International Journal of Medical Laboratory

Background and Aims: In this lab trial, the effect of scaffold based on human serum albumin (HSA) and hydroxyapatite nanoparticles (HA NPs) on mouse spermatogonial cell line (SCL) was investigated.
Materials and Methods: To synthesize HA NPs, calcium nitrate and diammonium phosphate at pH 13 were gently added and heated at 100 ºC for 24 hours. Then serial concentrations of HA NPs was separately added to 500 mg/mL of HSA, and immediately placed in the 100 ºC water bath. Then, all scaffolds were cut, and incubated with SCL for 6h, 12h, and 24h at 37 ºC. Finally, the cell count was read, and homing of the cells was examined by optical microscopy.
Results: It was found that the quantity of cells did not change by increase in concentration of HA NPs. On the other hand, increased incubation time led to decrease in cell count. Light microscopic observation of scaffold cavities after incubation showed the homing of spermatogonial cells.
Conclusions: This promising scaffold must be more investigated in vitro and in vivo, and may be suitable for making artificial testis.