Evaluation of MTT results in different groups showed that the survival rate in the two groups of PSE and ASU was significantly lower than the control group (p ≤ 0.05). Quantitative western blotting showed that the production of collagen type II protein in both groups significantly increased compared to the control group (p ≤ 0.05). Quantitative analysis of collagen type X protein production showed that the production of this protein in the PSE group was significantly reduced compared to the ASU group.
Conclusion: PSE and ASU are two important factors in inducing chondrogenesis in hADSCs in fibrin scaffold. ASU's impact on chondrogenesis of hADSCs in fibrin scaffold is greater than that of PSE.
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