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International Journal of Medical Laboratory
Shahid Sadoughi University of Medical Sciences
Fri, Oct 31, 2025
[Archive]
Islamic Azad University, Science & Research Branch of Ahvaz, Iran
Abstract: (19 Views)
Introduction: The present study investigated the effect of six weeks of interval training and consumption of Prosopis farcta root extract (PFRE) on Glucagon-like peptide-1 (GLP-1) gene expression in the intestine of diabetic male rats.
Materials and Methods: In this study, 50 male Wistar rats were randomly divided into 5 groups (control, diabetes, diabetes + intermittent exercise, diabetes + PFRE, diabetes + exercise and PFRE). Diabetes was induced by intraperitoneal injection of streptozotocin (50 mg/kg). Interval training includes 5 to 12 repetitions at an intensity of 24 to 34 m/min (75-100% maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. PFRE was given to the animals at a dose of 300 mg/kg by the gavage method. After six weeks, the animals were anesthetized, and blood samples were taken directly from the hearts of the animals, and the duodenal part of their intestines was removed to evaluate the GLP-1 gene expression.
Results: Diabetes induction significantly increased plasma glucose concentration and GLP-1 gene expression (about 16 times the control group). Also, the concentration of plasma glucose decreased significantly after 6 weeks of intermittent training, consumption of PFRE, and the combination of exercise and PFRE. (P=0.0001) But no significant difference was observed between the intervention groups. Intermittent training (32.3%), PFRE (72.8%), and the combination of exercise and PFRE (92.6%) decreased GLP-1 gene expression compared to the diabetes group.
Conclusion: The results of the present study showed that interval training, PFRE, and the combination of two interventions reduce blood glucose. Also, GLP-1 gene expression was significantly reduced in the consumption of PFRE and the combination of exercise and PFRE, but interval training alone had no significant effect on GLP-1 gene expression.
Materials and Methods: In this study, 50 male Wistar rats were randomly divided into 5 groups (control, diabetes, diabetes + intermittent exercise, diabetes + PFRE, diabetes + exercise and PFRE). Diabetes was induced by intraperitoneal injection of streptozotocin (50 mg/kg). Interval training includes 5 to 12 repetitions at an intensity of 24 to 34 m/min (75-100% maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. PFRE was given to the animals at a dose of 300 mg/kg by the gavage method. After six weeks, the animals were anesthetized, and blood samples were taken directly from the hearts of the animals, and the duodenal part of their intestines was removed to evaluate the GLP-1 gene expression.
Results: Diabetes induction significantly increased plasma glucose concentration and GLP-1 gene expression (about 16 times the control group). Also, the concentration of plasma glucose decreased significantly after 6 weeks of intermittent training, consumption of PFRE, and the combination of exercise and PFRE. (P=0.0001) But no significant difference was observed between the intervention groups. Intermittent training (32.3%), PFRE (72.8%), and the combination of exercise and PFRE (92.6%) decreased GLP-1 gene expression compared to the diabetes group.
Conclusion: The results of the present study showed that interval training, PFRE, and the combination of two interventions reduce blood glucose. Also, GLP-1 gene expression was significantly reduced in the consumption of PFRE and the combination of exercise and PFRE, but interval training alone had no significant effect on GLP-1 gene expression.
Type of Study: Research |
Subject:
Genetics/ Biotechnology
Received: 2025/05/5 | Accepted: 2024/08/31
Received: 2025/05/5 | Accepted: 2024/08/31
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